Differentiation—Dependent Changes of the Nicotinic Acetylcholine Receptor and Other Synapse-Associated Proteins
Developmentally regulated changes were followed by analyzing the appearance of synapse-associated proteins of the electric organ of Torpedo marmorata. At early stages of development acetylcholine receptor and acetylcholine esterase are distributed diffusely over the myotube surface. With differentiation they become increasingly restricted to the central cell surface. This process occurs before axons contact the electrocytes. As soon as axons begin to contact electrocytes one finds a rapid increase in acetylcholine receptor concentration, which is shortly followed by an increased synthesis of 17 S AChE. The final stages of the synapse formation coincide with increasing amounts of a hydrophobic 6 S AChE and increasing amounts of Mr 43 000 polypeptides, suggesting that the appearance of these components is linked to the maturation of receptor and synapse function.
Unable to display preview. Download preview PDF.
- Changeux, J. P.: The acetylcholine receptor: An “allosteric” membrane protein (The Harvey Lectures, Series 75), pp. 85–254. Academic Press. 1981.Google Scholar
- Fambrough, D.M.: Control of acetylcholine receptors in skeletal muscle. Physiol. Rev. 59 165–227 (1979).Google Scholar
- Froehner, S. C., Gulbrandsen, V, Hyman, C., Yeng, A. Y, Neubig, R. R., Cohen, J. B.: Immunofluorescence localization at the mammalian neuromuscular junction of the Mr43 000 protein of Torpedo postsynaptic membranes. Proc. Natl. Acad. Sci. U.S.A. 78, 5230–5234 (1981).CrossRefPubMedCentralPubMedGoogle Scholar
- Lo, M. M. S., Garland, P. B., Lamprecht,J., Barnard, E. A.: Rotational mobility of the membrane-bound acetylcholine receptor of Torpedo electric organ measured by phosphorescence depolarization. FEBS Lett. 111, 407–412 (1980).Google Scholar
- Wennogle, L. P., Changeux,J.-P.: Transmembrane orientation of proteins present in acetylcholine receptor-rich membranes from Torpedo marmorata studied by selective proteolysis. Fur. J.Biochem. 106, 381–393 (1980).Google Scholar
- Witzemann, V., Richardson, G. P., Boustead, C.: Characterization and distribution of acetylcholine receptors and acetylcholinesterase during electric organ development in Torpedo marmorataNeuroscience. 1982 (in press).Google Scholar