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Detection of HBV DNA by PCR in serum from an HBsAg negative blood donor implicated in cases of post-transfusion hepatitis B

  • Helene Norder
  • B. Hammas
  • J. Larsen
  • K. Skaug
  • L. O. Magnius
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

An HBsAg negative blood donor, and three of her recipients, who developed HBsAg positive post-transfusion hepatitis B, were all positive for serum HBV DNA by polymerase chain reaction (PCR), and by subtype discriminating PCR were found to harbour HBV specifying ayw. Thus HBV DNA may be detected and sub-typed by PCR in infectious HBsAg negative individuals.

Keywords

Specific Oligonucleotide Prime Oligonucleotide Prime Pair Bacteriological Laboratory Detectable HBsAg Simplify Polymerase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Larsen J, Hetland G, Skaug K (1990) Post-transfusion hepatitis B transmitted by blood from a surface-antigen negative hepatitis B carrier. Transfusion 30: 431–432PubMedCrossRefGoogle Scholar
  2. 2.
    Norder H, Hammas B, Magnius LO (1990) Typing of hepatitis B virus genomes by a simplified polymerase chain reaction. J Med Virol 31: 215–221PubMedCrossRefGoogle Scholar
  3. 3.
    Okamoto H, Imai M, Tsuda F, Sakugawa H, Sastrosoewignjo R, Miyakawa Y, Mayumi M (1988) Typing hepatitis B virus by homology in nucleotide sequence: Comparison of surface antigen subtypes. J Gen Virol 69: 2575–2583PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Helene Norder
    • 1
  • B. Hammas
    • 1
  • J. Larsen
    • 2
  • K. Skaug
    • 3
  • L. O. Magnius
    • 1
  1. 1.Department of VirologyThe National Bacteriological LaboratoryStockholmSweden
  2. 2.Blood Bank and Department of ImmunologyUllevaal HospitalNorway
  3. 3.Microbiological LaboratoryUllevaal HospitalOsloNorway

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