Viral zoonosis from the viewpoint of their epidemiological surveillance: tick-borne encephalitis as a model

  • J. Süss
  • P. Béziat
  • Ch. Schrader
Conference paper


Tick-borne encephalitis (TBE) is a vector borne and, more rarely, a food (milk, milk products) borne disease of humans. For further characterization of the virus activity in natural foci of TBE more than 32 000 unengorged wild ticks were caught in low and high virus active foci in Germany (Mecklenburg-Western Pomerania, Saxony, Brandenburg, Thuringia, Bavaria, Baden-Württemberg, Saarland). The ticks were examined by RT-PCR and Southern blot hybridization as well as by classical virological methods. The dynamics of such natural foci of TBE in the last 35 years were discussed. Also nucleotide sequence data of parts of the virus genome (5′- non coding region) of 16 European and some Far East subtype strains were compared.


TBEV Strain European Subtype Powassan Virus Tick Pool Chick Embryo Cell Culture 
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  1. 1.
    Apitzsch L, Sinnecker H, Wigand R, Berndt D (1968) Zeckencephalitis-Virusisolierungen in der DDR 1965/66 und einige Stammdifferenzierungen. Zbl Bakt I Orig 207: 429–434Google Scholar
  2. 2.
    Blok J, McWilliam SM, Butler HC, Gibbs AJ, Weiller G, Herring BL, Hemsley AC, Aaskov Jg, Yoksan S, Bhamarapravati N (1992) Comparison of a Dengue-2 virus and its candidate vaccine derivative: sequence relationships with the flaviviruses and other viruses. Virology 187: 573–590PubMedCrossRefGoogle Scholar
  3. 3.
    Brinton MA, Fernandez AV, Dispoto JH (1986) The 3′-nucleotides of flavivirus genomic RNA form a conserved secondary structure. Virology 153: 113–121PubMedCrossRefGoogle Scholar
  4. 4.
    Brinton MA, Dispoto JH (1988) Sequence and secondary structure analysis of the 5′-terminal region of flavivirus genome RNA. Virology 162: 290–299PubMedCrossRefGoogle Scholar
  5. 5.
    Calisher CH, Karabatsos N, Dalrymple JM, Shope RE, Porterfield JS, Westaway EG, Brandt WE (1989) Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. J Gen Virol 70: 37–43PubMedCrossRefGoogle Scholar
  6. 6.
    Chambers TJ, Hahn CS; Galler R, Rice CM (1990) Flavivirus genome, organization, expression and replication. Ann Rev Microbiol 44: 649–688CrossRefGoogle Scholar
  7. 7.
    Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159PubMedCrossRefGoogle Scholar
  8. 8.
    DeMadrid AT, Porterfield JS (1974) The flaviviruses (Group B Arboviruses): a cross-neutralization study. J Gen Virol 23: 91–96CrossRefGoogle Scholar
  9. 9.
    Deubel V, Kinney RM, Trent DW (1986) Nucleotide sequence and deduced amino acid sequence of the structural proteins of Dengue type 2 virus, Jamaica genotype. Virology 155: 365–377PubMedCrossRefGoogle Scholar
  10. 10.
    Francki RIB, Fauquet CM, Knudson DL, Brown F (eds) (1991) Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 223–233 (Arch Virol [Suppl] 2)Google Scholar
  11. 11.
    Gao GF, Hussain MH, Reid HW, Gould EA (1993a) Classification of a new member of the TBE flavivirus subgroup by its immunological, pathogenic and molecular characteristics: Identification of subgroup-specific pentapeptides. Virus Res 30: 129–144PubMedCrossRefGoogle Scholar
  12. 12.
    Gao GF, Jiang WR, Hussain MH, Venugopal K, Gritsun TS, Reid HW, Gould EA (1993b) Sequencing and antigenic studies of a Norwegian virus isolated from encephalomyelitic sheep confirm the existence of Louping ill virus outside Great Britain and Ireland. J Gen Virol: 109–114Google Scholar
  13. 13.
    Gritsun TS, Frolova TV, Pogodina VV, Lashkevich VA, Venogopal K, Gould EA (1993a) Nucleotide and deduced amino acid sequence of the envelope gene of the Vasilchenko strain of TBE virus, comparison with other flavivirus. Virus Res 27: 201–209PubMedCrossRefGoogle Scholar
  14. 14.
    Gritsun TS, Lashkevich VA, Gould EA (1993b) Nucleotide and deduced amino acid sequence of the envelope glycoprotein of Omsk haemorrhagic fever, comparison with other flaviviruses. J Gen Virol 74: 287–291PubMedCrossRefGoogle Scholar
  15. 15.
    Hahn CS, Hahn YS, Rice CM, Lee E, Dalgarno L, Strauss EG, Strauss JH (1987) Conserved elements in the 3′untranslated region of flavivirus RNAs and potential cyclization sequences. J Mol Biol 198; 33–41PubMedCrossRefGoogle Scholar
  16. 16.
    Heinz FX, Kunz C (1981) Homogeneity of the structural glycoproteins from European isolates of tick-borne encephalitis virus: comparison with other flaviviruses. J Gen Virol 57: 263–274PubMedCrossRefGoogle Scholar
  17. 17.
    Heinz FX, Berger R, Tuma W, Kunz C (1983) A topological and functional model of epitopes on the structural glycoprotein of Tick-borne encephalitis virus defined by monoclonal antibodies. Virology 126: 525–537PubMedCrossRefGoogle Scholar
  18. 18.
    Heinz FX, Mandl CW, Guirakhoo F, Holzmann H, Tuma W, Kunz C (1990) The envelope protein E of tick-borne encephalitis virus and other flaviviruses: structure, functions and evolutionary relationships. Arch Virol [Suppl] 1: 125–135Google Scholar
  19. 19.
    Jacobs SC, Stephenson JR, Wilkinson GWG (1993) Sequence of the NS 1 gene of the K23 isolate of Tick-borne encephalitis virus and identification of conserved motifs. Arch Virol 129: 279–285PubMedCrossRefGoogle Scholar
  20. 20.
    Kaiser R, Kern A, Fressle R, Steinbrecher A, Omram H, Malzacher V, Kügler D, Kampa D, Batsford S (1996) Zeckenvermittelte Erkrankungen in Baden-Württemberg. Münch Med Wochenschr 138: 647–652Google Scholar
  21. 21.
    Mandl CW, Heinz, FX, Kunz C (1988) Sequence of the structural proteins of tick-borne encephalitis virus (western subtype) and comparative analysis with other flaviviruses. Virology 166: 197–205PubMedCrossRefGoogle Scholar
  22. 22.
    Mandl CW, Heinz FX, Stöckl E, Kunz C (1989) Genome sequence of tick-borne encephalitis virus (western subtype) and comparative analysis of non-structural proteins with other flaviviruses. Virology 173; 291–301PubMedCrossRefGoogle Scholar
  23. 23.
    Mandl CW, Kunz, C, Heinz, FX (1991a) Presence of poly(A) in a flavivirus: significant differences between the 3′ non coding regions of the genomic RNAs of tick-borne encephalitis virus strains. J Virol 65: 4070–4077PubMedGoogle Scholar
  24. 24.
    Mandl CW, Iacono-Connors L, Wallner G, Holzmann H, Kunz C, Heinz FX (1991b) Sequence of the genes encoding the structural proteins of the low-virulence tick-borne flaviviruses Langat TP 21 and Yelantsev. Virology 185: 891–895PubMedCrossRefGoogle Scholar
  25. 25.
    Mandl CW, Holzmann H, Kunz C, Heinz FX (1993) Complete genomic sequence of Powassan virus: evaluation of genetic elements in tick-borne versus mosquito-borne flaviviruses. Virology 194: 173–184PubMedCrossRefGoogle Scholar
  26. 26.
    Monath TP, Heinz FX (1996) Flaviviruses. In: Fields BN, Knipe DM, Howley PM (eds) Virology. Lippincott Raven, Philadelphia New York, pp 961–1034Google Scholar
  27. 27.
    Pletnev AG, Yamshchikov VF, Blinov VM (1986) Tick-borne encephalitis virus genome. The nucleotide sequence coding for virion structural proteins. FEBS Lett: 317–321Google Scholar
  28. 28.
    Pletnev AG, Yamshchikov VF, Blinov VM (1990) Nucleotide sequence of the genome and complete amino acid sequence of the polyprotein of Tick-borne encephalitis virus. Virology 174: 250–263PubMedCrossRefGoogle Scholar
  29. 29.
    Porterfield JS (1994) Encephalitis viruses. In: Webster RG, Granoff A (eds) Encyclopedia of virology, vol 1. Academic Press, London, pp 361–372Google Scholar
  30. 30.
    Ramelow C, Süss J, Berndt D, Roggendorf M, Schreier E (1993) Detection of tick-borne encephalitis virus RNA in ticks (Ixodes ricinus) by the polymerase chain reaction. J Virol Methods 45: 115–119PubMedCrossRefGoogle Scholar
  31. 31.
    Roggendorf M, Lenz P (1994) Änderungen der Epidemiologie der FSME in Süddeutschland. Ellipse 11: 33–40Google Scholar
  32. 32.
    Roggendorf M, Lenz P, Kaiser R, Jäger G (1995) Epidemiologische Situation der FSME in Bayern und Baden-Württemberg. In: Süss J (ed) Durch Zecken übertragbare Erkrankungen FSME und Lyme-Borreliose. Weller, Schriesheim, pp 7–19Google Scholar
  33. 33.
    Rice CM, Strauss EG, Strauss JH (1986) Structure of the flavivirus genome. In: Schlesinger S, Schlesinger MJ (eds) The Togaviridae and Flaviviridae. Plenum Press, New York London, pp 279–326CrossRefGoogle Scholar
  34. 34.
    Safronov PF, Netesov SV, Mikryukova TP, Blinov VM, Osipova EG, Kiseleva NN, Sandakhchiev LS (1991) Nucleotide sequence of genes and complete amino acid sequence of proteins of tick-borne encephalitis virus strain 205. Mol Gen Microbiol Virusol 4: 23–29Google Scholar
  35. 35.
    Shamanin VA, Pletnev AG, Rubin SG, Zlobin VI (1990) Differentiation of strains of tick-borne encephalitis virus by means of RNA-DNA hybridization. J Gen Virol 71: 1505–1515PubMedCrossRefGoogle Scholar
  36. 36.
    Shiu SYW, Ayres MD, Gould EA (1991) Genomic sequence of the structural proteins of Louping ill virus: comparative analysis with tick-borne encephalitis virus. Virology 180: 411–415PubMedCrossRefGoogle Scholar
  37. 37.
    Sinnecker H (1986) Tick-borne encephalitis — German Democratic Republic. In: Tick-borne encephalitis and haemorrhagic fever with renal syndrome in Europe — Report on a WHO meeting 1983. EURO Rep Studies 104: 19–23Google Scholar
  38. 38.
    Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517PubMedCrossRefGoogle Scholar
  39. 39.
    Stephenson JR, Lee JM, Wilton-Smith PD (1984) Antigenic variation among members of the tick-borne encephalitis complex. J Gen Virol 65: 81–89PubMedCrossRefGoogle Scholar
  40. 40.
    Strauss EG, Strauss JH (1983) Replication strategies of the single-stranded RNA viruses of eukaryotes. Curr Top Microbiol Immunol 105: 1–98PubMedCrossRefGoogle Scholar
  41. 41.
    Süss J, Sinnecker H, Sinnecker R, Berndt D, Zilske E, Dedek G, Apitzsch L (1992) Epidemiology and ecology of tick-borne encephalitis in the eastern part of Germany between 1960 and 1990 and studies on the dynamics of a natural focus of tick-borne encephalitis. Zbl Bakt 277: 224–235Google Scholar
  42. 42.
    Süss J, Béziat P, Ramelow C, Berndt D (1995) Untersuchungen zur epidemiologischen Situation der FSME in den neuen Bundesländern von 1992–1994. In: Süss J (ed) Durch Zecken übertragbare Erkrankungen FSME und Lyme-Borreliose. Weller, Schriesheim, pp 25–32Google Scholar
  43. 43.
    Süss J, Béziat P, Ramelow C, Kahl O (1997) Tick-borne encephalitis virus (TBEV)-specific RT-PCR for characterization of natural foci of TBE and some other applications. Zbl Bakt 286: 125–138Google Scholar
  44. 44.
    Treib J (1994) First case of tick-borne encephalitis (TBE) in the Saarland. Infection 22: 368–369PubMedCrossRefGoogle Scholar
  45. 45.
    Varelas-Wesley I, Calisher CH (1982) Antigenic relationships of flaviviruses with undetermined arthropod-borne status. Am J Trop Med Hyg 31: 1273–1284PubMedGoogle Scholar
  46. 46.
    Venugopal K, Buckley A, Reid HW, Gould EA (1992) Nucleotide sequence of the envelope glycoprotein of Negishi virus shows very close homology to Louping ill virus. Virology 190: 515–521PubMedCrossRefGoogle Scholar
  47. 47.
    Westaway EG, Brinton MA, Gaidamovich SY, Horzinek MC, Igarashi A, Kääriäinen L, Lvov DK, Porterfield JS, Russell PK, Trent DW (1985) Flaviviridae. Intervirology 24: 183–192PubMedCrossRefGoogle Scholar
  48. 48.
    Whitby JE, Jennings AD, Barrett ADT (1993a) Nucleotide sequence of the envelope protein gene of the tick-borne flavivirus, Kumlinge A52. Virus Genes 7: 145–149PubMedCrossRefGoogle Scholar
  49. 49.
    Whitby JE, Whitby SN, Jennings AD, Stephenson JR, Barrett ADT (1993b) Nucleotide sequence of the envelope protein of a Turkish isolate of tick-borne encephalitis (TBE) virus is distinct from other viruses of the TBE virus complex. J Gen Virol 74: 921–924PubMedCrossRefGoogle Scholar
  50. 50.
    Yamshchikov VF, Pletnev AG (1988) Nucleotide sequence of the genome region encoding the structural proteins and the NS1 protein of the tick-borne encephalitis virus. Nucleic Acids Res 16: 7750PubMedCrossRefGoogle Scholar
  51. 51.
    Zhao B, Mackow E, Buckler-White A, Markoff L, Chanock RM, Lai CJ, Makino Y (1986) Cloning full-length Dengue type 4 viral DNA sequences: analysis of genes coding for structural proteins. Virology 155: 77–88PubMedCrossRefGoogle Scholar
  52. 52.
    Zlobin VI, Shamanin VA, Pletnev AG, Drokin DA, Dzhioev YP, Voronko IV, Melnikova OV, Gusarova NA (1992) Specific reactivity of kDNA-and desoxyoligonucleotide probes complementary to the genome of tick-borne encephalitis virus with RNA of the strains of different geographical origin. Vopr Virusol 37: 248–252PubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 1997

Authors and Affiliations

  • J. Süss
    • 1
    • 2
  • P. Béziat
    • 1
  • Ch. Schrader
    • 1
  1. 1.Federal Institute for Health Protection of Consumers and Veterinary MedicineBerlinFederal Republic of Germany
  2. 2.Department for Viral ZoonosisFederal Institute for Health Protection of Consumers and Veterinary MedicineBerlinFederal Republic of Germany

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