Summary
L-Valine (VAL) binding is saturable, reversible, linear within a protein concentration of 0.2 to 0.8 mg prot./ml, pH dependent (pH-optimum 7.1 to 7.4), temperature dependent with extremely low binding at 0–4° C; the assay runs at 37 °C; it is ion dependent (Bmax: CaCl2 (5 mM): + 100%; NaCl (5 mM): −50%; very sensitive to TRITON X-100 (Bmax at 0.01%: − 70%); N-ethylmaleimide (0.5 mM): Competitive inhibition; no change in nonspecific binding; there are no effects by puromycine plus GTP; VAL binds to synaptic membranes of human frontal cortex with KD-values several fold lower than that observed with homogenates, the distribution of VAL-binding is different in various human brain areas and can be inhibited by L-leucine, L-isoleucine, L-serine, DL-threonine, glycine and D-valine, while aromatic amino acids, β-alanine, GABA, taurine, glutamate, proline and γ-glutamyltaurine have no effects at 10−3 M.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Baudry, M., Lynch, G.: Regulation of glutamate receptors by cations. Nature 282, 748–749 (1979).
Bennett, J. P., Snyder, S. H.: Serotonin and lysergic acid diethylamide binding in rat brain membranes: relationship to postsynaptic serotonin receptors. Mol. Pharmacol. 12, 373–389 (1976).
Bergman, C., Bergman, J.: Electrogenic responses induced by neutral amino acids in endoderm cells from Xenopus embryo. J. Physiol. 318, 259–278 (1981).
Enna, S.J., Snyder, S. H.: Properties of y-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions. Brain Res. 100, 81–97 (1975).
Kienzl, E., Riederer, P., Jellinger, K., Kleinberger, G.: Antagonisierung der Ammoniakwirkung auf Serotoninrezeptoren durch L-Valin. Leber, Magen, Darm 12, 179–182 (1982 a).
Kienzl, E., Riederer, P., Jellinger, K., Kleinberger, G.: 14C-L-Valine Binding to Membranes of the Frontal Cortex in Hepatic Encephalopathy. J. Neural Transm. 55, 309–315 (1982 b).
Krueger, J. M., Cagan, R. H.: Biochemical Studies of Taste Sensation. Binding of L-[3H]-Alanine to sedimentable fraction from catfish barbel epithelium. J. Biol. Chem. 251, 88–97 (1976).
Roberts, P.J., Sharif, N. A.: Radioreceptor binding studies with glutamate and aspartate. In: Glutamate as a Neurotransmitter (Di Chiara, G., Gessa, G.L., eds.), pp. 295–305. New York: Raven Press. 1981.
Young, A. B.., Snyder, S. H.: The glycine synaptic receptor: Evidence that strychnine binding is associated with the ionic conductance mechanism. Proc. Natl. Acad. Sci. USA 71, 4002–4005 (1974).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Springer-Verlag Wien
About this paper
Cite this paper
Riederer, P., Kienzl, E., Jellinger, K., Noller, H., Kleinberger, G. (1983). General Properties of 14C-L-Valine-Binding to Human Brain Tissue. In: Goldstein, M., Jellinger, K., Riederer, P. (eds) Basic Aspects of Receptor Biochemistry. Journal of Neural Transmission, vol 18. Springer, Vienna. https://doi.org/10.1007/978-3-7091-4408-4_29
Download citation
DOI: https://doi.org/10.1007/978-3-7091-4408-4_29
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-4410-7
Online ISBN: 978-3-7091-4408-4
eBook Packages: Springer Book Archive