Advertisement

DNA vaccines against rotavirus infections

  • J. E. Herrmann
  • S. C. Chen
  • E. F. Fynan
  • J. C. Santoro
  • H. B. Greenberg
  • H. L. Robinson
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 12)

Summary

Plasmid DNA vaccines encoding for murine rotaviral proteins VP4, VP6, and VP7 were tested in adult BALB/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. Serum antibodies were measured by virus neutralization and by ELISA. Cellular immunity was assessed by measuring cytotoxic T cell (CTL) responses. The vaccines were administered by inoculation into cells of the epidermis with an Accell gene gun (Auragen, Inc., Middleton, WI, USA). Each of the three vaccines elicited rotavirus-specific serum antibodies as measured by ELISA. Virus neutralizing antibodies were detected in mice receiving DNA vaccines encoding for VP4 and VP7, but not in those which received the plasmid encoding for VP6. Vaccines encoding for VP4, VP6, or VP7 generated virus-specific CTL responses in recipient mice. Efficacy of the vaccines was determined by challenge with homotypic rotaviruses. Each of the three vaccines was effective in protecting mice against infection after rotavirus (100 ID50) challenge. Significant reductions (p < 0.0002, analysis of variance) in viral excretion measured over a 9 day period were seen in mice receiving the DNA vaccines compared with mice that received control plasmids.

Keywords

Rabies Virus Gold Bead Adult Mouse Model 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bishop RF (1993) Development of candidate rotavirus vaccines. Vaccine 11: 247–254PubMedCrossRefGoogle Scholar
  2. 2.
    Both GW, Lockett LJ, Janardhana V, Edwards SJ, Bellamy AR, Graham FL, Prevec L, Andrew ME (1993) Protective immunity to rotavirus-induced diarrhea is passively transferred to newborn mice from naive dams vaccinated with a single dose of a recombinant adenovirus expressing rotavirus VP7sc. Virology 193: 940–950PubMedCrossRefGoogle Scholar
  3. 3.
    Conner ME, Crawford SE, Barone C, Estes MK (1993) Rotavirus vaccine administered parenterally induces protective immunity. J Virol 67: 6633–6641PubMedGoogle Scholar
  4. 4.
    Conner ME, Matson DO, Estes MK (1994) Rotavirus vaccines and vaccination potential. In: Ramig RF (ed) Rotaviruses. Current Topics in Microbiology and Immunology 185: 285–371Google Scholar
  5. 5.
    Dharakul T, Labbe M, Cohen J, Bellamy AR, Street JE, Mackow ER, Fiore L, Rott L, Greenberg HB (1991) Immunization with baculovirus-expressed recombinant rotavirus proteins VP1, VP4, VP6 and VP7 induce CD8 + T lymphocytes that mediate clearance of chronic rotavirus infection in SCID mice. J Virol 65: 5928–5932PubMedGoogle Scholar
  6. 6.
    Dormitzer P, Burns JW, Greenberg HB, Prevec L, Graham F, Boyle DB, Both G (1993) Vaccination of adult mice with recombinant viruses expressing a protective rotavirus VP7sc. Abstr. IXth Intl. Cong. Virology, W21-2, p43Google Scholar
  7. 7.
    Estes MR, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449PubMedGoogle Scholar
  8. 8.
    Feng N, Burns JW, Bracy L, Greenberg HB (1994) Comparison of mucosal and systemic humoral immune responses and subsequent protection in mice orally inoculated with a homologous or a heterologous rotavirus. J Virol 68: 7766–7733PubMedGoogle Scholar
  9. 9.
    Fynan EF, Webster RG, Fuller DH, Haynes JR, Santoro JC, Robinson HL (1993) DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc Natl Acad Sci USA 90: 11478–11482PubMedCrossRefGoogle Scholar
  10. 10.
    Burns JW, Pajouh MS, Krishnaney AA, Greenberg HB (1996) Protective effect of rotavirus VP6-specific IgA monoclonal antibodies that lack neutralizing activity. Science 272: 104–107PubMedCrossRefGoogle Scholar
  11. 11.
    Herrmann JE, Blacklow NR, Perron DM, Cukor G, Krause PJ, Hyams JS, Barrett HJ, Ogra PL (1985) Enzyme immunoassay with monoclonal antibodies for the detection of rotavirus in stool specimens. J Infect Dis 152: 831–832CrossRefGoogle Scholar
  12. 12.
    Herrmann JE, West K, Bruns M, Ennis FA (1990) Effect of rimantadine on cytotoxic T lymphocyte responses and immunity to reinfection in mice infected with influenza A virus. J Infect Dis 161: 180–184PubMedCrossRefGoogle Scholar
  13. 13.
    Kapikian AZ, Flores J, Midthun K, Hoshino Y, Green KY, Gorziglia M, Nishikawa K, Chanock RM, Potash L, Perez-Schael I (1989) Strategies for the development of a rotavirus vaccine against infantile diarrhea with an update on clinical trials of rotavirus vaccine. Adv Exp Med Biol 257: 67–89PubMedCrossRefGoogle Scholar
  14. 14.
    Mazenec MB, Kaetzel CS, Lamm ME, Fletcher D, Nedrud JG (1992) Intracellular neutralization of virus by immunoglobulin A antibodies. Proc Natl Acad Sci USA 89: 6901–6905CrossRefGoogle Scholar
  15. 15.
    McNeal MM, Sheridan JR, Ward RL (1992) Active protection against rotavirus infection of mice following intraperitoneal immunization. Virology 191: 150–157PubMedCrossRefGoogle Scholar
  16. 16.
    Offit PA, Dudzik KI (1988) Rotavirus-specific cytotoxic T lymphocytes cross-react with target cells infected with different rotavirus serotypes. J Virol 62: 127–131PubMedGoogle Scholar
  17. 17.
    Riepenhoff-Talty M, Dharakul R, Kowalski E, Sterman D, Ogra PL (1987) Rotavirus infection in mice: pathogenesis and immunity. Adv Exp Med Biol 216B: 1015–1023Google Scholar
  18. 18.
    Saif LJ, Jackwood DJ (1990) Enteric Virus Vaccines: Theoretical considerations, current status, and future approaches. In: Saif LJ, Theil KW (eds) Viral Diarrheal of Man and Animals. CRC Press, Boca Raton, pp 313–329Google Scholar
  19. 19.
    Ulmer JB, Donnelly JJ, Parker SE, Rhodes GH, Feigner PI, Dwarki VJ, Gromkowski SH, Deck RR, DeWitt CM, Friedman A, Hawe LA, Leander KR, Martinez D, Perry HC, Shiver JW, Montgomery DL, Liu MA (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259: 1745–1749PubMedCrossRefGoogle Scholar
  20. 20.
    Ward RL, McNeal MM, Sheridan JF (1990) Development of an adult mouse model for studies on protection against rotavirus. J Virol 64: 5070–5075PubMedGoogle Scholar
  21. 21.
    Webster RG, Fynan EF, Santoro JC, Robinson H (1994) Protection of ferrets against influenza challenge with a DNA vaccine to the haemagglutinin. Vaccine 12: 1495–1498PubMedCrossRefGoogle Scholar
  22. 22.
    Xiang ZO, Spitalnik S, Tran M, Wunner W, Cheng J, Ertl HCL (1994) Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology 199: 132–140PubMedCrossRefGoogle Scholar
  23. 23.
    Zarozinski CC, Fynan EF, Selin LK, Robinson HL, Welsh RM (1995) Protective CTL-dependent immunity and enhanced immunopathology in mice immunized by particle bombardment with DNA encoding an internal protein. J Immunol 154: 4010–4017PubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 1996

Authors and Affiliations

  • J. E. Herrmann
    • 1
  • S. C. Chen
    • 1
  • E. F. Fynan
    • 1
    • 3
  • J. C. Santoro
    • 2
  • H. B. Greenberg
    • 4
  • H. L. Robinson
    • 2
  1. 1.Division of Infectious Diseases and ImmunologyUniversity of Massachusetts Medical CenterWorcesterUSA
  2. 2.Department of PathologyUniversity of Massachusetts Medical SchoolWorcesterUSA
  3. 3.Department of BiologyWorcester State CollegeWorcesterUSA
  4. 4.Division of GastroenterologyStanford University School of MedicineStanfordUSA

Personalised recommendations