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Recombinant immunoblot assay for hepatitis C virus antibody in chronic hepatitis

  • G. Taliani
  • M. C. Badolato
  • R. Lecce
  • C. De Bac
  • E. De Marzio
  • C. Balsano
  • M. Artini
  • M. Levrero
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Abstract

Testing for hepatitis C virus by ELISA requires confirmation by recombinant immunoblot assay (RIBA). The first-generation RIBA uses the same antigen as used in the ELISA and one further antigen. A second-generation RIBA in which two further antigens are present, detects positivity that is not found by either the ELISA or the original RIBA. Consequently, although it is adequate to test Elisa positive sera with the first-generation RIBA, the second-generation assay is recommended for confirming negativity.

Keywords

Chronic Liver Disease Chronic Patient Virus Antibody Positive Seron HBsAg Positive Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Skidmore S. Recombinant immunoblot assay for hepatitis C antibody (1990) Lancet 335: 1346Google Scholar
  2. 2.
    Van Der Poel CL, Cuypers HTM, Reesink HW et al (1991) Confirmation of hepatitis C virus infection by new four antigen recombinant immunoblot assay. Lancet 337: 317–319PubMedCrossRefGoogle Scholar
  3. 3.
    Wong DC, Diwan AR, Rosen L et al (1990) Non-specificity of anti-HCV test for seroepidemiological analysis. Lancet i: 750–751Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. Taliani
    • 1
  • M. C. Badolato
    • 1
  • R. Lecce
    • 1
  • C. De Bac
    • 1
  • E. De Marzio
    • 1
  • C. Balsano
    • 1
  • M. Artini
    • 1
  • M. Levrero
    • 1
  1. 1.Institute of Tropical Diseases“La Sapienza” University and Fondazione A. Cesalpino, I Clinica Medica, “La Sapienza” UniversityRomeItaly

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