The Hemagglutinin Gene of Influenza Viruses
The gene order for several influenza viruses was established by identifying the RNAs and proteins of different influenza recombinant viruses using polyacrylamide gel and serologic techniques. Partial maps of many viruses containing the HO, HI, H2, H3 or Hsw hemagglutinins showed that gene (RNA) 4 always codes for hemagglutinin.
The ts defect of 7 mutant groups was identified by analyzing recombinants in which the ts gene was exchanged. One hemagglutinin mutant, ts 61S, which was shown by this technique to contain a single ts lesion (in the hemagglutinin), failed to synthesize fully glycosylated hemagglutinin molecules at the nonpermissive temperature.
Comparison of the RNA patterns of different swine viruses isolated last fall and winter from pigs in Wisconsin showed major differences in the hemagglutinin genes and in genes coding for nonsurface proteins. The RNA pattern of one swine virus isolated from man seems to identical with those of viruses isolated from several pigs.
KeywordsInfluenza Virus Pulse Label Nonpermissive Temperature Chase Period Swine Virus
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