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HBc and HBe specificity of monoclonal antibodies against complete and truncated HBc proteins from E. coli

  • E. Korec
  • W. H. Gerlich
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

We have prepared and used monoclonal antibodies against various populations of full-length and truncated hepatitis B core proteins in order to distinguish between epitopes of HBcAg and HBeAg. Our results show that various epitopes are specific for the different proteins. Certain epitopes, however, are ubiquitous to HBc/e proteins and these are probably exposed on the surface of HBc particles.

Keywords

Core Antigen Associate Protein Kinase Virus Associate Protein Carboxy Terminal Domain 41F4 IgG2A 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Korec E, Korcova J, König J, Hlozanek I (1989) Detection of antibodies against hepatitis B core antigen using the avidin-biotin system. J Virol Method 24: 312–326CrossRefGoogle Scholar
  2. 2.
    Melegari M, Bruss V, Gerlich WH (1991) The arginine-rich carboxyterminal domain is necessary for RNA packaging by hepatitis B core protein. In: Hollinger FB, Lemon SM, Margolis H (eds) Viral hepatitis and liver disease. Williams & Wilkins, Baltimore, pp 164–168Google Scholar
  3. 3.
    Korec E, Dostalova V, Korcova J, Mancal P, König J, Borisova G, Cibinogen V, Pumpen P, Gren E, Hlozanek I (1990) Monoclonal antibodies against hepatitis B e antigen: production, characterization, and use for diagnosis. J Virol Meth 28: 165–170CrossRefGoogle Scholar
  4. 4.
    Gerlich WH, Goldmann U, Müller R, Stibbe W, Wolff W (1982) Specificity and localization of the hepatitis B virus associated protein kinase. J Virol 42: 761–766PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • E. Korec
    • 1
  • W. H. Gerlich
    • 2
  1. 1.Institute of Molecular GeneticsPragueCSFR
  2. 2.Department of Medical MicrobiologyUniversity of GöttingenFederal Republic of Germany

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