Detection and characterization of hepatitis C virus sequence in the serum of a patient with chronic HCV infection
A cDNA fragment corresponding to the nonstructural gene region of Hepatitis C virus was cloned and sequenced. cDNA was obtained by reverse transcription of viral RNA extracted from serum of a German patient with chronic post transfusion hepatitis. “Nested” PCR resulted in a cDNA fragment of 345 nt. The sequence showed a homology of 96% to the American prototype HCV.
KeywordsPolymerase Chain Reaction Product cDNA Fragment German Patient Japanese Isolate Expect Polymerase Chain Reaction Product
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