Abstract
Since the first work on culture of isolated plant cells and tissues in artificial nutrient solutions attempted by Gottlieb Haberlandt (1902) in Graz, Austria, 100 years ago, several practical applications have been derived by combining classical forest tree improvement programmes and tissue culture techniques, in particular for conifers and eucalyptus species, which are today the basis of a forest tree biotechnology industry, e.g. Silvagen and Arborgen. Many reviews are covering the topics of forest tree biotechnology and micropropagation (Ahuja 1988; 1993, Bonga and Durzan 1982, 1987a-c; Bajaj 1986, 1989, 1991 Jain et al. 1995a-c, 1999). In this context, new silvicultural concepts such as plantation or clonal forestry are widely discussed to cope with the expected increasing demand for wood during the next few decades (Fenning and Gershenzon 2002). Experiments with tissue cultures of woody species have been ongoing for decades. Research on tissue culture of forest tree species started very early in the 1930s, with callus and cell suspensions. The first successful callus proliferation and adventitious bud regeneration from cambial tissue was achieved by Gautheret in 1940 with English elm (Ulmus campestris). The first complete plants from tissue culture of a tree species were regenerated by Winton in 1968 from leaf explants of black cottonwood (Populus trichocarpa). Although there are some examples of successful regeneration of trees via protoplasts (reviewed by Tibok et al. 1995), the importance of this technology is negligible. Thorpe et al. (1991)counting the number of trees which can be micropropagated found about 70 angiosperm species and 30 gymnosperm species. This is a small fraction, compared to the total number of 1000 plant species which were accessible for micropropagation at that time. Today, the most efficient culture techniques for trees are somatic embryogenesis and organogenesis, i.e. axillary shoot regeneration. In addition to general tissue culture-related challenges (e.g. production of chimeras, somaclonal variation, endogenous bacterial contamination), regeneration of woody plant species is still considered recalcitrant because of effects related to ontogenetic ageing.
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References
Ahuja M. R., Krusche D., Melchior G. H. (1988) Determination of plantlet regeneration capacity of selected aspen clones in vitro. In: Ahuja M. R. (ed.) Somatic cell genetics of woody plants. Kluwer Academic Publishers, Dordrecht, pp. 127–135.
Ahuja M. R. (ed.) (1988) Somatic cell genetics of woody plants. Kluwer Academic Publishers, Dordrecht.
Ahuja M. R. (ed.) (1993) Micropropagation of woody plants. Kluwer Academic Publishers, Dordrecht.
Ake S., Grillet L., Labert C. (1991) Plane trees (Platanus spp.). In: Bajaj Y. P. S. (ed.) Biotechnology in agriculture and forestry, vol. 16, Trees III. Springer, Berlin Heidelberg, pp. 191–210.
Bajaj Y. P. S. (ed.) (1986, 1989, 1991) Biotechnology in agriculture and forestry, trees I, II, III Springer, Berlin Heidelberg.
Ballester A., Sanchez M. C., San-Jose M. C., Vieitez F. J., Vieitez A. M. (1990) Development of rejuvenation methods for in vitro establishment, multiplication and rooting of mature trees. In: Rodriguez R., Sanchez T. R., Durzan D. (eds.) Plant aging: basic and applied approaches. Plenum Press, New York, pp. 43–49.
Ballester A., Sanchez M. C., Vieitez A. M. (1996) Combination of ex vitro and in vitro manipulations for rejuvenation of hardwood species. In: Thomson D., Welander M. (eds.) Annual Report of CA AIR3–CT94–2202: Molecular and morphological markers for juvenility, maturity, rejuvenation and somatic embryogenesis in woody plant species — the biotechnological approach. EC DG XII-E, pp. AIR3–CT94.
Bayliss M. W. (1980) Chromosomal variation in plant tissue culture. Int. Rev. Cytol. 11A: 113–143.
Berros B., Albuerne M., Rodriguez R. (1994) Biochemical and morphological markers in hazelnut asexual embryogenesis. Proceedings of the IUFRO Working Party S2.04–07 Somatic Cell Genetics, Valsain, Spain, 04–07 Oct. 1993, 04–07.
Bonga J. M. (1987) Clonal propagation of mature trees: problems and possible solutions. In: Bonga J. M., Durzan D. J. (eds.) Cell and tissue culture in Forestry. Vol. 1. General principles and biotechnology. Martinus Nijhoff Publishers, Dordrecht, pp. 249–271.
Bonga J. M., Durzan D. J. (1982) Tissue culture in forestry, vol. 1. General principles and biotechnology. Martinus Nijhoff Publishers, Dordrecht.
Bonga J. M., Durzan D. J. (1987a) Cell and tissue culture in forestry, vol. 1. General principles and biotechnology. Martinus Nijhoff Publishers, Dordrecht.
Bonga J. M., Durzan D. J. (1987b) Cell and tissue culture in forestry, vol. 2. Specific principles and methods: growth and developments. Martinus Nijhoff Publishers, Dordrecht.
Bonga J. M., Durzan D. J. (1987c) Cell and tissue culture in forestry, vol. 3. Case histories: Gymnosperms, angiosperms and palms. Martinus Nijhoff Publishers, Dordrecht.
Bowles D. J. (1990) Defense related proteins in higher plants. Annual Reviews of Biochemistry 59: 873–907.
Chalupa V. (1987) European Hardwoods. In: Bonga J. M., Durzan, D. J. (eds.) Cell and tissue culture in forestry, vol. 3. Nijhoff Publishers, Dordrecht, pp. 224–246.
Chalupa A. V. (1990) Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.) and linden (Tilia cordata Mill.). Plant Cell Rep. 9: 398–401.
Chaturvedi H. C., Mitra G. C. (1975) A shift in morphogenetic pattern in Citrus callus tissue during prolonged culture. Ann. Bot. 39: 683–687.
Corredoira E., Vieitez A. M., Ballester A. (2002) Somatic embryogenesis in elm. Ann. Bot. 89: 637–644.
Cvikrova M., Mala J., Eder J., Hrubcova M., Vagner M. (1998) Abscisic acid, polyamines and phenolic acids in sessile oak somatic embryos in relation to their conversion potential. Plant Physiol. Biochem. 36: 247–255.
Cuenca B., San-Jose M. C., Martinez M. T., Ballester A., Vieitez A. M. (1999) Somatic embryogenesis from stem and leaf explants of Quercus robur L. Plant Cell Rep 18: 538–543.
DeVerno L. L. (1995) An evaluation of somaclonal variation during somatic embryogenesis. In: Jain S., Gupta P., Newton R. (eds.) Somatic embryogenesis in woody plants. vol. 1: 361–377.
Druart P. (1980) Plant regeneration from root callus of different Prunus species. Scientia Hort. 12: 339–342.
Diner A. M., Karnosky D. F. (1987) Tissue culture application to forest pathology and pest control. In: Bonga J. M., Durzan D. J. (eds.) Cell and tissue culture in forestry, vol. 2. Martinus Mijhoff Publishers, Dordrecht, pp. 351–373.
Endemann M., Wilhelm E. (1999) Factors influencing the induction and viability of somatic embryos of Quercus robur L. Biologia Plantarum 42: 499–504.
Endemann M., Hristoforoglu K., Stauber T., Wilhelm E. (2001) Assessment of age-related polyploidy in Quercus robur L. somatic embryos and regenerated plants using DNA flow cytometry. Biologia Plantarum 44(3): 339–343.
Einspahr D. W., Wann S. R. (1985) Use if tissue culture techniques in a hardwood tree improvement program. In: Schmidtling R. C., Griggs M. (eds.) Proc. 18th South. For. Tree Improv. Conf. May 21–23, 1985, Long Beach, MS, 21–23.
Evers P., Donkers J., Prat A., Vermeer E. (1988) Micropropagation of forest trees through tissue culture. Pudoc Wageningen, The Netherlands.
Evers P., Haanstra L., Vermeer E., Van Eeeden S. (1996) Influence of reversed phase change on micropropagation of Quercus robur. Plant Tissue Culture and Biotechn. 2(3): 148–154.
Fenning T. M., Gershenzon J. (2002) Where will the wood come from? Plantation forests and the role of biotechnology. Trends in Biotechnology (in press).
Filanova L. H., Bozhkov P. V., Brukhin V. B., Daniel G., Zhivotovsky B., Von Arnold S. (2000) Two waves of programmed cell death occur during formation and development of somatic embryos in the gymnosperm, Norway spruce. J. Cell Science 113: 4399–4411.
Gautheret R. J. (1940) Nouvelles recherches sur le bourgeonnement du tissu cambial d’ Ulmus campestris cultive in vitro. C.R. Acad. Sci. Paris 210: 744–746.
Giannino D., Ticconi C., Frugis G., Mele G., Florio S., Santini L., Cozza R., Bitonti M. B., Innocenti A. M., Mariotti D. (1999) Characterisation of juvenile and adult stages in woody species by means of ddRT-PCR markers and genes from heterologous species. Congress on “Applications of Biotechnology to Forest Genetics” in Vitoria-Gasteiz, Spain, 22–25 September 1999. Book of Abstracts.
Gingas V. M., Lineberger R. D. (1989) Asexual embryogenesis and plant regeneration in Quercus. Plant Cell, Tissue and Organ Culture 1: 191–203.
Greenwood M. S. (1987) Rejuvenation of forest trees. Plant Growth Reg. 6: 1–12.
Gresshoff P. M., Doy C. H. (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107: 161–170.
Haberlandt G. (1902) Culturversuche mit isolierten Pflanzenzellen. Sitzungsber. Akad. Wiss. Wien. Math. Nat. Cl. 111, Abt.1: 69–91.
Hackett R W., Murray J. R. (1996) Maturation or phase change. In: Thompson D., Welander M. (eds.) Annual Report of CA AIR3–CT94–2202: Molecular and morphological markers for juvenility, maturity, rejuvenation and somatic embryogenesis in woody plant species — the biotechnological approach EC DG XII-E, pp. AIR3–CT94.
Hebard F. V., Kaufman R B. (1978) Chestnut callus cultures: Tannin content and colonization by Endothia parasitica. Proc. Am. Chestnut Symp. Morgantown. WV West Virginia. Univ. Vooks, pp. 63–79.
Jain S., Gupta P., Newton R. (eds.) (1995a) Somatic embryogenesis in woody plants, vol. 1: History, molecular and biochemical aspects and applications. Kluwer Adademic Publishers, Dordrecht.
Jain S., Gupta R, Newton R. (eds.) (1995b) Somatic embryogenesis in woody plants, vol. 2: Angiosperms. Kluwer Adademic Publishers, Dordrecht.
Jain S., Gupta P., Newton R. (eds.) (1995c) Somatic embryogenesis in woody plants, vol. 3: Gymnosperms. Kluwer Adademic Publishers, Dordrecht.
Jain S., Gupta P., Newton R. (eds.) (1999) Somatic embryogenesis in woody plants vol. 4: Gymnosperms. Kluwer Adademic Publishers, Dordrecht.
Jones O. P. (1993) Propagation of apple in vitro. In: Ahuja M. R. (ed.) Micropropagation of woody plants. Kluwer Academic Publishers, Netherlands, pp. 169–186.
Kendurkar S. V., Nadgauda R. S., Phadke C. H., Jana M. M., Shirke S. V., Mascarenhas A. F. (1995) Somatic Embryogenesis in some woody angiosperms. vol. 1. In: Jain S. M., Gupta P. K., Newton R. J. (eds.) Somatic embryogenesis in woody plants. Kluwer Academic Publishers, Dordrecht, pp. 49–80.
Kim Y. W., Youn Y., Noh E. R., Kim J. C. (1997) Somatic embryogenesis and plant regeneration from immature embryos of five families of Quercus acutissima. Plant Cell Rep. 16: 869–873.
Jörgensen J. (1989) Embryogenesis in Quercus petraea and Fagus sylvatica. J. Plant Physiol. 132: 638–640.
Larkin P. J., Scowcroft W. R. (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214.
Lloyd G., McCown B. (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Proc. Int. Plant Propagators Soc. 30, pp. 421–427.
Malá J., Cvikrova H., Brezinov A., Hrubcová M., Eder J., Vagner M., Cvikrova M. (1999) Biochemical characteristics of oak somatic embryos. Book of Abstracts. Congress on “Applications of Biotechnology to Forest Genetics” in VitoriaGasteiz, Spain, 22–25 September 1999.
McComb J. A., Hinch J. M., Clarke A. E. (1987) Expression of field resistance in callus tissue inoculated with Phytophthora cinnamomi. Phytopathology 77: 347–351.
Merkle S. A. (1995) Strategies for dealing with limitations of somatic embryogenesis in hardwood trees. Plant Tissue Cult. Biotechn. 1(3): 112–121.
Merkle S. A., Parrot W. A., Flinn B. S. (1995) Morphogenic aspects of somatic embryogenesis. In: Thorpe T. A. (ed.) In vitro embryogenesis in plants. Kluwer Adademic Publishers, Dordrecht, pp. 155–203.
Morel G. (1944) Le développement du mildiou sur des tissus de vigne cultures in vitro. Compt. Rend. Hebdom. des Séances de l’Academie des Sciences, Paris 218: 50–52.
Morel G. (1948) XXX Recherches sur la culture associée de parasites obligatoires et de tissus végétaux Annales des éphiphyties (série Pathologie Végétale) 14:1–112.
Morselli M. F. (1989) Maple (Acer spp.) In: Bajaj Y. P. S. (ed.) Biotechnology in agriculture and forestry 5, Trees II. Springer, Berlin Heidelberg, pp. 246–274.
Murray J. R., Smith A. G., Hackett W. P. (1994) Differential dihydroflavonol reductase transcription and anthocyanin pigmentation in the juvenile and mature phases of ivy (Hedera helix L.). Planta 194: 102–109.
Novak J. (1998) Benefits of in vitro “Biotization” of plant tissue cultures with microbial inoculants. Review. In vitro Cell. Dev. Biol. Plant 34: 122–130.
Ostry M. E., Skilling D. D. (1992) Applications of tissue culture for studying tree defense mechanisms. In: Blanchette R. A., Biggs A. R. (eds.) Defense mechanisms of woody plants against fungi. Springer, Berlin, pp. 405–423.
Park Y. S., Barret, J. D., Bonga J. M. (1998) Application of somatic embryogenesis in high-value clonal forestry: deployment, genetic control and stability of cryopreserved clones. In vitro Cell. Dev. Biol. Plant 34: 231–239.
Pijut P. M., Domir S. C., Lineberger R. D., Schreiber L. R. (1990) Use of culture filtrates of Ceratocystis ulmi as a bioassay to screen for disease tolerant Ulmus americana. Plant Sci. 70: 191–196.
Rahmann M. H., Rajora O. P. (2001) Microsatellite DNA somaclonal variation in micropropagated trembling aspen (Populus tremuloides). Plant Cell. Rep. 20: 531–536.
Rani V., Raina S. N. (2000) Genetic fidelity of organized meristem-derived micropropagated plants: A critical reappraisal. In vitro Cell. Dev. Biol. Plant 36: 319–330.
Richards E. J. (1997) DNA methylation and plant development. Trends in Genetics 13: 319–323
Ruaud J. N., Paques M. (1995) Somatic embryogenesis and rejuvenation of trees. In: Jain S., Gupta P., Newton R. (eds.) Somatic embryogenesis in woody plants. Kluwer Academic Publisher, Dordrecht, vol. 1: 99–118.
Sanchez M. C., Ballester A., Vieitez A. M. (1997) Reinvigoration treatments for the micropropagation of mature chestnut trees. Ann. Sci. For. 54: 359–370.
Sauer U., Wilhelm E. (2002) Somatic embryogenesis from ovaries, developing ovules and immature zygotic embryos of European chestnut Castanea sativa Mill. (in prep).
Schafleitner R., Wilhelm E. (1997) Effect of virulent and hypovirulent Cryphonectria parasitica (Murr.) barr on the intercellular pathogen related proteins and on total protein pattern of chestnut (Castanea sativa mill.). Phys. Mol. Plant Pathol. 51: 323–332.
Schafleitner R., Wilhelm E. (1999a) Chestnut blight: Monitoring the host response with heterologous cDNA probes. Acta Hort. 494: 481–486.
Schafleitner R., Buchala A., Wilhelm E. (1999b) Class III chitinase expression and salicylic acid accumulation in chestnut (Castanea sativa L) after challenge with hypovirulent and virulent Cryphonectria parasitica (Muff.) barr. Phyton. 39: 191–196.
Schafleitner R., Wilhelm E. (2002a) Isolation of wound inducible genes from Castanea sativa stems and expression analysis in the bark tissue. Plant Physiol. Biochem. 40(3): 235–245.
Schafleitner R., Wilhelm E. (2002b) Assessment of stress gene expression in chestnut (Castanea sativa mill.) upon pathogen infection (C. parasitica (Murr.) barr.) and wounding. Forest Snow and Landscape Research (in press).
Schafleitner R., Wilhelm E. (2002c) Isolation of wound-responsive genes from chestnut (Castanea sativa Mill.) microstems by mRNA display and their differential expression upon wounding and infection with the chestnut blight fungus (Chryphonectria parasitica (Murr.) Barr.) (submitted).
Sotak R. J., Sommer H. E., Merkle S. A. (1991) Relation of the developmental stage of zygotic embryos of yellow-poplar to their somatic embryogenic potential. Plant Cell Reports 10(4): 175–178.
Stermer B. A., Scheffer R. P., Hart J. H. (1984) Isolation of toxins of Hypoxylon mammatum and demonstration of some toxic effects on selected clones of Populus tremuloides. Phytopathology 74: 654–658.
Sunderlikova V., Wilhelm E. (2002) High accumulation of legumin and Lea mRNAs during maturation are associated with increased conversion frequency of somatic embryos from pedunculate oak (Quercus robur L.) Protoplasma (in press).
Thorpe T. A., Harry I. S., Kumar P. P. (1991) Application of micropropagation to forestry. In: Debergh P. C., Zimmerman P H (eds.) Micropropgation: technology and application. Kluwer Academic Publishers, Dordrecht, pp. 311–336.
Tibok A., Power J. B., Davey M. R. (1995) Progress in protoplast technology for woody angiosperms. In: Jain S., Gupta P., Newton R. (eds.) Somatic embryogenesis in woody plants. vol. 1. Kluwer Adademic Publishers, Dordrecht, pp. 143–166.
Toribio M., Celestino C., Gallego J., Martinez I. (1998) Induction of somatic embryogenesis in tissues from mature oak trees. COST 822, WG 1 “Physiology and Control of Plant Propagation in vitro”. Meeting in Lisse, The Netherlands.
Valentine F., Baker S., Belanger R., Manion P., Griffin D. (1988) Screening for resistance to Hypoxylon mammatum in Populus tremuloides callus and micropropagted plantlets. In: Ahuja M. A. (ed.) Somatic cell genetics of woody plants. Kluwer Academic Publishers, Dordrecht, p. 181.
Van der Linden C. G. W., Rus-Kortekaas M. J. M., Smulders R. (1999) MADS-box genes as putative markers for juvenile and mature phase in woody species. Congress on “Applications of Biotechnology to Forest Genetics” in VitoriaGasteiz, Spain, 22–25 September 1999, Book of Abstracts.
Vardi A., Epstein E., Breiman A. (1986) Is the Phytophthtora citrophthora culture filtrate a reliable tool for the in vitro selection of resistant Citrus variants? Theor. Appl. Genet. 72: 569–574.
Vieitez A. M., Ballester A., Vieitez M. L., Vieitez E. (1983) In vitro plantlet regeneration of mature chestnut. J. Hort. Sci. 58: 457–463.
Welander M. (1993) Micropropagation of birch. In: Ahuja M. A. (ed.) Micropropagation of woody plants. Kluwer Adademic Publishers, Dordrecht.
Wilhelm E., Arthofer W., Schafleitner R., Krebs B. (1998) Bacillus subtilis an endophyte of chestnut (Castanea sativa) as antagonist against chestnut blight (Cryphonectria parasitica). Plant Cell, Tissue and Organ Culture 52: 105–108.
Wilhelm E. (1999) Micropropagation of juvenile Acer pseudoplatanus via adventitious shoot formation by use of thidiazuron. Plant Cell, Tissue and Organ Culture 57: 57–60.
Wilhelm E. (2000) Somatic embryogenesis in oak (Quercus spp.). In vitro Cell. Dev. Biol. Plant 36: 349–357.
Winton L. W. (1968) The rooting of liquid grown aspen cultures. Am. J. Bot. 55: 159–167.
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Wilhelm, E. (2003). Tissue culture of broad-leafed forest tree species. In: Laimer, M., Rücker, W. (eds) Plant Tissue Culture. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6040-4_12
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