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Life beyond eradication: veterinary viruses in basic science

  • L. W. Enquist
Chapter
Part of the Archives of Virology. Supplementa book series (ARCHIVES SUPPL, volume 15)

Summary

To some, the focus of research in virology entails the search for solutions of practical problems. By definition then, attention is limited to those viruses that cause disease or to exploitation of some aspect of virology to a practical end (e.g., antiviral drugs or vaccines). Once a disease is cured, or the agent eradicated, it is time to move on to something else. To others, virology offers the opportunity to study fundamental problems in biology. Work on these problems may offer no obvious practical justification; it is an affliction of the terminally curious, perhaps with the outside hope that something “useful” will come of it. To do this so-called “basic science”, one must find the most tractable system to solve the problem, not the system that has “relevance” to disease. I have found that veterinary viruses offer a variety of opportunities to study relevant problems at the fundamental level. To illustrate this point, I describe some recent experiments in my laboratory using pseudorabies virus (PRV), a swine herpesvirus.

Keywords

Enhance Green Fluorescent Protein Pseudorabies Virus Live Vaccine Strain Dorsal Motor Vagal Nucleus Green Fluorescent Protein Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Babic N, Mettenleiter TC, Flamand A, Ugolini G (1993) Role of essential glycoproteins gII and gp50 in transneuronal transfer of pseudorabies virus from the hypoglossal nerves of mice. J Virol 67: 4421–4426PubMedGoogle Scholar
  2. 2.
    Babic N, Klupp B, Brack A, Mettenleiter TC, Ugolini G, Flamand A (1996) Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation. Virology 219: 279–284PubMedCrossRefGoogle Scholar
  3. 3.
    Banfield BW, Yap GS, Knapp AC, Enquist LW (1998) A chicken embryo eye model for the analysis of alphaherpesvirus neuronal spread and virulence. J Virol 72: 4580–4588PubMedGoogle Scholar
  4. 4.
    Bartha A (1961) Experimental reduction of virulence of Aujeszky’s disease virus. Magy Allatorv Lapja 16: 42–45Google Scholar
  5. 5.
    Brideau AD, Banfield BW, Enquist LW (1998) The Us9 gene product of pseudorabies virus, an alpha herpesvirus, is a phosphorylated, tail anchored type II membrane protein. J Virol 72: 4 560–4 570Google Scholar
  6. 6.
    Card JP, Levitt P, Enquist LW (1998) Different patterns of neuronal infection after intracerebral injection of two strains of pseudorabies virus. J Virol 72: 4434–4441PubMedGoogle Scholar
  7. 7.
    Card JP, Rinaman L, Schwaber JS, Miselis RR, Whealy ME, Robbins AK, Enquist LW (1990) Neurotropic properties of pseudorabies virus: uptake and transneuronal passage in the rat central nervous system. J Neurosci 10: 1 974–1 994Google Scholar
  8. 8.
    Card JP, Whealy ME, Robbins AK, Moore RY, Enquist LW (1991) Two α-herpesvirus strains are transported differentially in the rodent visual system. Neuron 6: 957–969PubMedCrossRefGoogle Scholar
  9. 9.
    Cohen J (1998) Infection of cells with varicella-zoster virus down-regulates surface expression of class I major histocompatibility complex antigens. Infect Dis 155: 1 390–1393Google Scholar
  10. 10.
    Cook ML, Stevens JG (1973) Pathogenesis of herpetic neuritis and ganglionitis in mice: evidence for intra-axonal transport of infection. Infect Immun 7: 272–288PubMedGoogle Scholar
  11. 11.
    Davison AJ, Scott JE (1986) The complete DNA sequence of varicella-zoster virus. J Gen Virol 67: 1759–1816PubMedCrossRefGoogle Scholar
  12. 12.
    Dingwell KS, Brunetti CR, Hendricks RL, Tang Q, Tang M, Rainbow AJ, Johnson DC (1994) Herpes simplex virus glycoproteins E and I facilitate cell-to-cell spread in vivo and across junctions of cultured cells. J Virol 68: 834–845PubMedGoogle Scholar
  13. 13.
    Dingwell KS, Doering LC, Johnson DC (1995) Glycoproteins E and I facilitate neuron-to-neuron spread of herpes simplex virus. J Virol 69: 7 087–7 098Google Scholar
  14. 14.
    Dolivo M (1980) A neurobiological approach to neurotropic viruses. Trends Neurosci 3: 149–152CrossRefGoogle Scholar
  15. 15.
    Dolivo M, Beretta E, Bonifas V, Foroglou C (1978) Ultrastructure and function in sympathetic ganglia isolated from rats infected with pseudorabies virus. Brain Res 140: 111–123PubMedCrossRefGoogle Scholar
  16. 16.
    Dolivo M, Honegger P, George C, Kiraly M, Bommeli W (1979) Enzymatic activity, ultrastructure and function in ganglia infected with a neurotropic virus. In: Cuenod M, Kreutzberg GW, Bloom FE (eds) Development and chemical specificity of neurons, Vol 51. Elsevier/North-Holland Biomedicai Press, Amsterdam, pp 51–57CrossRefGoogle Scholar
  17. 17.
    Enquist LW, Card JP (1996) Pseudorabies virus: a tool for tracing neuronal connections. In: Lowenstein R, Enquist LW (eds) Protocols for gene transfer in neuroscience: towards gene therapy of neurological disorders. J Wiley, Chichester, pp 333–348Google Scholar
  18. 18.
    Enquist LW, Husak PJ, Banfield BW, Smith GA (1998) Infection and spread of alpha-herpesviruses in the nervous system. Adv Virus Res 58: 237–347CrossRefGoogle Scholar
  19. 19.
    Favoreel HW, Nauwynck JJ, van Oostveldt P, Mettenleiter TC, Pensaert MB (1997) Antibody-induced and cytoskeleton-mediated redistribution and shedding of viral glycoproteins, expressed on pseudorabies virus-infected cells. J Virol 71: 8254–8 261PubMedGoogle Scholar
  20. 20.
    Frame MC, McGeoch DJ, Rixon FJ, Orr AC, Marsden HS (1986) The 10 K virion phophoprotein encoded by gene Us9 from herpes simplex virus type I. Virology 150: 321–332PubMedCrossRefGoogle Scholar
  21. 21.
    Goodpasture EW, Teague O (1923) Transmission of the virus of herpes fibrils along nerves in experimentally infected rabbits. J Med Res 44: 139–184PubMedGoogle Scholar
  22. 22.
    Goodpasture EW (1925) The axis-cylinders of peripheral nerves as portals of entry to the central nervous system for the virus of herpes simplex in experimentally infected rabbits. Am J Pathol 1: 11–33PubMedGoogle Scholar
  23. 23.
    Hengel H, Koszinowski UH (1997) Interference with antigen processing by viruses. Curr Opin Immunol 9: 470–476PubMedCrossRefGoogle Scholar
  24. 24.
    Jacobs L (1994) Glycoprotein E of pseudorabies virus and homologous proteins in other alphaherpesvirinae. Arch Virol 137: 209–228PubMedCrossRefGoogle Scholar
  25. 25.
    Jansen ASP, Nguyen XV, Karpitskiy V, Mettenleiter TC, Loewy AD (1995) Central command neurons of the sympathetic nervous system: basis of the fight-or-flight response. Science 270: 644–646PubMedCrossRefGoogle Scholar
  26. 26.
    Jons A, Mettenleiter TC (1997) Green fluorescent protein expressed by recombinant pseudorabies virus as an in vivo marker for viral replication. J Virol Methods 66: 283–292PubMedCrossRefGoogle Scholar
  27. 27.
    Kimman TG, Bionchi ATJ, de Bruin TGM, Mulder WAM, Priem J, Voermans JM (1995) Interaction of pseudorabies virus with immortalized porcine B cells: influence on surface class I and II major histocompatibility and immunoglobulin M expression. Vet Immunol Immunopath 45: 253–263CrossRefGoogle Scholar
  28. 28.
    Kovacs SzF, Mettenleiter TC (1991) Firefly luciferase as a marker for herpesvirus (pseudorabies virus) replication in vitro and in vivo. J Gen Virol 72: 2 999–3 008Google Scholar
  29. 29.
    Kristensson K (1970) Morphological studies of the neural spread of herpes simplex virus to the central nervous system. Acta Neuropathol 16: 54–63PubMedCrossRefGoogle Scholar
  30. 30.
    Kristensson K, Ghetti B, Wisniewski HM (1974) Study on the propagation of herpes simplex virus (type 2) into the brain after intraocular injection. Brain Res 69: 189–201PubMedCrossRefGoogle Scholar
  31. 31.
    Kristensson K, Lycke E, Sjostrand J (1971 ) Spread of herpes simplex virus in peripheral nerves. Acta Neuropathol 17: 44–53PubMedCrossRefGoogle Scholar
  32. 32.
    (Deleted in proof)Google Scholar
  33. 33.
    Kristensson K, Nennesmo I, Persson L, Lycke E (1982) Neuron to neuron transmission of herpes simplex virus: transport of virus from skin to brainstem nuclei. J Neurol Sci 54: 149–156PubMedCrossRefGoogle Scholar
  34. 34.
    Kristensson K, Vahlne A, Persson LA, Lycke E (1978) Neural spread of herpes simplex virus types 1 and 2 in mice after corneal or subcutaneous (footpad) inoculation. J Neurol Sci 35: 331–340PubMedCrossRefGoogle Scholar
  35. 35.
    Kritas SK, Pensaert MB, Mettenleiter TC (1994) Role of envelope glycoproteins gI, gp63 and gIII in the invasion and spread of Aujeszky’s disease virus in the olfactory nervous pathway of the pig. J Gen Virol 75: 2 319–2 327CrossRefGoogle Scholar
  36. 36.
    Kritas SK, Nauwynck HJ, Pensaert MB (1995) Dissemination of wild type and gC-, gE-and gl-deleted mutants of Aujeszky’s disease virus in the maxillary nerve and trigeminal ganglion of pigs after intranasal inoculation. J Gen Virol 76: 2 063–2 066CrossRefGoogle Scholar
  37. 37.
    Kuypers HGJM, Ugolini G (1990) Viruses as transneuronal tracers. Trends Neurosci 13: 71–75PubMedCrossRefGoogle Scholar
  38. 38.
    Loewy AD, Bridgman PC, Mettenleiter TC (1991) Beta-galactosidase expressing recombinant pseudorabies virus for light and electron microscopic study of transneu-ronally labeled CNS neurons. Brain Res 555: 346–352PubMedCrossRefGoogle Scholar
  39. 39.
    Loewy AD (1995) Pseudorabies virus: a tranneuronal tracer for neuroanatomical studies. In: Kaplitt MG, Loewy AD (eds) Viral vectors. Gene therapy and neuroscience applications. Academic Press, San Diego, pp 349–366Google Scholar
  40. 40.
    Lowenstein PR, Enquist LW (1995) Protocols for gene transfer in neuroscience; towards gene therapy of neurological disorders. J Wiley, New YorkGoogle Scholar
  41. 41.
    Mellencamp MW, O’Brien PCM, Stevenson JR (1991) Pseudorabies virus-induced suppression of major histocompatibility complex class I antigen expression. J Virol 65: 3 365–3 368Google Scholar
  42. 42.
    Messerle M, Crnkovic I, Hammerschmidt W, Ziegler H, Koszinowski UH (1997) Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. Proc Natl Acad Sci USA 94: 14759–14763PubMedCrossRefGoogle Scholar
  43. 43.
    Mettenleiter TC (1994) Pseudorabies (Aujeszky’s Disease) virus: state of the art. Acta Vet Hung 42: 153–177PubMedGoogle Scholar
  44. 44.
    Mettenleiter TC, Rauh I (1990) A glycoprotein gX-β-galactosidase fusion gene as in-sertional marker for rapid identification of pseudorabies virus mutants. J Virol Methods 30: 55–66PubMedCrossRefGoogle Scholar
  45. 45.
    Montgomery RI, Warner MS, Lum BJ, Spear PG (1996) Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell 87: 427–436PubMedCrossRefGoogle Scholar
  46. 46.
    Mulder W, Pol J, Kimman T, Kok G, Priem J, Peeters B (1996). Glycoprotein D-negative pseudorabies virus can spread transneuronally via direct neuron-to-neuron transmission in its natural host, the pig, but not after additional inactivation of gE or gI. J Virol 70: 2191–2200PubMedGoogle Scholar
  47. 47.
    Nataraj C, Eidmann S, Hariharan MJ, Sur JH, Perry GA, Srikumaran S (1997) Bovine herpesvirus 1 downregulates the expression of bovine MHC class I molecules. Viral Immunol 10: 21–34PubMedCrossRefGoogle Scholar
  48. 48.
    Olson JK, Grose C (1997) Endocytosis and recycling of varicella-zoster virus Fc receptor glycoprotein gE: internalisation mediated by a YXXL motif in the cytoplasmic tail. J Virol 71: 4042–4054PubMedGoogle Scholar
  49. 49.
    Peeters B, Pol J, Gielkens A, Moormann R (1993) Envelope glycoprotein gp50, of pseudorabies virus is essential for virus entry but is not required for viral spread in mice. J Virol 67: 170–177PubMedGoogle Scholar
  50. 50.
    Rauh I, Mettenleiter TC (1991) Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration. J Virol 65: 5 348–56Google Scholar
  51. 51.
    Roizman B, Sears E (1996) Herpes simplex viruses and their replication. In: Fields BN, Knipe DM, Howley PM (eds) Fundamental virology. Lippincott-Raven, Philadelphia New York, pp 1043–1107Google Scholar
  52. 52.
    Sabin AB (1938) Progression of different nasally instilled viruses along different nervous pathways in the same host. Proc Soc Exp Biol Med 38: 270–275Google Scholar
  53. 53.
    Schmidt J, Klupp BG, Karger A, Mettenleiter TC (1997) Adaptability in her-pesviruses: glycoprotein D-independent infectivity of pseudorabies virus. J Virol 71: 17–24PubMedGoogle Scholar
  54. 54.
    Standish A, Enquist LW, Miselis RR, Schwaber JS (1995) Dendritic morphology of cardiac related medullary neurons defined by circuit-specific infection by a recombinant pseudorabies virus expressing beta-galactosidase. J Neurovirol 1: 359–368PubMedCrossRefGoogle Scholar
  55. 55.
    Tirabassi RS, Townley RA, Eldridge MG, Enquist LW (1997) Characterization of Pseudorabies virus mutants expressing carboxy-terminal truncations of gE: evidence for envelope incorporation, virulence, and neurotropism domains. J Virol 71: 6455–6464PubMedGoogle Scholar
  56. 56.
    Tirabassi RS, Enquist LW (1998) The role of envelope protein gE endocytosis in the pseudorabies virus life cycle. J Virol 72: 4571–4579PubMedGoogle Scholar
  57. 57.
    Ugolini G (1995) Transneuronal tracing with alpha-herpesviruses: a review of the methodology. In: Kaplitt MG, Loewy AD (eds) Viral vectors. Gene therapy and neuroscience applications. Academic Press, San Diego, pp 293–317Google Scholar
  58. 58.
    Whitley RJ, Schlitt M (1991) Encephalitis caused by herpesviruses including B virus. In: Scheid WM, Whitley RJ, Durack DT (eds) Infections of the central nervous system. Raven Press, New York, pp 41–86Google Scholar
  59. 59.
    Wittmann G (1989) Herpesvirus diseases of cattle, horses and pigs. Kluwer, Boston Dordrecht LondonCrossRefGoogle Scholar
  60. 60.
    Whealy ME, Card JP, Robbins AK, Dubin JR, Rziha H-J, Enquist LW (1993) Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins. J Virol 67: 3 786–3 797Google Scholar
  61. 61.
    Zemanick MC, Strick PL, Dix RD (1991 ) Direction of transneuronal transport of herpes simplex virus 1 in the primate motor system is strain-dependent. Proc Natl Acad Sci USA 88: 8048–8051PubMedCrossRefGoogle Scholar
  62. 62.
    Zhu Z, Hao Y, Gershon MD, Ambron RT, Gershon AA (1996) Targeting of glycoprotein I(gE) of varicella-zoster virus to the trans-Golgi network by an AYRV sequence and an acidic amino acid-rich patch in the cytosolic domain of the molecule. J Virol 70: 6563–6575PubMedGoogle Scholar
  63. 63.
    Tirabassi RS, Enquist LW (1999) Mutation of the YXXL endocytosis motif in the cyto-plasmic tail of pseudorabies virus gE. J Virol 73: 2717–2728PubMedGoogle Scholar
  64. 64.
    Yang M, Card JP, Tirabassi RS, Miselis RR, Enquist LW (1999) Retrograde, transneu-ronal spread of pseudorabies virus in defined neuronal circuitry of the rat brain is facilitated by gE mutations that reduce virulence. J Virol 73: 4 350–4 359Google Scholar
  65. 65.
    Brideau A, del Rio T, Wolffe EJ, Enquist LW (1999) Intracellular trafficking and localization of the pseudorabies virus Us9 Type II envelope protein to host and viral membranes. J Virol 73: 4372–4384PubMedGoogle Scholar
  66. 66.
    Sparks-Thissen R, Enquist LW (1999) Differential regulation Dk and Kk MHC class I proteins on the cell surface after infection of murine cells by pseudorabies virus. J Virol 73: 5 748–5 756Google Scholar
  67. 67.
    Smith GA, Enquist LW (1999) Construction and transposon mutagenesis in E. coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus. J Virol (in press)Google Scholar

Copyright information

© Springer-Verlag Wien 1999

Authors and Affiliations

  • L. W. Enquist
    • 1
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA

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