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Influence of maturation and ageing on the biotransformation of noradrenaline in the rat

  • J. T. Guimarães
  • M. Q. Paiva
  • D. Moura
  • S. Guimarães
Part of the Journal of Neural Transmission. Supplement book series (NEURAL SUPPL, volume 52)

Summary

The present investigation was undertaken to study the influence of maturation and ageing on the disposition of noradrenaline by the aorta, heart (ventricle), liver and kidney of the rat. Slices of these tissues taken from rats aged less than 18 h, 2.5–3 months or 18–24 months were incubated with 0.1 μmol.l-1 3H-amine during 30min. At the end of this period, the accumulation of the intact amine in the tissue, as well as the 3Hmetabolites formed (3,4-dihydroxyphenylethylglycol, 3,4-dihydroxymandelic acid, normetanephrine and O-methylated deaminated metabolites) were determined by scintillation counting. The results obtained show that in the rat: 1) at any age, noradrenaline is preferentially deaminated; 2) while the capacity of the sympathetic nerve terminals in accumulating noradrenaline is rather well developed at birth, the metabolic system for its degradation is still immature; 3) aldehyde dehydrogenase activity or that of its co-factor (or both) of the heart is apparently missing at birth; 4) removal of noradrenaline by the liver and the kidney did not change with ageing, while that by the aorta decreased and that by the heart increased.

Keywords

Noradrenaline Content Sympathetic Nerve Terminal Total Metabolite Aldehyde Dehydrogenase Activity Extraneuronal Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Blatchford D, Holzbauer M, Grahame-Smith DG, Youdim MBH (1976) Ontogenesis of enzyme systems deaminating different monoamines. Br J Pharmacol 57: 279–293CrossRefPubMedGoogle Scholar
  2. Dahlström A, Häggendal J (1967) Studies on the transport and lifespan of amine storage granules in the adrenergic neuron system of the rabbit sciatic nerves. Acta Phvsiol Scand 69: 153–157CrossRefGoogle Scholar
  3. Docherty JR (1990) Cardiovascular responses in ageing: a review. Pharmacol Rev 42: 103–125PubMedGoogle Scholar
  4. Eisenhofer G (1994) Plasma normetanephrine for examination of extraneuronal uptake and metabolism of noradrenaline in rats. Naunyn Schmiedebergs Arch Pharmacol 349: 259–269CrossRefPubMedGoogle Scholar
  5. Eisenhofer G, Finberg JPM (1994) Different metabolism of norepinephrine and epinephrine by catechol-O-methyltransferase and monoamine oxidase on rats. J Pharmacol Exp Ther 268: 1242–1251PubMedGoogle Scholar
  6. Fiebig ER, Trendelenburg U (1978) The neuronal and extraneuronal uptake and metabolism of 3H-(-)-noradrenaline in the perfused rat heart. Naunyn Schmiedebergs Arch Pharmacol 303: 21–35CrossRefPubMedGoogle Scholar
  7. Garrett J, Branco D (1977) Uptake and metabolism of noradrenaline by the mesenteric arteries of the dog. Blood Vessels 14: 43–54PubMedGoogle Scholar
  8. Graefe K-H, Stefano F, Langer SZ (1973) Preferential metabolism of (-)-3H-norepinephrine through deaminated glycol in the rat vas deferens. Biochem Pharmacol 22: 1147–1160CrossRefGoogle Scholar
  9. Guimarães S (1975) Further study of the adrenoceptors of the saphenous vein of the dog: influence of factors which interfere with the concentration of agonists at the receptor level. Eur J Pharmacol 34: 9–19CrossRefPubMedGoogle Scholar
  10. Guimarães S, Paiva MQ (1977) The role played by the extraneuronal system in the disposition of noradrenaline and adrenaline in vessels. Naunyn Schmiedebergs Arch Pharmacol 296: 279–287CrossRefPubMedGoogle Scholar
  11. Guimarães S, Brandão F, Paiva MQ (1978) A study of the adrenoceptor-mediated feedback mechanism by using adrenaline as a false transmitter. Naunyn Schmiedebergs Arch Pharmacol 305: 185–188CrossRefPubMedGoogle Scholar
  12. Guimarães S, Paiva MQ, Moura D, Vaz-da-Silva M (1991) Presynaptic α-autoadrenoceptors on peripheral noradrenergic neurones of newborn rabbits and dogs. In: Langer SZ, Galzin AM, Costentin J (eds) Presynaptic receptors and neuronal transporters. Pergamon Press, Oxford, pp 11–14 (Advances in the Biosciences 8)Google Scholar
  13. Laduron P, Belpaire F (1968) Transport of noradrenaline and dopamine-ß-hydroxilase in sympathetic nerves. Life Sci 7: 1–7CrossRefPubMedGoogle Scholar
  14. Martel F, Azevedo I, Osswald W (1993) Uptake and metabolism of 3H-adrenaline and 3H-noradrenaline by isolated hepatocytes and liver slices of the rat. Naunyn Schmiedebergs Arch Pharmacol 348: 450–457CrossRefPubMedGoogle Scholar
  15. Moura D, Vaz-da-Silva MJ, Azevedo I, Brandäo F, Guimarães S (1993) Release and disposition of 3H-noradrenaline in the saphenous vein of neonate and adult dogs. Naunyn Schmiedebergs Arch Pharmacol 347: 186–191CrossRefPubMedGoogle Scholar
  16. Paiva MQ, Guimarães S (1978) A comparative study of the uptake and metabolism of noradrenaline and adrenaline by the isolated saphenous vein of the dog. Naunyn Schmiedebergs Arch Pharmacol 303: 221–228PubMedGoogle Scholar
  17. Steinberg P, Acevedo C, Masana MI, Rubio MC (1988) Uptake and metabolism of 3H-(±)-noradrenaline in the isolated perfused rat liver. Naunyn Schmiedebergs Arch Pharmacol 337: 392–396CrossRefPubMedGoogle Scholar
  18. Su C, Bevan JA, Assali NS, Brinkman CR (1977) Development of neuroeffector mechanisms in the carotid artery of the fetal lamb. Blood Vessels 14: 12–24PubMedGoogle Scholar
  19. Trendelenburg U, Stefano E, Grohmann M (1983) The isotope effect of tritium in 3H-noradrenaline. Naunyn Schmiedebergs Arch Pharmacol 323: 128–140CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 1998

Authors and Affiliations

  • J. T. Guimarães
    • 2
  • M. Q. Paiva
    • 1
  • D. Moura
    • 1
  • S. Guimarães
    • 1
    • 1
  1. 1.Institute of Pharmacology and Therapeutics, Faculty of MedicinePortoPortugal
  2. 2.Department of Biochemistry, Faculty of MedicinePortoPortugal

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