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Subcellular distribution of acetylcholinesterase in Alzheimer’s disease: abnormal localization and solubilization

  • Conference paper
Neurotransmitter and Dementia

Part of the book series: Journal of Neural Transmission ((NEURAL SUPPL,volume 30))

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Summary

AChE activity was detected mainly in membrane-bound fractions in the frontal cortex of autopsied control or Alzheimer brain as well as rat cerebral cortex. However, the distribution of AChE among various membrane fractions was different between control and Alzheimer brains. The highest specific activity was detected in the fraction enriched with senile plaque, which was obtained from the Alzheimer brain by sonication, solubilization with detergent and centrifugation on a sucrose density gradient. The senile plaque enriched fraction was incubated with purified collagenase or protease and centrifuged at 100,000 g for 60 min. More than 50% of AChE activity was detected in the supernatant fraction. AChE in the supernatant solution showed a property of G4 isozyme. AChE might probably be anchored to the senile plaque through its collagen tail and be solubilized with collagenase or protease, producing a G4 isozyme.

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Author information

Authors and Affiliations

  1. Department of Neurology, Kyoto University Hospital, Shogoin Sakyo-ku, Kyoto 606, Japan

    Dr. S. Nakamura

  2. Department of Neurology, Faculty of Medicine, Kyoto University, Kyoto, Japan

    Dr. S. Nakamura, S. Kawashima, S. Nakano, T. Tsuji & W. Araki

Authors
  1. Dr. S. Nakamura
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  2. S. Kawashima
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  3. S. Nakano
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  4. T. Tsuji
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  5. W. Araki
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Editor information

Editors and Affiliations

  1. Department of Psychiatry and Neurochemistry, Gothenburg University, Hisings Backa, Sweden

    C. G. Gottfries

  2. 3rd Division of Internal Medicine, Hiroshima University Hospital, Hiroshima, Japan

    S. Nakamura

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© 1990 Springer-Verlag

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Nakamura, S., Kawashima, S., Nakano, S., Tsuji, T., Araki, W. (1990). Subcellular distribution of acetylcholinesterase in Alzheimer’s disease: abnormal localization and solubilization. In: Gottfries, C.G., Nakamura, S. (eds) Neurotransmitter and Dementia. Journal of Neural Transmission, vol 30. Springer, Vienna. https://doi.org/10.1007/978-3-7091-3345-3_2

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  • DOI: https://doi.org/10.1007/978-3-7091-3345-3_2

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82190-9

  • Online ISBN: 978-3-7091-3345-3

  • eBook Packages: Springer Book Archive

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