The sequence within the two polyadenylation sites of the A4 amyloid peptide precursor stimulates the translation

  • F. de Sauvage
  • V. Kruys
  • J. N. Octave
Part of the Key Topics in Brain Research book series (KEYTOPICS)


cDNA probes specific for the A4 amyloid peptide precursor hybridize with a 3.2–3.4 kb mRNA doublet which can be attributed to the use of two polyadenylation sites. Different chimeric mRNAs were synthesized by in vitro transcription of the coding region of the chicken lysozyme or the chloramphenicol acetyl transferase followed by two 3′ untranslated regions of the A4 amyloid peptide precursor mRNA using the two possible polyadenylation sites. In vivo translation of these mRNA constructs in Xenopus oocytes indicates that the long mRNAs using the second polyadenylation site produce higher amounts of proteins as compared to the short mRNAs. This effect on the translation is not related to a higher stability of the long mRNA in oocytes.


Down Syndrome Northern Blot Analysis Polyadenylation Site Chimeric RNAs Chicken Lysozyme 
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Copyright information

© Springer-Verlag Wien 1990

Authors and Affiliations

  • F. de Sauvage
    • 1
  • V. Kruys
    • 2
  • J. N. Octave
    • 1
  1. 1.Laboratoire de NeurochimieUniversité Catholique de LouvainBelgium
  2. 2.Université Libre de BruxellesBelgium

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