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The Delineation of Antigenic Determinants of the Hemagglutinin of Influenza a Viruses by Means of Monoclonal Antibodies

  • Walter Gerhard
Part of the Topics in Infectious Diseases book series (TIDIS, volume 3)

Summary

The antigenicity of various viral hemagglutinins (HA) of the HO and H1 subtypes was analyzed by means of monoclonal anti-HA antibodies of BALB/c origin which were produced in vitro. Anti-HA(PR8) antibodies were able to discriminate 44 (groups of) determinants, one specific for the HA of PR8 and the others present (in a crossreactive form) on a variable number of HA of the heterologous influenza virus strains. It was estimated that the 9 antigenically related HA included in the analysis represent a minimum of (2)7 antigenic determinants that are recognized by the BALB/c immune system, implying that the individual HA may contain approximately 15 to 65 distinct determinants.

Keywords

Reactivity Type Antibody Clone Antigenic Determinant Antibody Population Antigenic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. CRUMPTON, M. J. (1974) in `The Antigens’ (Ed., Sela, M.) Vol. II, 1–78. Academic Press.Google Scholar
  2. FAZEKAS DE ST. GROTH, S. (1967) Cold Spring Harbor Symposia on Quantative Biology, Vol. 32, 525–536.CrossRefGoogle Scholar
  3. GERHARD, W. (1976) J. Exp. Med. 144, 985–995.PubMedCrossRefGoogle Scholar
  4. GERHARD, W., BRACIALE, T. J. and KLINMAN, N. R. (1975) Eur. J. Immunol. 5, 720.PubMedCrossRefGoogle Scholar
  5. KLINMAN, N. R. and ASCHINAZI, G. (1971) J. Immunol. 106, 1338–1344.PubMedGoogle Scholar
  6. LAVER, W. G., DOWNIE, J. C. and WEBSTER, R. G. (1974) Virology 59, 230–244.PubMedCrossRefGoogle Scholar
  7. ROBBINS, J. B., HAIMOVICH, J. and SELA, M. (1967) Immunochemistry 4, 11–22.Google Scholar
  8. ROSENTHAL, I. D., HAYASHI, K. and NOTKINS, A. L. (1973) Appl. Microbiol. 2S, 567573.Google Scholar
  9. VIRELIZIER, J. L., ALLISON, A. C. and SCHILD, G. C. (1974) J. exp. Med. 140, 1571 1578.Google Scholar
  10. WRIGLEY, N. G., LAVER, W. G. and DOWNIE, J. C. (1977) J. Mol. Biol. 109, 405–421.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 1978

Authors and Affiliations

  • Walter Gerhard

There are no affiliations available

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