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A single amino acid alteration in murine PrP dramatically alters TSE incubation time

  • J. C. Manson
  • R. Barron
  • E. Jamieson
  • H. Baybutt
  • N. Tuzi
  • I. McConnell
  • D. Melton
  • J. Hope
  • C. Bostock
Chapter
Part of the Archives of Virology. Supplementa book series (ARCHIVES SUPPL, volume 16)

Summary

In order to investigate mutations linked to human TSEs, we have used the technique of gene targeting to introduce specific mutations into the endogenous murine PrP gene which resulted in a P101L substitution (Prnp a101L ) in the murine PrP gene. This mutation is equivalent to the 102L mutation in the human PrP gene which is associated with Gerstmann-Sträussler syndrome. Since the mutated gene is in the correct chromosomal location and control of the mutant gene expression is identical to that of the wild type murine PrP gene, the precise effect of the 101L mutation in the uninfected and TSE infected mouse can be investigated in this transgenic model. Mice homozygous for this mutation (101LL) while showing no spontaneous TSE disease were more susceptible to TSE disease than wild type mice following inoculation with GSS infectivity. Disease was transmitted from these mice to mice both with and without the Prnp a101L allele. The 101L mutation does not therefore produce spontaneous genetic disease in mice but does dramatically alter incubation periods following TSE infection. Additionally, a rapid TSE transmission was demonstrated associated with extremely low amounts of PrPSc.

Keywords

Prion Protein Bovine Spongiform Encephalopathy Fatal Familial Insomnia 102L Mutation 10ILL Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Wien 2000

Authors and Affiliations

  • J. C. Manson
    • 1
  • R. Barron
    • 1
  • E. Jamieson
    • 1
  • H. Baybutt
    • 1
  • N. Tuzi
    • 1
  • I. McConnell
    • 1
  • D. Melton
    • 2
  • J. Hope
    • 3
  • C. Bostock
    • 3
  1. 1.BBSRC Neuropathogenesis UnitInstitute for Animal HealthEdinburghScotland, UK
  2. 2.Institute of Cell and Molecular BiologyEdinburgh UniversityEdinburghScotland, UK
  3. 3.Compton LaboratoryInstitute for Animal HealthNewbury, BerkshireUK

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