Deletion and insertion mutants of HBsAg particles
We have found previously that hybrid 22-nm HBsAg particles can be created by insertion of short antigenic sequences into the HBV major envelope protein . We have now performed a detailed deletion mutagenesis of the S gene of HBV encoding HBsAg. Deletion of the 51 C-terminal amino acids including most of the third and all of the fourth hydrophobic domain of the S protein did not affect particle assembly and secretion. However, secretion of 22-nm particles was abolished by minor deletions in the N-terminal region. Insertion and deletion/substitution mutants carrying a poliovirus epitope at the N-terminus and the preSl region at the C-terminus have been characterized.
KeywordsHepG2 Cell Carboxy Terminus Hydrophilic Domain Cellular Supernatant HBsAg Particle
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- Authors’ address: Dr. U. Machein, Institute for Medical Microbiology, University of Mainz, D-W-6500-Mainz, Federal Republic of Germany.Google Scholar