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Structure of Norwalk virus

  • Conference paper
Viral Gastroenteritis

Part of the book series: Archives of Virology ((ARCHIVES SUPPL,volume 12))

Summary

Norwalk virus is the major cause of epidemic viral gastroenteritis of humans. Attempts to grow this virus in laboratory cell lines have been unsuccessful. However, the Norwalk virus capsid protein, when expressed in insect cells infected with a recombinant baculovirus, spontaneously assembles into viruslike particles. We have determined the 3-dimensional structure of baculovirus-expressed Norwalk virus using electron cryomicroscopy and computer image reconstruction to a resolution of ~ 22 Å. These particles, having a diameter of 380 Å exhibit T = 3 icosahedral symmetry. The 3-dimensional structure is composed of 90 dimers of the 58 000 molecular weight (58 K) capsid protein, each of which forms an arch-like capsomere. The structure of the protein subunit is modular with three distinct domains. The distal globular domain that appears bilobed is connected to the lower shell domain by a central stem-like domain. We also have been able to grow crystals of the baculovirus-expressed Norwalk virus particles suitable for high resolution X-ray crystallography.

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Author information

Authors and Affiliations

  1. Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX, 77030, USA

    Dr. B. V. V. Prasad

  2. Division of Molecular Virology, Baylor College of Medicine, Houston, Texas, USA

    M. E. Hardy, X. Jiang & M. K. Estes

  3. Center for Pediatric Research, Eastern Virginia Medical School, Norfolk, Virginia, USA

    X. Jiang

Authors
  1. Dr. B. V. V. Prasad
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  2. M. E. Hardy
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  3. X. Jiang
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  4. M. K. Estes
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Editor information

Editors and Affiliations

  1. Sapporo Medical University, Sapporo, Japan

    Shunzo Chiba  & Shuji Nakata  & 

  2. Baylor College of Medicine, Houston, USA

    Mary K. Estes

  3. Colorado State University, Fort Collins, USA

    Charles H. Calisher

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© 1996 Springer-Verlag Wien

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Prasad, B.V.V., Hardy, M.E., Jiang, X., Estes, M.K. (1996). Structure of Norwalk virus. In: Chiba, S., Estes, M.K., Nakata, S., Calisher, C.H. (eds) Viral Gastroenteritis. Archives of Virology, vol 12. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6553-9_25

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  • DOI: https://doi.org/10.1007/978-3-7091-6553-9_25

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82875-5

  • Online ISBN: 978-3-7091-6553-9

  • eBook Packages: Springer Book Archive

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