Advertisement

Modulation of glutamate neurotoxicity in the transformed cell culture by monoamine oxidase inhibitors, clorgyline and deprenyl

  • O. Yu. Abakumova
  • O. V. Podobed
  • T. A. Tsvetkova
  • I. V. Yakusheva
  • T. A. Moskvitina
  • L. I. Kondakova
  • D. G. Navasardyantz
  • A. E. Medvedev
Part of the Journal of Neural Transmission. Supplement book series (NEURAL SUPPL, volume 52)

Summary

Addition of 30 mM glutamate to the culture medium decreased growth of rat glioma C6 cells accompanied by a decrease of DNA synthesis and an increase of lactate dehydrogenase (LDH) detected in the conditioned medium. The presence of 1μM deprenyl attenuated the glutamate effect on cell growth only during the first 24–48 h incubation and had a minor influence on the glutamate-induced decrease of DNA synthesis. Clorgyline (lμM) potentiated glutamate-induced DNA synthesis during the first 24 h incubation without significant influence on the cell growth. Deprenyl slightly attenuated the glutamate-induced LDH increase during 24 h incubation but potentiated the glutamate effect at 96 h. Clorgyline decreased the glutamate influence at 24 h and especially 96 h. All these effects were observed in the absence of exogenous monoamines in the culture medium.

These results suggest that in transformed cells monoamine oxidase (MAO) inhibitors may influence processes of cell death via MAO-independent mechanisms.

Keywords

Monoamine Oxidase Monoamine Oxidase Inhibitor Biomedical Chemistry Glutamate Neurotoxicity Parkinson Study Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Carillo MC, Kanai S, Nokubu M, Kitani K (1991) (-)Deprenyl induces activities of both superoxide dismutase and catalase but not glutathione peroxidase in the striatum of young male rats. Life Sci 48: 517–521CrossRefGoogle Scholar
  2. Clow A, Hussain T, Glover V, Sandler M, Dexter DT, Walker M (1991) (-)Deprenyl can induce soluble superoxide dismutase in rat striata. J Neural Transm 86: 77–80CrossRefGoogle Scholar
  3. Keung W, Sieber E, Eppenberger U (1989) Quantitation of cells cultured on 96 well plates. Anal Biochem 182: 16–19CrossRefGoogle Scholar
  4. Knoll J (1988) The striatal dopamine dependency of life span in male rats. Longevity study with (-)deprenyl. Mech Ageing Dev 46: 237–262PubMedCrossRefGoogle Scholar
  5. Knoll J, Dallo J, Yen TT (1989) Striatal dopamine, sexual activity and life span. Longevity of rats treated with (-)deprenyl. Life Sci 45: 525–531PubMedCrossRefGoogle Scholar
  6. Medvedev AE, Fuchs BB, Rakhmilevich AI (1990) A study of the action of immunosupressive factors from tumor cells on lymphocytes and macrophages in vitro and on the graft-versus-host reaction in mice. Biomed Sci 1: 261–266PubMedGoogle Scholar
  7. Medvedev AE, Kirkel AZ, Kamyshanskaya NS, Axenova LN, Moskvitina TA, Gorkin VZ, Andreeva NI, Mashkovsky MD (1994) Monoamine oxidase inhibition by novel antidepressant tetrindole. Biochem Pharmacol 47: 303–308PubMedCrossRefGoogle Scholar
  8. Murphy TH, Miyamoto M, Sastre A, Schnaar R, Coyle JT (1989) Glutamate toxicity in a neuronal cell line involves inhibition of cysteine transport leading to oxidative stress. Neuron 2: 1547–1558PubMedCrossRefGoogle Scholar
  9. Parkinson Study Group (1993) Effects of tocopherol and deprenyl on the progression of disability in early Parkinson’s disease. N Engl J Med 328: 176–183CrossRefGoogle Scholar
  10. Ratan RR, Murphy TH, Baraban JM (1994) Oxidative stress induced apoptosis in embryotic cortical neurons. J Neurochem 62: 376–379PubMedCrossRefGoogle Scholar
  11. Tatton WG, Seniuk NA, Ju WYH, Ansari KS (1994) Reduction of nerve cell death by deprenyl without monoamine oxidase inhibition. In: Lieberman A, Olanow O, Youdim MBH, Tipton KF (eds) Monoamine oxidase inhibitors in neurological diseases. Raven Press, New York, pp 217–248Google Scholar
  12. Tetrud VW, Lanston JW (1989) The effect of deprenyl (selegiline) on the natural history of Parkinson’s disease. Science (Wahsington DC) 245: 519–522CrossRefGoogle Scholar
  13. Tipton KF, Youdim MBH (1976) Assay of monoamine oxidase. In: Wolstenholm GEW, Knight J (eds) Monoamine oxidase and its inhibition. Elsevier, Amsterdam, pp 393–403Google Scholar

Copyright information

© Springer-Verlag Wien 1998

Authors and Affiliations

  • O. Yu. Abakumova
    • 1
    • 2
  • O. V. Podobed
    • 1
  • T. A. Tsvetkova
    • 1
  • I. V. Yakusheva
    • 1
  • T. A. Moskvitina
    • 1
  • L. I. Kondakova
    • 1
  • D. G. Navasardyantz
    • 1
  • A. E. Medvedev
    • 1
  1. 1.Institute of Biomedical ChemistryAcademy of Medical SciencesMoscowRussia
  2. 2.Institute of Biomedical ChemistryRussian Academy of Medical SciencesMoscowRussia

Personalised recommendations