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Development of rotavirus molecular epidemiology: electropherotyping

  • Conference paper
Viral Gastroenteritis

Part of the book series: Archives of Virology ((ARCHIVES SUPPL,volume 12))

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Summary

Early in the era of rotavirology it was realized that the characteristic patterns of bands produced in polyacrylamide gels following electrophoresis of genomic dsRNA were useful for checking the identity of rotavirus isolates. However it was Romilio Espejo who first proposed the use of this technique for epidemiology, although most others did not take the suggestion seriously because the technique was then rather specialized and RNA staining methods were not very sensitive. Using samples collected by Ruth Bishop in Melbourne following the original identification of human rotaviruses, Sue Rodger recorded the “electropherotypes” of all samples available to 1979 and painstakingly compared them, side by side (since minor variations in conditions, especially temperature, alter the relative migration distances of dsRNA bands). These efforts produced the first longitudinal, extensive study of human rotavirus strain variation. Since then, technical improvements have greatly increased the sensitivity of the procedures, and electropherotyping has been recognized as a powerful and economical method for epidemiological studies of rotaviruses.

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Authors and Affiliations

  1. Department of Microbiology, University of Melbourne, Parkville, Victoria, 3052, Australia

    Dr. I. H. Holmes

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  1. Dr. I. H. Holmes
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Editor information

Editors and Affiliations

  1. Sapporo Medical University, Sapporo, Japan

    Shunzo Chiba  & Shuji Nakata  & 

  2. Baylor College of Medicine, Houston, USA

    Mary K. Estes

  3. Colorado State University, Fort Collins, USA

    Charles H. Calisher

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© 1996 Springer-Verlag Wien

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Holmes, I.H. (1996). Development of rotavirus molecular epidemiology: electropherotyping. In: Chiba, S., Estes, M.K., Nakata, S., Calisher, C.H. (eds) Viral Gastroenteritis. Archives of Virology, vol 12. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6553-9_10

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  • DOI: https://doi.org/10.1007/978-3-7091-6553-9_10

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82875-5

  • Online ISBN: 978-3-7091-6553-9

  • eBook Packages: Springer Book Archive

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