Structural features unique to enteric adenoviruses

  • M. Brown
  • J. D. Grydsuk
  • E. Fortsas
  • M. Petric
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 12)


Enteric adenoviruses are important agents of pediatric gastroenteritis. Characterization of monoclonal antibodies against human adenovirus 41 (h-41) identified an epitope of interest on protein VI, an internal virion protein. The epitope is common to enteric adenoviruses (subgenus A: h-12, h-18, h-31 and subgenus F: h-40, h-41) but is not shared by non-enteric serotypes (subgenera B, C, D or E). By expressing random oligonucleotide fragments of the protein VI gene as T7 gene 10 fusion proteins in the pTope vector (Novagen), the epitope was mapped within the central domain of protein VI, to the region corresponding to aa 114-125 of the Ad2 protein. Identification of this epitope reflects the close evolutionary relationship of subgenus A and subgenus F adenoviruses and draws attention to structural features of enteric adenoviruses as potential determinants of tropism. Furthermore, this epitope may be valuable for identification of enteric adenoviruses in clinical specimens.


Short Fibre Stool Specimen Adenovirus Type Human Adenovirus Close Evolutionary Relationship 
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  1. 1.
    Ahluwalia GS, Scott-Taylor TH, Klisko B, Hammond GW (1994) Comparison of detection methods for adenovirus from enteric clinical specimens. Diag Microbiol Infec Dis 18: 161–166CrossRefGoogle Scholar
  2. 2.
    Bailey AC, MacKay N, Mautner V (1993) Enteric adenovirus type 40: Expression of E1B proteins in vitro and in vivo. Virol 193: 631–641CrossRefGoogle Scholar
  3. 3.
    Bailey A, Mautner V (1994) Phylogenetic relationships among adenovirus serotypes. Virol. 205: 438–452CrossRefGoogle Scholar
  4. 4.
    Bailey A, Ullah R, Mautner V (1994) Cell type specific regulation of expression from the Ad40 E1B promoter in recombinant Ad5/Ad40 viruses. Virol 202: 695–706CrossRefGoogle Scholar
  5. 5.
    Bern C, Glass RI (1994) Impact of diarrheal diseases worldwide. In: Kapikian AZ (ed) Viral infections of the gastrointestinal tract. Marcel Dekker, New York, pp 1–26Google Scholar
  6. 6.
    Brown M (1990) Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986. J Clin Microbiol 28: 1525–1529PubMedGoogle Scholar
  7. 7.
    Brown M, Petric M, Middleton PJ (1984) Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens. J Clin Microbiol 20: 334–338PubMedGoogle Scholar
  8. 8.
    Brown M, Wilson-Friesen HL, Doane F (1992) A block in release of progeny virus and a high particle-to-infectious unit ratio contribute to poor growth of enteric adenovirus types 40 and 41 in cell culture. J Virol 66: 3198–3205PubMedGoogle Scholar
  9. 9.
    Cruz JR, Caceres P, Cano F, Flores J, Barlett A, Torun B (1990) Adenovirus types 40 and 41 and rotaviruses associated with diarrhea in children from Guatemala. J Clin Microbiol 28: 1780–1784PubMedGoogle Scholar
  10. 10.
    Davison AJ, Telford EA, Watson MS, McBride K, Mautner V (1993) The DNA sequence of adenovirus type 40. J Mol Biol 234: 1308–1316PubMedCrossRefGoogle Scholar
  11. 11.
    de Jong J, Bijlsma K, Wermenbol AG, Verweij-Uijterwaal MW, van der Avoort HGAM, Wood DJ, Bailey AS, Osterhaus ADME (1993) Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes. J Clin Microbiol 31: 1562–1569PubMedGoogle Scholar
  12. 12.
    Goldman MJ, Wilson JM (1995) Expression of α vβ5 integrin is necessary for efficient adenovirus-mediated gene transfer in the human airway. J Virol 69: 5951–5958PubMedGoogle Scholar
  13. 13.
    Grimwood K, Carzino R, Barnes GL, Bishop RF (1995) Patients with enteric adenovirus gastroenteritis admitted to an Australian pediatric teaching hospital from 1981 to 1992. J Clin Microbiol 33: 131–136PubMedGoogle Scholar
  14. 14.
    Grydsuk JD, Fortsas E, Petric M, Brown M (1996) Common epitope on protein VI of enteric adenoviruses from subgenus A and F. J Gen Virol 77: 1811–1819PubMedCrossRefGoogle Scholar
  15. 15.
    Herrmann JE, Perron-Henry DM, Blacklow NR (1987) Antigen detection with monoclonal antibodies for the diagnosis of adenovirus gastroenteritis. J Infect Dis 155: 1167–1171PubMedCrossRefGoogle Scholar
  16. 16.
    Hierholzer JC (1992) Adenoviruses in the immunocompromised host. Clin Microbiol Rev 5: 262–274PubMedGoogle Scholar
  17. 17.
    Hierholzer JC, Stone YO, Broderson JR (1991) Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch Virol 121: 179–197PubMedCrossRefGoogle Scholar
  18. 18.
    Jarecki-Khan K, Tsipori SR, Unicomb LE (1993) Enteric adenovirus infection among infants with diarrhea in rural Bangladesh. J Clin Microbiol 31: 484–489PubMedGoogle Scholar
  19. 19.
    Johansson ME, Andersson MA, Thorner, PA (1994) Adenoviruses isolated in the Stockholm area during 1987-1992: restriction endonuclease analysis and molecular epidemiology. Arch Virol 137: 101–115PubMedCrossRefGoogle Scholar
  20. 20.
    Johansson ME, Brown M, Hierholzer JC, Thorner A, Ushijma H, Wadell G (1991) Genome analysis of adenovirus type 31 strains from immunocompromised and immunocompetent patients. J Infect Dis 163: 293–299PubMedCrossRefGoogle Scholar
  21. 21.
    Kidd AH, Chroboczek J, Cusack S, Ruigrok RWH (1993) Adenovirus type 40 virions contain two distinct fibers. Virol 192: 73–84CrossRefGoogle Scholar
  22. 22.
    Kidd AH, Garwicz D, Oberg M (1995) Human and simian adenoviruses: Phylogenetic inferences from analysis of VA RNA genes. Virol 207: 32–45CrossRefGoogle Scholar
  23. 23.
    Krajden M, Brown M, Petrasek A, Middleton PJ (1990) Clinical features of adenovirus enteritis: a review of 127 cases. Pediatr Infect Dis J 9: 636–641PubMedGoogle Scholar
  24. 24.
    Lew JF, Moe CL, Monroe SS, Allen JR, Harrison BM, Forrester BD, Stine SE, Woods PA, Hierholzer JC, Herrmann JE, Blacklow NR, Bartlett AV, Glass RI (1991) Astro virus and adenovirus associated with diarrhea in children in day care settings. J Infect Dis 164: 673–678PubMedCrossRefGoogle Scholar
  25. 25.
    Mathias P, Wickham T, Moore M, Nemerow G (1994) Multiple adenovirus serotypes use αv integrins for infection. J Virol 68: 6811–6814PubMedGoogle Scholar
  26. 26.
    Matthews DA, Russell WC (1995) Adenovirus protein-protein interactions: molecular parameters governing the binding of protein VI to hexon and the activation of the adenovirus 23K protease. J Gen Virol 76: 1959–1969PubMedCrossRefGoogle Scholar
  27. 27.
    Mautner V, MacKay N, Steinthorsdottir V (1989) Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12. Virol 171: 619–622CrossRefGoogle Scholar
  28. 28.
    Mautner V, Steinthorsdottir V, Bailey A (1995) Enteric adenoviruses. In: Doerfler W, Bohm P (ed) The Molecular Repertoire of Adenoviruses. Springer, HeidelbergGoogle Scholar
  29. 29.
    Mickan LD, Kok TW (1994) Recognition of adenovirus types in faecal samples by Southern hybridization in South Australia. Epidemiol Infect 112: 603–613PubMedCrossRefGoogle Scholar
  30. 30.
    Noel J, Mansoor A, Thaker U, Herrmann J, Perron-Henry D, Cubitt WD (1994) Identification of adenoviruses in faeces from patients with diarrhoea at the Hospitals for Sick Children, London, 1989-1992. J Med Virol 43: 84–90PubMedCrossRefGoogle Scholar
  31. 31.
    Rodriguez FH, Liuzza GE, Gohd RH (1984) Disseminated adenovirus 31 infection in an immunocompromised host. Am J Clin Pathol 82: 615–618PubMedGoogle Scholar
  32. 32.
    Schnurr D, Dondero ME (1993) Two new candidate adenovirus serotypes. Intervirology 36: 79–83PubMedGoogle Scholar
  33. 33.
    Schnurr D, Bollen A, Crawford-Miksza L, Dondero ME, Yagi S (1995) Adenovirus mixture isolated from the brain of an AIDS patient with encephalitis. J Med Virol 47: 168–171PubMedCrossRefGoogle Scholar
  34. 34.
    Schofield KP, Morris DJ, Bailey AS, de Jong JC, Corbitt G (1994) Gastroenteritis due to adenovirus type 41 in an adult with chronic lymphocytic leukemia. Clin Infect Dis 19: 311–312PubMedCrossRefGoogle Scholar
  35. 35.
    Scott-Taylor TH, Hammond GW (1995) Local succession of adenovirus strains in pediatric gastroenteritis. J Med Virol 45: 331–338PubMedCrossRefGoogle Scholar
  36. 36.
    Stewart PL, Fuller SD, Burnett RM (1993) Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. EMBO J 12: 2589–2593PubMedGoogle Scholar
  37. 37.
    Tiemessen CT, Kidd AH (1994) Adenovirus type 40 and 41 growth in vitro: host range diversity reflected by differences in patterns of DNA replication. J Virol 68: 1239–1244PubMedGoogle Scholar
  38. 38.
    Tiemessen CT, Kidd AH (1995) The subgroup F adenoviruses. J Gen Virol 76: 481–497PubMedCrossRefGoogle Scholar
  39. 39.
    Van R, Wun C-C, O’Ryan M, Matson DO, Jackson L, Pickering LK (1992) Outbreaks of human enteric adenovirus types 40 and 41 in Houston day care centers. J Pediatr 120: 516–521PubMedCrossRefGoogle Scholar
  40. 40.
    van der Avoort HGAM, Wermenbol AG, Zomerdijk TPL, Kleijne JAFW, van Asten JAAM, Jensma P, Osterhaus ADME, Kidd AH, de Jong JC (1989) Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies. Vir Res 12: 139–158CrossRefGoogle Scholar
  41. 41.
    Wadell G, Allard A, Johansson M, Svensson L, Uhnoo I (1994) Enteric adenoviruses. In: Kapikian AZ (ed) Viral infections of the gastrointestinal tract. Marcel Dekker, New York, pp 519–547Google Scholar
  42. 42.
    Webster A, Hay RT, Kemp G (1993) The adenovirus protease is activated by a virus-coded disulphide-linked peptide. Cell 72: 97–104PubMedCrossRefGoogle Scholar
  43. 43.
    White JM (1993) Integrins as virus receptors. Current Biology 3: 596–599PubMedCrossRefGoogle Scholar
  44. 44.
    Wickham TJ, Mathias P, Cheresh DA, Nemerow GR (1993) Integrins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachment. Cell 73: 309–319PubMedCrossRefGoogle Scholar
  45. 45.
    Wood DJ, Bijlsma K, de Jong JC, Tonkin C (1989) Evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens. J Clin Microbiol 27: 1155–1158PubMedGoogle Scholar
  46. 46.
    Yeh HY, Pieniazek N, Pieniazek D, Gelderblom H, Luftig RB (1994) Human adenovirus type 41 contains two fibers. Virus Res 33: 179–198PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 1996

Authors and Affiliations

  • M. Brown
    • 1
    • 3
  • J. D. Grydsuk
    • 1
  • E. Fortsas
    • 1
  • M. Petric
    • 1
    • 2
  1. 1.Department of MicrobiologyUniversity of TorontoTorontoCanada
  2. 2.Department of MicrobiologyThe Hospital for Sick ChildrenTorontoCanada
  3. 3.Department of Medical GeneticsUniversity of TorontoTorontoCanada

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