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Detection and characterization of hepatitis C virus sequence in the serum of a patient with chronic HCV infection

  • E. Schreier
  • K. Fuchs
  • M. Höhne
  • M. Motz
  • R. Zachoval
  • J. Esteban
  • S. Dittmann
  • F. Deinhardt
  • M. Roggendorf
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

A cDNA fragment corresponding to the nonstructural gene region of Hepatitis C virus was cloned and sequenced. cDNA was obtained by reverse transcription of viral RNA extracted from serum of a German patient with chronic post transfusion hepatitis. “Nested” PCR resulted in a cDNA fragment of 345 nt. The sequence showed a homology of 96% to the American prototype HCV.

Keywords

Polymerase Chain Reaction Product cDNA Fragment German Patient Japanese Isolate Expect Polymerase Chain Reaction Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • E. Schreier
    • 1
  • K. Fuchs
    • 2
  • M. Höhne
    • 1
  • M. Motz
    • 3
  • R. Zachoval
    • 4
  • J. Esteban
    • 5
  • S. Dittmann
    • 1
  • F. Deinhardt
    • 2
  • M. Roggendorf
    • 2
    • 6
  1. 1.Robert Koch-Institute of the Federal Health OfficeBerlinGermany
  2. 2.Max-von-Pettenkofer-InstituteUniversity of MunichGermany
  3. 3.MikrogenMunichGermany
  4. 4.Klinikum GroßhadernUniversity of MunichFederal Republic of Germany
  5. 5.Ciudad SantariaVail d’HebronBarcelonaSpain
  6. 6.Institute of Medical Virology and ImmunologyUniversity ClinicsEssenFederal Republic of Germany

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