Summary
Tryptophan is transported into the brain by a complex system, experimental and kinetic analysis of which favours a carrier mechanism functioning in association with diffusion (3-parameter model), as distinct from a system comprising two carriers (4-parameter model). The Lineweaver-Burk method is limited as a means of distinguishing between these alternative models. A statistical (linearization) technique represents an improvement over the graphical method in providing the best estimates of the parameters, as well as a measure of the variance (error mean square) of each fit to the data. However, even with this a distinction between alternative models ultimately depends upon analysis of a variety of kinetic experiments.
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Sourkes, T.L. (1979). Kinetics of Tryptophan Transport Into the Brain. In: Baumann, P. (eds) Transport Mechanisms of Tryptophan in Blood Cells, Nerve Cells, and at the Blood-Brain Barrier. Journal of Neural Transmission, vol 15. Springer, Vienna. https://doi.org/10.1007/978-3-7091-2243-3_9
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DOI: https://doi.org/10.1007/978-3-7091-2243-3_9
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