The Purification and Structural Characterization of the Fusion Factor of Sendai Virus

  • M. J. Gething
  • J. M. White
  • M. D. Waterfield
Part of the Topics in Infectious Diseases book series (TIDIS, volume 3)


The fusion factor (F) and the hemagglutinin-neuraminidase (HN) of Sendai virus have been purified by lectin affinity chromatography on a Lens culimris (LcH)-Sepharose column. Separation was achieved by sequential elution with two different detergents containing a methyl mannoside. HN was eluted with the zwitterionic detergent Empigen BB and F with the anionic detergent sodium deoxycholate. F was further purified by molecular sieving on Ultrogel AcA34 and separated into its two component chains (F1 apparent M.W. 53,000 and F2 apparent M.W. 15,000) by molecular sieving on Sephadex G-100 after reduction of disulfide bonds. The amino terminus of F1 was PHE-PHE-GLY-ALA-VAL-ILE-GLY-ILEILE-ALA-. This sequence shows identity at six positions with the amino terminal sequence of the small polypeptide chain (HA2) of the influenza virus hemagglutinin. The amino terminal ten residues of F1 and HA2 are extremely hydrophobic and are both generated by proteolytic cleavage of an inactive precursor polypeptide. Structure and function correlations of this cleavage are discussed.


Cyanogen Bromide Sendai Virus Fusion Activity Galactose Oxidase Influenza Virus Hemagglutinin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. AMINOFF, D. (1961) Biochem. J. 81, 384–392.PubMedGoogle Scholar
  2. BLOBEL, G. and DOBBERSTEIN. B. (1975) J. Cell. Biol. 67. 835–851.PubMedCrossRefGoogle Scholar
  3. BRAUER, A. W., MARGOLIES, M. N. and HABER, E. (1975) Biochemistry 14, 3029–3035.PubMedCrossRefGoogle Scholar
  4. CRITCHLEY, D. R. (1974) Cell 3, 121–125.PubMedCrossRefGoogle Scholar
  5. CUATRECASAS, P. (1970) J. Biol. Chem. 245, 3059–3065.PubMedGoogle Scholar
  6. HOMMA, M and OHUCHI, M (1973) J. Virol. 12, 1457–1465.PubMedGoogle Scholar
  7. OZAWA, M., ASANO, A. and OKADA, Y. (1976) Febs Lett. 70, 145–149.PubMedCrossRefGoogle Scholar
  8. PISANO, J. J. and BRONZERT, T. J. (1969) J. Biol. Chem. 244, 5597–5607.PubMedGoogle Scholar
  9. SEGREST, J. P. and FELDMANN, R. J. (1974) J. Mol. Biol. 87, 853–858.PubMedCrossRefGoogle Scholar
  10. SCHEID, A. and CHOPPIN, P. W. (1974) Virology 57, 475–490.PubMedCrossRefGoogle Scholar
  11. SCHEID, A. and CHOPPIN, P. W. (1976) Virology 69, 265–277.PubMedCrossRefGoogle Scholar
  12. SMITHIES, 0., GILSON, D., FANNING, E. M., GOODFLEISH, R. M., GILMAN, J. G. and BALLANTYNE, D. C. (1971) Biochemistry 10, 4912–4921.CrossRefGoogle Scholar
  13. TOMITA, M. and MARCHESI, V. T. (1975) Proc. Nat. Acad. Sci. USA. 72, 2964–2968.CrossRefGoogle Scholar
  14. TOZAWA, H., WATANABE, M. and ISHIDA, N. (1973) Virology 55, 242–253.PubMedCrossRefGoogle Scholar
  15. WATERFIELD, M. D., SKEHEL, J. J., NAKASHIMA, Y., GURNETT, A. and BILHAM, T.(1977)this volume.Google Scholar

Copyright information

© Springer-Verlag Wien 1978

Authors and Affiliations

  • M. J. Gething
  • J. M. White
  • M. D. Waterfield

There are no affiliations available

Personalised recommendations