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Truncated pre-S/S proteins transactivate multiple target sequences

  • G. Natoli
  • C. Balsano
  • M. L. Avantaggiati
  • E. De Marzio
  • M. Artini
  • D. Collepardo
  • E. Elfassi
  • M. Levrero
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

In order to investigate the transactivational function of HBV truncated preS/S proteins we have constructed two sets of plasmids and have tested their transactivational potential on the c-myc regulatory sequences and the TPA-responsive element. We found that preS/S proteins only become transactivationally active when truncated at the carboxy terminal end. Furthermore, using immunofluorescence microscopy we determined that the proteins are located exclusively in the cytoplasm, apparently ruling out DNA binding and activation of factors in the nucleus.

Keywords

Human Immunodeficiency Virus Type Transactivational Potential Surface Protein Gene Major Late Promoter Adenoviral Major Late Promoter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. Natoli
    • 1
  • C. Balsano
    • 1
  • M. L. Avantaggiati
    • 1
  • E. De Marzio
    • 1
  • M. Artini
    • 1
  • D. Collepardo
    • 1
  • E. Elfassi
    • 2
  • M. Levrero
    • 1
  1. 1.Laboratory of Genetic ExpressionFondazione A. Cesalpino, I Clinica MedicaRomeItaly
  2. 2.Unitè d’Immunologie MicrobienneInstitute PasteurParisFrance

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