Varicella-zoster virus with a lost gE epitope: evidence for immunological pressure by the human antibody response

  • J. A. Padilla
  • C. Grose
Conference paper


The varicella-zoster virus (VZV) genome contains about 70 open reading frames (ORF). ORF 68 codes for glycoprotein gE, formerly called gp1, which is the predominant VZV glycoprotein; gE is a typical type 1 transmembrane protein with 623 amino acids. Recently, a variant virus was discovered which has a mutation in gE codon 150; this mutation converts an aspartic acid into an asparagine residue.


Herpes Zoster Cell Epitope Variant Virus Antibody Dependent Cellular Cytotoxicity Varicella Vaccine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Arvin AM (1996) Varicella-zoster virus. In: Fields BN, Knipe DN, Howley PM (eds) Virology, 3rd ed. Lippincott-Raven, Philadelphia, pp 2547–2585Google Scholar
  2. 2.
    Arvin AM, Kinney-Thomas E, Shriver K, Grose C, Koropchak CM, Scanton E, Wittek AE, Diaz PS (1986) Immunity to varicella-zoster virus glycoproteins gpI (gp 90/58) and gpIII (gp 118), and to a nonglycosylated protein, p170. J Immunol 137: 1346–1351PubMedGoogle Scholar
  3. 3.
    Bergen RE, Sharp M, Sanchez A, Judd AK, Arvin AM (1991) Human T-cells recognize multiple epitopes of an immediate early tegument protein (1E62) and glycoprotein I of varicella zoster virus. Viral Immunol 4: 151–166PubMedCrossRefGoogle Scholar
  4. 4.
    Davison AJ, Scott JE (1986) The complete DNA sequence of varicella-zoster virus. J Gen Virol 67: 1759–1816PubMedCrossRefGoogle Scholar
  5. 5.
    Gershon AA, Steinberg SP, The NIAID Varicella Vaccine Collaborative Study Group (1989) Persistence of immunity to varicella in children with leukemia immunized with live attenuated varicella vaccine. N Engl J Med 320: 892–897CrossRefGoogle Scholar
  6. 6.
    Grose C (1981) Variation on a theme by Fenner: the pathogenesis of chickenpox. Pedi­atrics 68: 735–737Google Scholar
  7. 7.
    Grose C (1983) Zoster in children with cancer: radioimmune precipitation profiles of sera before and after illness. J Infect Dis 147: 47–55PubMedCrossRefGoogle Scholar
  8. 8.
    Grose C (1990) Glycoproteins encoded by varicella-zoster virus: biosynthesis, phospho­rylation, and intracellular trafficking. Annu Rev Microbiol 44: 59–80PubMedCrossRefGoogle Scholar
  9. 9.
    Grose C (1999) Varicella-zoster virus: less immutable than once thought. Pediatrics 103: 1027–1028PubMedCrossRefGoogle Scholar
  10. 10.
    Grose C, Edwards DP, Freidrichs WE, Weigle KA, McGuire WL (1983) Monoclonal antibodies against three major glycoproteins of varicella-zoster virus. Infect Immun 40: 381–388PubMedGoogle Scholar
  11. 11.
    Ito M, Ihara T, Grose C, Starr S (1985) Human leukocytes kill varicella-zoster virus infected fibroblasts in the presence of murine monoclonal antibodies of virus-specific glycoproteins. J Virol 54: 98–105PubMedGoogle Scholar
  12. 12.
    Kinchington PR, Turse SE (1995) Molecular basis for a geographic variation of varicella­zoster virus recognized by a peptide antibody. Neurology 45 [Suppl 8]: S13—S14Google Scholar
  13. 13.
    LaRussa PS, Gershon AA, Steinberg SP, Chartrand SA (1990) Antibodies to varicella­zoster virus glycoproteins I, II, and III in leukemic and healthy children. J Infect Dis 162: 627–633PubMedCrossRefGoogle Scholar
  14. 14.
    McGeoch DJ, Cook S (1994) Molecular phylogeny of the Alphaherpesvirinae subfamily and a proposed evolutionary timescale. J Mol Biol 238: 9–22PubMedCrossRefGoogle Scholar
  15. 15.
    McGeoch DJ, Cook S, Dolan A, Jamieson FE, Telford EAR (1995) Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses. J Mol Biol 247: 443–458PubMedCrossRefGoogle Scholar
  16. 16.
    McGeoch DJ, Davison AJ (1999) The molecular evolutionary history of the her­pesviruses. In: Domingo E, Webster R, Holland J (eds) Origin and evolution of viruses. Academic Press, New York, pp 441–465CrossRefGoogle Scholar
  17. 17.
    Padilla JA, Uno F, Yamada M, Namba H, Nii S (1997) High resolution immuno-scanning electron microscopy using a noncoating method: study of herpes simplex virus glyco­proteins on the surface of virus particles and infected cells. J Electron Microsc 46: 101–110Google Scholar
  18. 18.
    Santos RA, Padilla JA, Hatfield CC, Grose C (1998) Antigenic variation of varicella zoster virus Fc receptor gE: loss of a major B cell epitope in the ectodomain Virology 249: 21–31Google Scholar
  19. 19.
    Weigle KA, Grose C (1983) Common expression of varicella-zoster virus glycoproteins antigens in vitro and in chickenpox and zoster vesicles. J Infect Dis 148: 630–638PubMedCrossRefGoogle Scholar
  20. 20.
    Weigle KA, Grose C (1984) Molecular dissection of the humoral response to individual varicella-zoster viral proteins during chickenpox, quiescence, reinfection and reactiva­tion. J Infect Dis 149: 741–749PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2001

Authors and Affiliations

  • J. A. Padilla
    • 1
    • 2
  • C. Grose
    • 1
  1. 1.Departments of Microbiology & PediatricsUniversity of IowaIowa CityUSA
  2. 2.Department of PediatricsUniversity of Iowa HospitalIowa CityUSA

Personalised recommendations