Characterization of a cowpox-like orthopox virus which had caused a lethal infection in man

  • C.-P. Czerny
  • C. Zeller-Lue
  • A. M. Eis-Hübinger
  • O.-R. Kaaden
  • H. Meyer


In August 1990 an orthopox virus (OPV) had been isolated from a severe case of a generalized infection with lethal outcome in an immunosup-pressed 18-year-old man. In this communication we present a detailed characterization of the causative virus strain. Based on distinct epitope configurations detected by various monoclonal antibodies the isolate could be differentiated from other OPV species and was classified as a cowpox virus (CP). This classification was confirmed by a species-specific PCR assay and by establishing physical maps for the restriction enzymes HindIII and XhoI. Based on serological data of neutralization assays, blocking-ELISAs and Western blotting analysis evidence is provided that the infection had beed acquired from a stray cat.


Vaccinia Virus Hyperimmune Serum Hyperimmune Seron Cowpox Virus Ectromelia Virus 
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Copyright information

© Springer-Verlag Wien 1997

Authors and Affiliations

  • C.-P. Czerny
    • 1
    • 2
    • 5
  • C. Zeller-Lue
    • 1
  • A. M. Eis-Hübinger
    • 3
  • O.-R. Kaaden
    • 1
    • 2
  • H. Meyer
    • 4
  1. 1.Institute for Medical Microbiology, Infectious and Epidemic DiseasesLudwig-Maximilians-UniversityMunichGermany
  2. 2.WHO Collaborating Centre for Collection and Evaluation of Data on Comparative Virology, Veterinary FacultyLudwig-Maximilians-UniversityMunichGermany
  3. 3.Institute for Medical Microbiology and ImmunologyUniversity of BonnBonnGermany
  4. 4.Institute of MicrobiologyFederal Armed Forces Medical AcademyMunichGermany
  5. 5.Institute für Medizinische MikrobiologieMünchenFederal Republic of Germany

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