Material and methods

  • Facundo Valverde


This atlas is based on the brain of the young postnatal mouse. This animal was chosen for several reasons: First, the brain of the mouse, smaller than that of the rat, can be impregnated in toto, especially at an early postnatal period, by using the Golgi method. Thus, reconstructions in any of the standard orientations can be made from one single brain while maintaining its three-dimensional representation. Second, the entire brain can be scaled to a convenient size without loss of information. Third, the probability that the majority of the brain structures are homogeneously stained is higher. The disadvantage is that young postnatal animals are still in the process of completing their development and not all structures and connections correspond to the adult state. Also, due to the unpredictability of the Golgi method, experience has made us painfully aware of the virtual impossibility of obtaining uniform impregnation of all structures in the same brain. However, for the purposes of this atlas, these disadvantages will not present any major obstacles; otherwise, if I had to wait for the ideal Golgi-stained brain, the production of this atlas would never have been possible.


Entire Brain Contiguous Slice Interhemispheric Fissure Axonal Arbor Anterior Olfactory Nucleus 


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Copyright information

© Springer-Verlag Wien 1998

Authors and Affiliations

  • Facundo Valverde
    • 1
  1. 1.Laboratorio de Neuroanatomía Comparada CSICInstituto CajalMadridSpain

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