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Studies on the time-dependent activation of microsomal semicarbazide-sensitive amine oxidase

  • J. M. Lizcano
  • A Fernández de Arriba
  • K. F. Tipton
  • M. Unzeta
Part of the Journal of Neural Transmission. Supplement book series (NEURAL SUPPL, volume 52)

Summary

The semicarbazide-sensitive amine oxidase (SSAO) from bovine lung microsomes was activated in a temperature- and time-dependent process. This behaviour was observed when the enzyme was preincubated at 25°C, 37°C and 50°C but not at 4°C. This activation was only observed when benzylamine was used as substrate but not when methylamine, histamine or 2phenylethylamine were used. The activation was independent of pH, ionic strength and the nature of the buffer used. At 37°C the specific activity had risen to a value that was about 7 times higher than that of the starting material after 120 min. This process affected only the maximum velocity of the reaction with the Km value remaining essentially unchanged. Treatment of SSAO with phospholipases and detergents did not affect this behaviour. Incubation of the enzyme with serine proteases, metal chelating agents, reducing agents or protease inhibitors, had no effect on the activation. The fact that both forms of the enzyme (activated and non-activated), showed the same Mr values on gel filtration chromatography excluded the possibility of an enzyme aggregation and/or degradation being involved in this process.

Keywords

Amine Oxidase Reactive Oxygen Intermediate Sodium Cholate Bovine Lung Preincubation Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barrand MA, Callingham BA (1982) New amine oxidases activities in brown adipose tissue of the rat: some properties and subcellular distribution. Biochem Pharmacol 31: 2177–2184PubMedCrossRefGoogle Scholar
  2. Crosbie A, Callingham B (1994) Semicarbazide sensitive amine oxidase in sheep plasma: interaction with some substrates and inhibitors. J Neural Transm [Suppl] 41:427–432Google Scholar
  3. Feng L, Xia Y, Garcia GE, Hwang D, Wilson CB (1995) Involvement of reactive oxygen intermediates in cyclooxygenase-2 expression induced by interleukin-1, tumor necrosis factor-alpha, and lipopolysaccharide. J Clin Invest 95: 1669–1675PubMedCrossRefGoogle Scholar
  4. Fowler CJ, Tipton KF (1981) Concentration dependence of the oxidation of tyramine by the two forms of rat liver mitochondrial monoamine oxidase. Biochem Pharmacol 30: 3329–3332PubMedCrossRefGoogle Scholar
  5. Hartree EP (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48: 422–427PubMedCrossRefGoogle Scholar
  6. Lizcano JM, Fernández de Arriba A, Lyles GA, Unzeta M (1994) Several aspects of the amine oxidation by semicarbazide-sensitive amine oxidase (SSAO) from in bovine lung. J Neural Transm [Suppl] 41: 415–420Google Scholar
  7. Meyer M, Schreck R, Baeuerle PA (1993) H2O2 and antioxidants have opposite effects on activation of NF-kappa B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor. EMBO J 12: 2005–2015PubMedGoogle Scholar
  8. Mu D, Janes SM, Smith AJ, Brown DE, Dooley DM, Klinman JP (1992) Tyrosine codon corresponds to topa quinone at the active site of copper amine oxidase. J Biol Chem 267: 7979–7982PubMedGoogle Scholar
  9. Precious E, Gunn CE, Lyles GA (1988) Deamination of methylamine by semicarbazide-sensitive amine oxidase in human umbilical artery and rat aorta. Biochem Pharmacol 37: 707–713PubMedCrossRefGoogle Scholar
  10. Schreck R, Rieber P, Baeuerle PA (1991) Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J 10: 2247–2258PubMedGoogle Scholar
  11. Tabor CW, Tabor H, Rosenthal SM (1954) Purification of amine oxidase from beef plasma. J Biol Chem 208: 644–661Google Scholar

Copyright information

© Springer-Verlag Wien 1998

Authors and Affiliations

  • J. M. Lizcano
    • 1
  • A Fernández de Arriba
    • 1
  • K. F. Tipton
    • 2
  • M. Unzeta
    • 1
    • 3
  1. 1.Department of Bioquimica i Biologia MolecularUniversitat Autonoma de BarcelonaBarcelona, BellaterraSpain
  2. 2.Biochemistry DepartmentTrinity CollegeDublinIreland
  3. 3.Departament de Bioquímica i de Biologia MolecularFacultad de MedicinaBellaterra (Barcelona)Spain

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