Enzymes, Drugs and Transport Phenomena

  • Karl H. BeyerJr.
  • John E. Baer
Conference paper


Other contributors to this Symposium on Biophysics and Physiology of Biological Transport have dealt with the physicochemical aspects of membrane transport, what the membranes of cells and their organelles look like, how they behave physiologically, and the biochemical interpretation of the transport of ¡sodium and potassium across such structures. Background reviews of certain of these topics are readily available in the writings of Anderson and Ussing on active transport (1960), concepts of carrier transport and their pharmacological corollaries by Wilbrandt and Rosenberg (1961), the morphological and molecular aspects (Brown and Danielli 1954, Sjöstrand 1963), and the relation of structure and enzymatic activity to active transport (Lethninger 1959, Skou 1963, and Wheeler and Whittam 1962).


Tubular Secretion Carbonic Anhydrase Inhibitor Ethacrynic Acid Adenosine Triphosphatase Clearance Ratio 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, B., and H. H. Ussing, 1960: A comprehensive treatise. Comparative Biochemistry, Vol. II, M. Florkiu and H. S. Mason, editors, New York, Academic Press, pp. 371–402.Google Scholar
  2. Baer, J. E., A. Y. Brooks, R. M. Noll, and K. H. Beyer, 1962: Effect of hydrochlorothiazide on renal electrolyte gradient in glucose diuresis and experimental diabetes insipidus. J. Pharmacol. 137, 319–323.Google Scholar
  3. Baer, J. E. and K. H. Beyer, 1966: Ann. Rev. Pharmacol. 6, 261–292.PubMedCrossRefGoogle Scholar
  4. Beyer, K. H., R. H. Painter, and V. D. Wiebelhaus, 1950: Enzymatic factors in renal tubular secretion of phenol red. Amer. J. Physiol. 161, 259–267.PubMedGoogle Scholar
  5. Beyer, K. H., 1958: The mechanism of action of chlorothiazide. Ann. N. Y. Acad. Sci. 71, 363–379.PubMedCrossRefGoogle Scholar
  6. Beyer, K. H. and J. E. Baer, 1961: Physiological basis for the action of newer diuretic agents. Pharmacol. Rev. 13, 517–562.PubMedGoogle Scholar
  7. Beyer, K. H. and J. E. Baer, 1962: Effects of drugs on active transport. Ciba Foundation Symposium on Enzymes and Drug Action, edited by J. L. Mougar and A. V. S. de Reuck, pp. 60–82, J. & A. Churchill, London.Google Scholar
  8. Beyer, K. H. and J. E. Baer: In preparation.Google Scholar
  9. Bonting, S. L., K. A. Simon, and H. M. Hawkins, 1961: Studies on sodium-potassium-activated adenosine triphosphatase. I. Quantitative distribution in several tissues of the cat. Arch. Biochem. Biophys. 95, 416–423.PubMedCrossRefGoogle Scholar
  10. Brown, R., and J. J. Danielli, editors, 1954: Active transport and secretion. Symposia of Society for Experimental Biology No. 8, New York, Academic Press.Google Scholar
  11. Burg, M. B., and J. Orloff, 1962: Effect of strophanthidin on electrolyte content and PAH accumulation of rabbit kidney slices. Amer. J. Physiol. 202, 565–571.PubMedGoogle Scholar
  12. Cade, J. R., R. J. Shalhoub, M. Canessa-Fischer, and R. F. Pitts, 1961: Effect of strophanthidin on the renal tubules of dogs. Amer. J. Physiol. 200, 373–379.PubMedGoogle Scholar
  13. Clapp, J. R., J. F. Watson, and R. W. Berliner, 1963: Effect of carbonic anhydrase inhibition on proximal tubular bicarbonate reabsorption. Amer. J. Physiol. 205, 693–696.PubMedGoogle Scholar
  14. Duggan, D. E., and R. M. Noll, 1965: Effects of ethacrynic acid and cardiac glycosides upon a membrane adenosine triphosphatase of renal cortex. Arch. Biochem. Biophys. 109, 388–396.CrossRefGoogle Scholar
  15. Duggan, D. E., 1966: The accumulation of chlorothiazide and related saluretic agents by isolated renal tubules. J. Pharmacol. 152, 122–129.Google Scholar
  16. Farber, S. J., J. D. Alexander, and D. P. Earle, 1951: The effect of intravenously administered digoxin on water and electrolyte excretion and on renal functions. Circulation 4, 378–386.PubMedGoogle Scholar
  17. Giebisch, G., 1958: Electrical potential measurements on single nephrons of necturus. J. Cell. Comp. Physiol. 51, 221–239.CrossRefGoogle Scholar
  18. Giebisch, G. 1960: Measurements of electrical potentials and ion fluxes on single renal tubules. Circulation 21, 879–891.PubMedGoogle Scholar
  19. Koefoed-Johnson, V., and H. H. Ussing, 1958: The nature of the frog skin potential. Acta Physiol. Scand. 42, 298–308.CrossRefGoogle Scholar
  20. Lehninger, A. L., 1959: Relation of oxidation, phosphorylation and active transport to the structure of mitochondria. Symposium Mol. Biol. Chicago, U. Chicago Press, Chicago, p. 122–136.Google Scholar
  21. Maren, T. H., 1952: Pharmacological and renal effects of Diamox (6063), a new carbonic anhydrase inhibitor. Trans. N. Y. Acad. Sci. 15, 53.PubMedGoogle Scholar
  22. Mudge, G. W., and J. V. Taggart, 1950: Effect of 2,4-dinitrophenol on renal transport mechanisms in the dog. Amer. J. Physiol. 161, 173–180.PubMedGoogle Scholar
  23. Nechay, B. R., and L. M. J. Pardee, 1965: Inhibition of N’-methylnicotinamide secretion by ouabain in the chicken kidney. J. Pharmacol. 147, 270–276.Google Scholar
  24. Orloff, J., and M. Burg, 1960: Effect of strophanthidin on electrolyte excretion in the chicken. Amer. J. Physiol. 199, 49–54.PubMedGoogle Scholar
  25. Palmer, R. F., and B. R. Nechay, 1964: Biphasic renal effects of ouabain in the chicken: correlation with a microsomal Na+-K+ stimulated ATP-ase. J. Pharmacol. 146, 92–98.Google Scholar
  26. Rennick, B., and A. Farah, 1956: Studies on the renal tubular transport of tetraethylammonium ion in the dog. J. Pharmacol. 116, 287–295.Google Scholar
  27. Schanker, L. S., 1962: Passage of drugs across body membranes. Pharm. Rev. 14, 501–530.PubMedGoogle Scholar
  28. Sjöstrand, F. S., 1963: A comparison of plasma membrane, cytomembranes and mitochondrial membrane elements with respect to ultrastructural features. J. Ultrastruct. Res. 9, 561–580.CrossRefGoogle Scholar
  29. Skou, J. C., 1963: Enzymatic aspects of active linked transport of Na+ and K+ across the cell membrane. Proceedings of First International Pharmacological Meeting, C. A. M. Hogben, editor, 4, 41–69, Pergamon Press, New York.Google Scholar
  30. Solomon, S., 1957: Transtubular potential differences of rat kidney. J. Cell. Comp. Physiol. 49, 351–365.CrossRefGoogle Scholar
  31. Tanabe, T., I. Tsunemi, Y. Abiko, and H. Dazai, 1961: On the diuresis from the unilateral kidney produced by ouabain injected directly into the renal artery. Arch. Int. Pharmacodyn. 133, 452–462.PubMedGoogle Scholar
  32. Wheeler, K. P., and R. Whillam, 1962: Some properties of a kidney adenosine triphosphatase relevant to active cation transport. Biochem. J. 85, 495–507.PubMedGoogle Scholar
  33. White, H. I., and D. Rolf, 1963: An analysis of the effects of meralluride and ouabain on sodium excretion by stop-flow and slow-flow techniques. J. Pharmacol. 141, 326–332.Google Scholar
  34. Wilbrandt, W. and T. Rosenberg, 1961: The concept of carrier transport and its corollaries in pharmacology. Pharmacol. Rev. 13, 109–183.PubMedGoogle Scholar
  35. Wilde, W. S., and H. P. Jay, 1960: Renal tubular action of ouabain on Na and K transport during stop-flow technique. J. Pharmacol. 130, 232–238.Google Scholar
  36. Windhager, E. E., G. Whittembury, D. E. Oken, H. J. Schatzmann, and A.K. Solomon, 1959: Single proximal tubules of the necturus kidney, III. Dependence of H2O movement on NaCl concentration. Amer. J. Physiol. 197, 313–318.PubMedGoogle Scholar
  37. Windhager, E. E. and G. Giebisch, 1961: Micropuncture study of renal tubular transfer of sodium chloride in the rat. Amer. J. Physiol. 200, 581–590.PubMedGoogle Scholar
  38. Wirz, H., B. Hargitay und W. Kuhn, 1951: Lokalisation des Konzentrierungsprozesses in der Niere durch direkte Kryoskopie. Helv. physiol. et. pharmacol. acta 9, 196–207.Google Scholar

Copyright information

© Springer-Verlag/Wien 1967

Authors and Affiliations

  • Karl H. BeyerJr.
    • 1
  • John E. Baer
    • 1
  1. 1.The Merck Sharp & Dohme Research LaboratoriesWest PointUSA

Personalised recommendations