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Protease Activated Receptor-1 and Brain Edema Formation in Glioma Models

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Brain Edema XV

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 118))

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Abstract

Objective: Our previous studies showed that thrombin contributes to brain edema in gliomas. The present study investigated the role of a thrombin receptor, protease activated receptor-1 (PAR-1), in edema formation in glioma models.

Methods: These experiments were performed in Fischer 344 rats, PAR-1 knockout mice, and wild-type C57BL/6 mice controls. F98 glioma cells were infused into the right caudate. Animals were euthanized and the brains were used for measurements of brain edema and PAR-1 expression.

Results: In rats, implantation of glioma cells resulted in significant brain edema in the ipsilateral hemisphere (82.6 ± ;1.4 vs. 78.1 ± 0.9 % in the contralateral hemisphere, p < 0.01). By Western blot analysis and RT-PCR, we found that both protein and mRNA levels of PAR-1 were upregulated in the glioma (p < 0.01). In mice, implantation of glioma cells also caused brain edema in the ipsilateral hemisphere (p < 0.05). Glioma-induced brain edema was less in PAR-1 knockout mice (day 12: 79.4 ;± 1.3 vs. 81.5 ± 1.1 % in the wild-type mice, p < ;0.05).

Conclusion: PAR-1 plays a role in glioma-induced brain edema. Clarification of the role of PAR-1 in edema formation should help to develop new therapeutic strategies for gliomas.

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Acknowledgments

This study was supported by grants NS-017760, NS-039866, and NS-057539 from the National Institutes of Health (NIH) and 0840016 N from the American Heart Association (AHA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and AHA. Drs. Xie and Gong were supported by NSFC-30901549 and NSFC-30872675 from the China National Natural Science Foundation.

Conflict of InterestWe declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan, R5018 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200, USA

    Qing Xie, Guohua Xi, Ye Gong, Richard Keep, Karin Muraszko & Ya Hua MD

  2. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China

    Qing Xie & Ye Gong

Authors
  1. Qing Xie
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  2. Guohua Xi
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  3. Ye Gong
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  4. Richard Keep
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  5. Karin Muraszko
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  6. Ya Hua MD
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Corresponding author

Correspondence to Ya Hua MD .

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Editors and Affiliations

  1. School of Medicine, Dept. Neurological Surgery, Nihon University, Oyaguchikami-machi 30-1, Tokyo, 173-8610, Japan

    Yoichi Katayama

  2. School of Medicine, Departments of Neurological Surgery and, Nihon University, 30-1 Oyaguchi Kamimachi, Itabashi-ku, Tokyo, 173-8610, Japan

    Takeshi Maeda

  3. , Department of Clinical Laboratory, Namegata District General Hospital, 98-8 Inouefujii, Namegata 98-8, Ibaraki, 311-3516, Japan

    Toshihiko Kuroiwa

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Xie, Q., Xi, G., Gong, Y., Keep, R., Muraszko, K., Hua, Y. (2013). Protease Activated Receptor-1 and Brain Edema Formation in Glioma Models. In: Katayama, Y., Maeda, T., Kuroiwa, T. (eds) Brain Edema XV. Acta Neurochirurgica Supplement, vol 118. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1434-6_35

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  • DOI: https://doi.org/10.1007/978-3-7091-1434-6_35

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-1433-9

  • Online ISBN: 978-3-7091-1434-6

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