The Effects of Negative Stains on Lipids and Proteins Observed in the Electron Microscope

  • R. W. Horne
Conference paper


Negative staining techniques have been applied to a wide variety of biological structures, including bacteria, viruses, sub-cellular components, proteins and lipids (cf. Horne 1965). Phospholipids in the form of lecithin as well as mixtures of lecithin and cholesterol have been studied at the molecular level by Bangham and Horne (1964) and Lucy and Glauert (1964). Moreover, modifications of the phospholipid biomolecular leaflet by various chemical agents were also observed.


Phosphatidyl Ethanolamine Chemical Agent Negative Staining Ammonium Molybdate Macromolecular Structure 
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  5. Rothfield, L., and R. W. Horne: (To be published.)Google Scholar

Copyright information

© Springer-Verlag/Wien 1967

Authors and Affiliations

  • R. W. Horne
    • 1
  1. 1.Agricultural Research CouncilInstitute of Animal PhysiologyBabraham, CambridgeEngland

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