Properties of a recombinant yeast-derived hepatitis B surface antigen containing S, preS2 and preSl antigenic domains
Yeast cells have been engineered to express mixed HBsAg particles containing the S and a modified large (L*) protein. Their construc¬tion resulted in reduced protease sensitivity, reduced glycosylation and complete inactivation of the polymerized human albumin binding site. The particles exposed the S, preSl and preS2 antigenic determinants and induced an immune response against the three domains. Highly purified preparations have been obtained and are presently being tested in human volunteers.
KeywordsAntigenic Domain Chronic Hepatitis Type HBsAg Particle preS Domain preS2 Response
Unable to display preview. Download preview PDF.
- 2.André FE, Safary A (1988) Clinical experience with a yeast-derived hepatitis B vaccine. In: Zuckerman AJ (ed) Viral hepatitis and liver disease. Liss, New York, pp 1025–1030Google Scholar
- 5.Gerety RJ (1988) Recombinant hepatitis B vaccines. In: Zuckerman AJ (ed) Viral hepatitis and liver disease. Liss, New York, pp 1017–1024Google Scholar
- 7.Heermann KH, Goldmann V, Schwartz W, Seyffarth T, Baumgarten H, Gerlich WHGoogle Scholar
- (1984).Large surface proteins of hepatitis B virus containing the preS sequence. J Virol 52: 396–402Google Scholar
- 9.Iwarson S, Tabor E, Thomas HC, Goodall A, Waters J, Snoy P, Shih JWK, Gerety RJGoogle Scholar
- (1985).Neutralization of hepatitis B virus infectivity by a murine monoclonal antibody. An experimental study in chimpanzee. J Med Virol 16: 89–96Google Scholar
- 11.Milich DR, Mc Namara MK, Mc Lachlan A, Thornton GB, Chisari FV (1985) Distinct H-2 linked regulation of T-cell responses to pre-S and S regions of the same hepatitis B surface antigen polypeptide allows circumvention of non responsiveness to the S region. Proc Natl Acad Sci USA 82: 8168–8172PubMedCrossRefGoogle Scholar