X-ray Diffraction Studies of Cell Membranes

  • J. B. Finean
Conference paper


For X-ray diffraction studies one requires a repeating pattern of structure that is both regular and extensive and the diffracting system must constitute a large proportion of the specimen exposed to the X-ray beam. A few membrane systems fulfil the requirements in the natural state. So far the nerve myelin sheath bas provided the most detailed X-ray diffraction patterns, and these have been interpreted (Finean and Burge 1963) to provide an outline of molecular organization that may be of general significance in membrane structure. Electron microscope studies of mammalian tissues indicate that retinal rods and cones should provide a diffracting system if the retina can be suitable exposed. In some tissues such as muscle and kidney, mitochondria tend to be elongated, aligned, and. close packed, and the tristae fairly regularly and closely spaced so as to provide a possible diffracting system, but diffraction studies of mitochondria in intact hydrated tissue have not yet been reported. The studies reported by Worthington (1960) were of mitochondria in intact but dried insect flight muscle.


Flight Muscle Diffract System Ciliary Membrane Insect Flight Muscle Residual Membrane 
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  1. Finean, J. B., 1960: Electron microscope and X-ray diffraction studies of the effects of dehydration on the structure of nerve myelin. I. Peripheral nerve and II. Optic nerve. J. Biophys. Biochem. Cytol. 8, 13 and 31.PubMedCrossRefGoogle Scholar
  2. Finean, J. B., and R. E. Bürge, 1963: The determination of the Fourier Transform of the myelin layer from a study of swelling phenomena. J. Mol. Biol. 7, 672.PubMedCrossRefGoogle Scholar
  3. Finean, J. B., and A. Martonosi, 1965: The action of phospholipase C on muscle microsomes: A correlation of electron microscope and biochemical data. Biochim. Biophys. Acta 90, 547.Google Scholar
  4. Finean, J. B., F. S. Sjöstrand, and E. Steinmann, 1953: Submicroscopic organisation of some layered lipoprotein structures. (Nerve myelin, retinal rods and chloroplasts.) Exper. Cell Res. 5, 537.Google Scholar
  5. Silvester, N. R., 1964: The cilia of Tetrahymena pyriformis: X-ray diffraction by the ciliary membrane. J. Mol. Biol. 8, 11.PubMedCrossRefGoogle Scholar
  6. Worthington, C. R., 1960: Discrete low-angle X-ray diffraction from air-dried mitochondria. J. Mol. Biol. 2, 327.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1967

Authors and Affiliations

  • J. B. Finean
    • 1
  1. 1.Department of Medical Biochemistry and PharmacologyThe UniversityBirmingham 15England

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