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Hepatitis B virus specific transcripts in peripheral blood mononuclear cells

  • Margherita Melegari
  • P. P. Scaglioni
  • C. Pasquinelli
  • F. Manenti
  • Erica Villa
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 4)

Summary

We report on the analysis of HBV transcription in peripheral blood mononuclear cells of chronically infected patients by polymerase chain reaction amplification. Our results suggest that in these cells gene expression occurs either as pregenomic or subgenomic transcripts.

Keywords

Peripheral Blood Mononuclear Cell Strand Primer Viremic Patient Guanidine Isothiocyanate Method Total Peripheral Blood Mononuclear Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Davison F, Alexander GJM, Anastssakos C, Fagan EA, Williams R (1987) Leucocyte hepatitis B virus DNA in acute and chronic hepatitis B virus infection. J Med Virol 22: 379–385PubMedCrossRefGoogle Scholar
  2. 2.
    Davison F, Alexander GJM, Trowbridge R, Fagan EA, Williams R (1987) Detection of hepatitis B virus DNA in spermatozoa, urine, saliva and leucocytes of chronic HBsAg carriers. A lack of relationship with serum markers of replication. J Hepatol 14: 37–44Google Scholar
  3. 3.
    Hadchouel M, Pasquinelli C, Fournier JC, Hugon RN, Scotto J, Bernard O, Brechot C (1988) Detection of mononuclear cells expressing hepatitis B virus in peripheral blood from HBsAg positive and negative patients by in situ hybridization. J Med Virol 24: 27–32PubMedCrossRefGoogle Scholar
  4. 4.
    Korba BE, Wells F, Tennant BC, Cote PJ, Gerin JL (1987) Lymphoid cells in the spleens of woodchuck hepatitis virus infected woodchucks are a site of active viral replication. J Virol 61: 1318–1324PubMedGoogle Scholar
  5. 5.
    Lie-Injo LE, Balasegaram M, Lopez CG, Herrera AR (1983) Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma. DNA 4: 301–308CrossRefGoogle Scholar
  6. 6.
    Pasquinelli C, Lauré F, Chatenoud L, Beaurin G, Gazengel C, Bismuth H, Degos F, Tiollais P, Bach JF, Brechot C (1986) Hepatitis B virus DNA in mononuclear blood cells. A frequent event in hepatitis B surface antigen positive and negative patients with acute and chronic liver disease. J Hepatol 3: 95–103Google Scholar
  7. 7.
    Pasquinelli C, Melegari M, Villa E, Scaglioni PP, Seidenari M, Mongiardo N, De Rienzo B, Manenti F (1990) Hepatitis B Virus infection of peripheral blood mononuclear cells is common in acute and chronic hepatitis. J Med Virol 31: 135–140PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Margherita Melegari
    • 1
  • P. P. Scaglioni
    • 1
  • C. Pasquinelli
    • 2
  • F. Manenti
    • 1
  • Erica Villa
    • 1
  1. 1.Chair of GastroenterologyUniversity of ModenaModenaItaly
  2. 2.Department of Molecular and Experimental MedicineResearch Institute of Scripps ClinicLa JollaUSA

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