Summary
The kinetics of the influx of tryptophan and phenylalanine into guineapig intestinal rings have been examined. The transfer of these two amino acids can be described by a single transport system, each amino acid having an affinity constant, Kt, of about 4 mM for the influx mechanism. Mutual inhibition studies have shown that the inhibitory constant of each of the amino acids is also 4 mM.
Although fully competitive inhibition between the two amino acids occurs, the inhibition of the influx of the amino acids by sugars exhibits kinetics of the “pseudo-competitive” type. Such behaviour is compatible with an allosteric interaction between two different binding sites, one for each class of compounds. The lack of correlation between the inhibitory potency of a given sugar and its rate of transfer, as testified by a comparison of the effects of galactose and β-methyl-glucoside on phenylalanine influx, can be reconciled with the “allosteric-interaction hypothesis”, but specifically repudiates any theory that attempts to explain such interactions in a way that requires such a correlation. The fact that allosteric interactions are retained in cells preloaded with sodium also precludes a primary role for sodium in the mechanism of such interactions.
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References
Alvarado, F.: Transport of sugars and amino acids in the intestine: Evidence for a common carrier. Science 151, 1010–1013 (1966).
Alvarado, F.: Hypothesis for the interaction of phlorizin and phloretin with membrane carriers for sugars. Biochim. biophys. Acta 135, 483–495 (1967).
Alvarado, F.: Amino acid transport in hamster small intestine: Site of inhibition by D-galactose. Nature 219, 276–277 (1968).
Alvarado, F.: Interrelation of transport systems for sugars and amino acids in small intestine. In: Intestinal Transport of Electrolytes, Amino Acids and Sugars (Armstrong, W.McD., Nunn, A.S., eds.), pp. 281–315. Springfield: Ch. C Thomas. 1971.
Alvarado, F., Mahmood, A.: Cotransport of organic solutes and sodium ions in the small intestine: a general model. Amino acid transport. Biochemistry 13, 2882–2890 (1974).
Chez, R. A., Schultz, S. G., Curran, P. F.: Effect of sugars on transport of alanine in intestine. Science 153, 1012–1013 (1966).
Cleland, W. W.: The statistical analysis of enzyme kinetic data. Adv. Enzymol. 29, 1–32 (1967).
Curran, P. F., Schultz, S. G., Chez, R. A., Fuisz, R. E.: Kinetic relations of the Na-amino acid interaction at the mucosal border of the intestine. J. gen. Physiol. 50, 1261–1286 (1967).
Frizzell, R. A., Schultz, S. G.: Distinction between galactose and phenylalanine effects on alanine transport in rabbit ileum. Biochim. biophys. Acta 233, 485–488 (1971).
Inui, Y., Christensen, H. N.: Discrimination of single transport systems. The Na+-sensitive transport of neutral amino acids in the Ehrlich cell. J. gen. Physiol. 50, 203–224 (1966).
Kimmich, G. A., Randies, J.: Interaction between Na+-dependent transport systems for sugars and amino acids. Evidence against a role for the sodium gradient. J. memb. Biol. 12, 47–68 (1973).
Lison, L.: Statistique appliquée à la biologie expérimentale, p. 169. Paris: Gauthier-Villars. 1968.
Munck, B. G., Rasmussen, S. N.: Characteristics of rat jejunal transport of tryptophan. Biochim. biophys. Acta 389, 261–280 (1975).
Murer, H., Sigrist-Nelson, K., Hopfer, U.: On the mechanism of sugar and amino acid interaction in intestinal transport. J. biol. Chem. 250, 7392–7396 (1975).
Neame, K. D., Richards, T. G.: Elementary kinetics of membrane carrier transport, p. 79. Oxford: Blackwell. 1972.
Newey, H., Smyth, D. H.: Effects of sugars on intestinal transfer of amino acids. Nature 202, 400–401 (1964).
Robinson, J. W. L.: Role of intracellular ion concentrations on non-electrolyte influx. Gastroent. clin. biol. 2, 337–338 (1978).
Robinson, J. W. L., Alvarado, F.: Interaction between the sugar and amino acid transport systems at the small intestinal brush border: A comparative study. Pflügers Arch.-Europ. J. Physiol. 326, 48–75 (1971).
Robinson, J. W. L., Alvarado, F.: Comparative aspects of the interactions between sugar and amino acid transport systems. In: Intestinal Permeation (Kramer, M., Lauterbach, F., eds.), pp. 145–162. Amsterdam: Excerpta Medica. 1977.
Robinson, J. W. L., Felber, J.-P.: Compartments of the uptake of amino acids by intestinal fragments during in vitro incubation. Gastroenterologia (Basel) 104, 335–342 (1965).
Robinson, J. W. L., Sepulveda, F. V.: Kinetics of sodium-activated phenylalanine influx in the guinea-pig intestine in vitro. J. Physiol. (London) 266, 42P–43P (1977).
Semenza, G.: On the mechanism of mutual inhibition among sodiumdependent transport systems in the small intestine: A hypothesis. Biochim. biophys. Acta 241, 637–649 (1971).
Sepúlveda, F. V., Smith, M. W.: Discrimination between different entry mechanisms for neutral amino acids in rabbit ileal mucosa. J. Physiol. (London) 282, 73–90 (1978).
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Robinson, J.W.L., Alvarado, F. (1979). Interactions Between Tryptophan, Phenylalanine and Sugar Transport in the Small Intestinal Mucosa. In: Baumann, P. (eds) Transport Mechanisms of Tryptophan in Blood Cells, Nerve Cells, and at the Blood-Brain Barrier. Journal of Neural Transmission, vol 15. Springer, Vienna. https://doi.org/10.1007/978-3-7091-2243-3_11
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DOI: https://doi.org/10.1007/978-3-7091-2243-3_11
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