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PCR analysis of HBV infected sera: relationship between expression of pre-S antigens and viral replication

  • A. R. Garbuglia
  • A. Manzin
  • A. Budkowska
  • G. Taliani
  • C. Delfini
  • G. Carloni
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

In order to determine the biological significance of the pre-S antigens in HBV infection, HBsAg sera were tested for the presence of pre-S 1 and pre-S2. HBV DNA was detected by spot-hybridization and PCR. The data show a complete correlation between pre-S antigenemia and HBV DNA replication in anti-HBe positive cases. PCR but not spot-hybridization was adequately sensitive to also detect HBV DNA in roughly half of the preS negative sera as well. Thus PCR appears to be a valuable technique for detection of potentially infectious anti-HBe carriers.

Keywords

Southern Blot Hybridization Negative Seron Complete Correlation Enzyme Immunoassay Method Enzyme Immune Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Neurath AR, Kent SBH (1988) Adv Virus Res 34: 65–142PubMedCrossRefGoogle Scholar
  2. 2.
    Budkowska A et al (1986) Hepatology 6: 360–368PubMedCrossRefGoogle Scholar
  3. 3.
    Carloni G et al (1987) J Med Virol 21: 15–23PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. R. Garbuglia
    • 1
  • A. Manzin
    • 2
  • A. Budkowska
    • 3
  • G. Taliani
    • 4
  • C. Delfini
    • 1
  • G. Carloni
    • 5
  1. 1.Cell BiologyIstituto Superiore di SanitàRomeItaly
  2. 2.Institute of MicrobiologyUniversity of AnconaItaly
  3. 3.Institut PasteurParisFrance
  4. 4.Institute of Infectious DiseasesUniversity of RomeItaly
  5. 5.Institute of Experimental MedicineC.N.R.RomeItaly

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