Structure of Norwalk virus

  • B. V. V. Prasad
  • M. E. Hardy
  • X. Jiang
  • M. K. Estes
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 12)


Norwalk virus is the major cause of epidemic viral gastroenteritis of humans. Attempts to grow this virus in laboratory cell lines have been unsuccessful. However, the Norwalk virus capsid protein, when expressed in insect cells infected with a recombinant baculovirus, spontaneously assembles into viruslike particles. We have determined the 3-dimensional structure of baculovirus-expressed Norwalk virus using electron cryomicroscopy and computer image reconstruction to a resolution of ~ 22 Å. These particles, having a diameter of 380 Å exhibit T = 3 icosahedral symmetry. The 3-dimensional structure is composed of 90 dimers of the 58 000 molecular weight (58 K) capsid protein, each of which forms an arch-like capsomere. The structure of the protein subunit is modular with three distinct domains. The distal globular domain that appears bilobed is connected to the lower shell domain by a central stem-like domain. We also have been able to grow crystals of the baculovirus-expressed Norwalk virus particles suitable for high resolution X-ray crystallography.


Capsid Protein Recombinant Baculovirus Icosahedral Symmetry Tomato Bushy Stunt Virus Norwalk Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Wien 1996

Authors and Affiliations

  • B. V. V. Prasad
    • 1
  • M. E. Hardy
    • 2
  • X. Jiang
    • 2
    • 3
  • M. K. Estes
    • 2
  1. 1.Verna and Marrs McLean Department of BiochemistryBaylor College of MedicineHoustonUSA
  2. 2.Division of Molecular VirologyBaylor College of MedicineHoustonUSA
  3. 3.Center for Pediatric ResearchEastern Virginia Medical SchoolNorfolkUSA

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