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The Diffusion of Ions from a Phospholipid Model Membrane System

  • A. D. Bangham
  • M. M. Standish
  • J. C. Watkins
  • G. Weissmann

Abstract

The recognition that biological cells exploit the surface-active properties of lipids to define anatomical membranes has, in recent years, encouraged many workers to develop and study model systems based upon the orientation of lipids at interfaces (Bangham 1963). A considerable advance was made when Mueller, Rudin, TiTien and Wescott (1964) and Haydon (quoted in Taylor 1963), simultaneously and independently reported a technique for the preparation of isolated bimolecular lipid membranes separating two aqueous compartments. Such preparations, although somewhat fickle, have enabled a variety of physical parameters to be measured. The technique lends itself pre-eminently to electrical studies of a.c. and d.c. resistances and of capacitance (Thompson 1966, Hanai, Haydon and Taylor 1964). The major criticism of the technique, however, is that the precise composition of the “black” (bimolecular) membrane is in some doubt, since it has not been found possible to spread the membranes in the absence of a relatively large mole fraction of a “filler” hydrocarbon and of water insoluble solvents. Indeed, according to Clement s and Wilson (1962), if as little as 1% of the lipid mass in a membrane contains non-polar compounds, e.g. chloroform, the membrane may be considered to be in a fully anaesthetized state.

Keywords

Liquid Crystal Aqueous Compartment Liquid Crystalline Structure Model Membrane System Study Model System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 1967

Authors and Affiliations

  • A. D. Bangham
    • 1
  • M. M. Standish
    • 1
  • J. C. Watkins
    • 1
  • G. Weissmann
    • 1
  1. 1.Agricultural Research CouncilInstitute of Animal PhysiologyBabraham, CambridgeEngland

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