Aged dogs: an animal model to study beta-protein amyloidogenesis
The morphology, incidence and distribution of plaques and diffuse amyloid deposits in the brains of seven old dogs (18.5–26.5 years of age) were examined on tissue sections immunocytochemically stained with two monoclonal antibodies to two distinct epitopes of the beta-protein. Amyloid deposits were found in all seven brains examined. Amyloid occurred in three morphological forms: 1. focal amyloid deposits (plaques), 2. large diffuse amyloid deposits and 3. amyloid angiopathy. The number of these deposits was comparable to the numbers of all three types of amyloid deposits seen in the brains of people with Alzheimer’s disease. The number and type of morphological forms of the amyloid deposits depends on topography and the age of the animals. The number of plaques was highest in the brains of the animals 18.5–21 years of age. The oldest animals (21.5, 24 and 26.5 years of age) had a smaller number of amyloid deposits. With age, the number of plaques decreased in superficial layers of the cerebral cortex (II–III) and increased in the deeper layers (IV–VI). In the oldest animals, diffuse amyloid deposits in the deeper layers of the cortex predominated. Our studies suggest that the frequency and the extent of amyloid deposits in the brains of aged dogs are much wider than so far appreciated. It thus appears that aged dogs may be suitable as an animal model for the study of pathomechanisms involved in beta-protein amyloidogenesis.
KeywordsAmyloid Deposit Senile Plaque Paired Helical Filament Deep Cortical Layer Brain Amyloidosis
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