Inhibition of formation of protease-resistant prion protein by Trypan Blue, Sirius Red and other Congo Red analogs
Five compounds related to Congo Red were found to inhibit generation of protease-resistant prion protein in a cell-free system. In this assay Trypan Blue, Evans Blue, Sirius Red F3B, Primuline and Thioflavin-S were all more inhibitory than Congo Red itself. In scrapie-infected mouse neuroblastoma cells one compound, Sirius Red F3B, was capable of blocking the formation of protease-resistant prion protein to a similar extent as Congo Red; however, the other four compounds were less effective. Some of these compounds should be considered for testing in TSE disease models in live animals.
KeywordsTrypan Blue Prion Protein Prion Disease Bovine Spongiform Encephalopathy Evans Blue
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