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Methods

  • Peter Böck
  • Robert Kramar
  • Margit Pavelka
Part of the Cell Biology Monographs book series (CELLBIOL, volume 7)

Abstract

Cell fractionation, a technique that arose from the early studies of Claude (1946 a, b), Hogeboom et al. (1948), and Schneider (1948), has become one of the most potent tools of biochemical research. The recognition of mitochondria as powerhouses of the cell is inconceivable without this technical approach. The sharp outlining of mitochondria and mitochondrial fractions against lysosomes and peroxisomes during the next decade (de Duve and his school, see section I.A.) became possible by refinement of these methods, especially the development of density gradient centrifugation.

Keywords

Acid Oxidase Sedimentation Coefficient Peroxidatic Activity Peroxisomal Membrane Phenazine Methosulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Wien 1980

Authors and Affiliations

  • Peter Böck
    • 1
  • Robert Kramar
    • 2
  • Margit Pavelka
    • 3
  1. 1.Anatomisches InstitutTechnische Universität MünchenFederal Republic of Germany
  2. 2.Institut für Medizinische ChemieUniversität WienAustria
  3. 3.Institut für Mikromorphologie und ElektronenmikroskopieUniversität WienAustria

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