Advertisement

Deletion and insertion mutants of HBsAg particles

  • U. Machein
  • R. Nagel
  • R. Prange
  • A. Clemen
  • R. E. Streeck
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

We have found previously that hybrid 22-nm HBsAg particles can be created by insertion of short antigenic sequences into the HBV major envelope protein [1]. We have now performed a detailed deletion mutagenesis of the S gene of HBV encoding HBsAg. Deletion of the 51 C-terminal amino acids including most of the third and all of the fourth hydrophobic domain of the S protein did not affect particle assembly and secretion. However, secretion of 22-nm particles was abolished by minor deletions in the N-terminal region. Insertion and deletion/substitution mutants carrying a poliovirus epitope at the N-terminus and the preSl region at the C-terminus have been characterized.

Keywords

HepG2 Cell Carboxy Terminus Hydrophilic Domain Cellular Supernatant HBsAg Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Delpeyroux F, Chenciner N, Lim A, Malpièce Y, Blondel B, Crainic R, van der Werf S, Streeck RE (1986) A poliovirus neutralization epitope expressed on hybrid hepatitis B surface antigen particles. Science 233: 472–475PubMedCrossRefGoogle Scholar
  2. 2.
    Delpeyroux F, Chenciner N, Lambert M, Malpièce Y, Streeck RE (1987) Insertions in the hepatitis B surface antigen: Effect on assembly and secretion of 22-nm particles from mammalian cells. Mol Biol 195: 343–350CrossRefGoogle Scholar
  3. 3.
    Eble BE, MacRae DR, Lingappa VR, Ganem D (1987) Multiple topogenic sequences determine the transmembrane orientation of hepatitis B surface antigen. Mol Cell Biol 7: 3591–3601PubMedGoogle Scholar
  4. Authors’ address: Dr. U. Machein, Institute for Medical Microbiology, University of Mainz, D-W-6500-Mainz, Federal Republic of Germany.Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • U. Machein
    • 1
  • R. Nagel
    • 1
  • R. Prange
    • 1
  • A. Clemen
    • 1
  • R. E. Streeck
    • 1
  1. 1.Institute for Medical MicrobiologyUniversity of MainzMainzFederal Republic of Germany

Personalised recommendations