Hepatitis C viral RNA in serum of patients with chronic non-A, non-B hepatitis: detection by the polymerase chain reaction using multiple primer sets

  • Karen Cristiano
  • A. M. Di Bisceglie
  • J. H. Hoofnagle
  • S. M. Feinstone
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)


The recently introduced antibody test for hepatitis C virus (HCV) infection has proven to have certain limitations. Since HCV itself is usually present in clinical specimens at very low titers, a useful assay for the virus must have very high sensitivity. We have developed a simple, highly sensitive assay for HCV RNA based on the polymerase chain reaction (PCR). In this test, RNA extracted from HCV infected serum or plasma is used as the template for double PCR with nested primers. Sensitivity studies demon¬strate that this assay is able to detect HCV at or beyond the sensitivity level of chimpanzee infectivity. We tested, with several sets of nested primers, 40 patients with chronic non-A, non-B hepatitis (36 seropositive and 4 seroneg¬ative) and found that 35/40 were PCR positive including all 4 seronegative patients. Normal human plasma and plasma from hepatitis B infected patients did not react in this test. This assay has proven to be valuable for determining the presence of HCV in various samples; furthermore, it offers the possibility of diagnosis of HCV infection in seronegative patients.


Nest Primer Seronegative Patient Chimpanzee Infectivity Polymerase Chain Reaction Titer Result Polymerase Chain Reaction Amplification 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Karen Cristiano
    • 1
    • 3
  • A. M. Di Bisceglie
    • 2
  • J. H. Hoofnagle
    • 2
  • S. M. Feinstone
    • 1
  1. 1.Laboratory of Hepatitis Research, Division of VirologyCenter for Biologies Evaluation and Research, Food and Drug AdministrationBethesdaUSA
  2. 2.Liver Diseases Section, National Institutes of Diabets and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaUSA
  3. 3.Laboratorio di Virologia, Reparto Virus EpatiticiInstituto Superiore di SanitàRomaItaly

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