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Sodium-dependent Metabolism and Transport of γ-Aminobutyric Acid in Subcellular Particles from Brain

  • S. Varon
  • H. Weinstein
  • E. Roberts
Conference paper

Abstract

The properties of γ-aminobutyric acid (γABA) are of great interest in the biochemistry and physiology of the vertebrate central nervous system [1]. γABA is found to associate with mitochondria, microsomes and nerve ending particles, all of which can be sedimented from (mouse brain homogenates in 0.25 M sucrose by centrifugation between 1,500 and 15,000 X g. When a pellet is resuspended in 0.2 M NaCl (buffered at pH 7.3) and incubated at 0–4° C in the presence of 2–14C-γABA, a rapid accumulation of radioactivity in the particles occurs. This accumulation is dependent on the presence of Na+ ions. A series of studies, reviewed in the preceding paper [2], has led to the postulation of an equilibrating carrier mechanism for the uptake of γABA at 0°C. The Na-dependence of this mechanism lies in a Na+ ion requirement for the carrier to bind γABA.

Keywords

Preceding Paper Subcellular Particle Vertebrate Central Nervous System Experiment filS Mouse Brain Homogenate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    Roberts, E. (ed.), 1960: “Inhibition of the Nervous System and γ-Aminobutyric Acid.” Pergamon Press, New York.Google Scholar
  2. [2]
    Weinstein, H., S. Varon, and E. Roberts, 1965: These Proceedings, p. 318.Google Scholar
  3. [3]
    Varon, S., H. Weinstein, C. F. Baxter, and É. Roberts, 1965: Riochem. Pharmacol. 14, 1755.CrossRefGoogle Scholar
  4. [4]
    Varon, S., H. Weinstein, and E. Robert: (in preparation).Google Scholar
  5. [5]
    Salganicoff, L., and E. DeRobertis, 1963: Life Sciences 2, 85.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1967

Authors and Affiliations

  • S. Varon
    • 1
  • H. Weinstein
    • 1
  • E. Roberts
    • 1
  1. 1.Department of BiochemistryCity of Hope Medical CenterDuarteUSA

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