Summary
The mode of action of curare, a well-known competitive antagonist of acetylcholine at the nicotinic receptor, was examined with the single channel recording technique. Curare can open cholinergic channels in rat myotubes, as suggested by Ziskind and Dennis (1978). Moreover another curare molecule can then block the curare-activated open channel, in line with previous results concerning such a mode of action. In adult rat muscle, the partial agonist activity of curare can also be demonstrated, though it is much weaker than in embryonic muscle. It is also shown that in adult muscle cell, the conductance of the channel (activated either by ACh or by curare) is 50–60 pS, i.e. higher than in the myotubes (35 pS).
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References
Adams, P.R.: Voltage jump analysis of procaine action at frog end-plate. J. Physiol. 268, 291–318 (1977).
Ascher, P., Large, W., Rang, H: Studies on the mechanisms of action of acetylcholine antagonists on rat parasympathetic ganglion cells. J. Physiol. 295, 139–170 (1979).
Bekoff, A., Betz, W.J.: Physiological properties of dissociated muscle fibres obtained from innervated and denervated adult rat muscle. J. Physiol. 271 25–40 (1977).
Colquhoun, D., Dreyer, F., Sheridan, R. E.: The actions of tubocurarine at the frog neuromuscular junction. J. Physiol. 293, 247–284 (1979).
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., Sigworth, F.J.: Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflüger’s Arch. 391, 85–100 (1981).
Hamill, O. P., Sakmann, B.: Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells. Nature (Lond.) 294, 462–464 (1981).
Katz, B., Miledi, R.: A re-examination of curare action at the motor endplate. Proc. roy. Soc. B 203, 119–133 (1978).
Magleby, K. L., Stevens, C. F.: A quantitative description of end-plate currents. J. Physiol. 223, 173–197 (1972).
Marty, A., Neild, T, A scher, P.: Voltage sensitivity of acetylcholine currents in Aplysia neurones in the presence of curare. Nature (Lond.) 261, 501–503 (1976).
Sakmann, B., Brenner, H.R.: Change in synaptic channel gating during neuromuscular development. Nature (Lond.) 276, 401–402 (1978).
Sakmann, B., Patlak, J., Neher, E.: Single acetylcholine-activated channels show burst-kinetics in presence of sensitizing concentrations of agonist. Nature (Lond.) 286, 71–73 (1980).
Trautmann, A.: Curare can open and block ionic channels associated with cholinergic receptors. Nature (Lond.) 298, 272–275 (1982).
Ziskind, L., Dennis, M.J.: Depolarizing effect of curare on embryonic rat muscles. Nature (Lond.) 276, 622–623 (1978).
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© 1983 Springer-Verlag Wien
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Trautmann, A. (1983). Tubocurarine, a Partial Agonist for Cholinergic Receptors. In: Goldstein, M., Jellinger, K., Riederer, P. (eds) Basic Aspects of Receptor Biochemistry. Journal of Neural Transmission, vol 18. Springer, Vienna. https://doi.org/10.1007/978-3-7091-4408-4_34
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DOI: https://doi.org/10.1007/978-3-7091-4408-4_34
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