Investigation of varicella-zoster virus variation by heteroduplex mobility assay
Heteroduplex mobility assays of 37 regions were performed on ten UK isolates of varicella zoster virus, four from cases of zoster, and six from cases of chickenpox. The variation between isolates was found to be 0.061%, which is at least five times lower than any other member of the human herpesvirus family. Fifteen of the 37 regions tested had 29 single nucleotide polymorphisms, and over half the polymorphisms were located in four gene fragments. Of the 29 SNPs, eleven were non-synonymous and these were clustered in six genes. Isolates from a child and her mother to whom she had transmitted the virus, were identical at every locus tested. All other viruses could be distinguished by a combination of SNPs and length polymorphisms of the repeat regions R1, R2 and R5.
KeywordsHerpes Simplex Virus Type Herpes Zoster Varicella Zoster Virus Heteroduplex Mobility Assay Heteroduplex Band
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- 3.Ganguly A, Rock MJ, Prockop DJ (1993) Conformation-sensitive gel electrophoresis for rapid detection of single-base differences in double stranded PCR products and DNA fragments: Evidence for solvent-induced bends in DNA heteroduplexes. Proc Natl Acad Sci USA 90: 10325–10329PubMedCrossRefGoogle Scholar
- 11.Poole LJ, Zong J-C, Ciufo DM, Alcendor DJ, Cannon JS, Ambinder R, Orenstein JM, Reitz MS, Haywards GS (1999) Comparison of genetic variability at multiple loci across the genomes of the major subtypes of Kaposi’s sarcoma-associated herpesvirus reveals evidence for recombination and for two distinct types of open reading frame K15 alleles at the right-hand end. J Virol 73: 6646–6660PubMedGoogle Scholar
- 12.Sakaoka H, Kurita K, Iida Y, Takada S, Umene K, Tae Kim Y, Shang Ren C, Nahmias AJ (1994) Quantitative analysis of genomic polymorphism of herpes simplex virus type 1 strains from six countries: studies of molecular evolution and molecular epidemiology of the virus. J Gen Virol 75: 513–527PubMedCrossRefGoogle Scholar