Summary
A semicarbazide-sensitive amine oxidase (SSAO) (E.C.1.4.3.6) has been purified from pig heart. Western blot analysis showed that the enzyme reacts with a polyclonal antibody raised against homogeneous crystalline pig plasma benzylamine oxidase (BAO). A subunit molecular mass of 97 KDa obtained by SDS electrophoresis is identical to the plasma enzyme. The purification procedure consisted of sequential DEAE cellulose, octyl-Sepharose, Con A-Sepharose and hydroxyapatite columns. Two peaks of activity were obtained on octyl-Sepharose which were found to be kinetically and immunologically indistinguishable. The specific activity of the purified enzyme was 0.045 (imol/min/mg of protein at 37°C and the Km for benzylamine was estimated to be 63 μM. The enzyme was inhibited by carbonyl reagents such as semicarbazide but was insensitive to the effect of pargyline.
Research fellow of Europe (grant CHRXCT93-0256). Present address: Department of Biochemistry, Trinity College, Dublin, Ireland
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References
Buffoni F (1995) Semicarbazide-sensitive amine oxidases: some biochemical properties and general considerations. In: Yu PM, Tipton KF, Boulton AA (eds) Current neurochemical and pharmacological aspects of biogenic amines. Their function, oxidative deamination and inhibition. Elsevier, Amsterdam, pp 323–331 (Prog Brain Res 106)
Buffoni F, Blaschko H (1964) Benzylamine oxidase and histaminase: purification and crystallization of an enzyme from pig plasma. Proc Roy Soc B 161: 153–167
Buffoni F, Delia Corte L, Hope DB (1977) Immunofluorescence histochemistry of porcine tissue using antibodies to pig plasma amine oxidase. Proc Roy Soc B 195: 417–423
Buffoni F, Banchelli G, Bertocci B, Raimondi L (1989) Effect of pyridoxamine on semicarbazide-sensitive amine oxidase activity of rabbit lung and heart. J Pharm Pharmacol 41: 469–473
Buffoni F, Cambi S, Banchelli G, Ignesti G, Pirisino R, Raimondi L (1994) Semicarbazide-sensitive amine oxidase activity of guinea pig dorsal skin. J Neural Transm [Suppl] 41: 421–426
Callingham BA, Fowler CJ (1977) Monoamine oxidation in tissues of the developing chick. Br J Pharmacol 60: 306P
Clarke DE, Lyles GA, Callingham BA (1982) A comparison of cardiac clorgyline resistant amine oxidase and monoamine oxdidase. Biochem Pharmacol 31: 27–35
Coughtrie MWH, Burchell B, Leakey JEA, Hume R (1988) The inadequacy of perinatal glucuronidation immunoblot analysis of the developmental expression of individual UDP-glucuronyltransferase isoenzymes in rat and human liver microsomes. Mol Pharmacol 34: 729–735
Hysmith RM, Boor PJ (1988) Purification of benzylamine oxidase from cultured porcine aortic smooth muscle cells. Biochem Cell Biol 66: 821–829
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Leatherbarrow RJ (1987) Enzfitter. A non-linear regression data analysis program for IBM PC. Elsevier Biosoft
Lizcano JM, Balsa D, Tipton KF, Unzeta M (1990) Amine oxidase activities in bovine lung. J Neural Transm [Suppl] 32: 341–344
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Lyles GA, Callingham BA (1979) Selective influences of age and thyroid hormones on type A monoamine oxidase of the rat heart. J Pharm Pharmacol 31: 755–760
Lyles GA, Singh I (1985) Vascular smooth muscle cells: a major source of the semicarbazide-sensitive amine oxidase of the rat aorta. J Pharm Pharmacol 37: 637–643
Matsumoto T, Furuta T, Nimura Y (1982) Increased sensitivity of the fluorometric method of Snyder and Hendley for oxidase assay. Biochem Pharmacol 31: 2307–2309
Mehrabian ZB, Nalbandyan RM (1983) Benzylamine oxidase from brain microvessels. FEBS Lett 164: 89–92
Puliti M, Cambi S, Buffoni F (1996) Evidence of semicarbazide-sensitive amine oxidase activity in guinea pig tissues. Comp Biochem Physiol 115B: 159–165
Raimondi L, Pirisino R, Ignesti G, Capecchi S, Banchelli G, Buffoni F (1991) Semicarbazide-sensitive amine oxidase activity (SSAO) of rat epididymal white adipose tissue. Biochem Pharmacol 41: 467–470
Tabor H, Tabor CW, Rosenthal SM (1954) Purification of amine oxidase from beef plasma. J Biol Chem 208: 645–661
Tipnis UR, Tao M, Boor PJ (1992) Purification and characterization of semicarbazide-sensitive amine oxidase from porcine aorta. Cell Mol Biol 38: 575–584
Tiselius A, Hjerten S, Levin O (1956) Protein chromatography on calcium phosphate columns. Arch Biochem Biophys 65: 132–155
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 76: 4350–4354
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Dowling, T.G., Cambi, S., Buffoni, F. (1998). Semicarbazide-sensitive amine oxidase in pig heart. In: Finberg, J.P.M., Youdim, M.B.H., Riederer, P., Tipton, K.F. (eds) MAO — The Mother of all Amine Oxidases. Journal of Neural Transmission. Supplement, vol 52. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6499-0_26
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