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Brain Edema and Blood–Brain Barrier Opening After Photothrombotic Ischemia in Rat

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Brain Edema XV

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 118))

Abstract

We have examined the time course of brain edema and the blood–brain barrier opening in rat after basal ganglia ischemia induced by photothrombotic occlusion of the small vessels within the caudate–putamen. Male SD rats were anesthetized, and Rose Bengal dye was intravenously injected. The left caudo-putamen was exposed to cold white light for 5–10 min via a stereotaxically implanted optic fiber. Ischemic brain edema and the blood–brain barrier, as well as the histological changes, were assessed at various times during the following 6 weeks. Local cerebral blood flow was measured 90 min after photothrombosis by quantitative autoradiography. A round infarct with thrombosed parenchymal vessels surrounded by a layer of selective neuronal death was formed within the caudo-putamen. The ischemic lesion turned into a lacune over a period of 6 weeks. A central zone of markedly reduced blood flow and a surrounding oligemic zone were observed 90 min after light exposure. Early blood–brain barrier opening with edema was observed as early as 4 h after photothrombosis, peaked at day 1, and disappeared at 7 days after photothrombosis. In a model of lacunar infarction, we observed an early and transient brain edema and blood–brain opening after onset of ischemia.

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Conflict of Interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Clinical Medicine, Namegata District General Hospital, 98-8 Inouefujii, Namegata, Ibaraki, 311-3516, Japan

    Toshihiko Kuroiwa MD

  2. Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA

    Toshihiko Kuroiwa MD

  3. Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI, USA

    Guohua Xi MD, Ya Hua MD & Richard F. Keep

  4. Department of Anesthesiology, Henry Ford Health System, Detroit, MI, USA

    Tavarekere N. Nagaraja & Joseph D. Fenstermacher

Authors
  1. Toshihiko Kuroiwa MD
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  2. Guohua Xi MD
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  3. Ya Hua MD
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  4. Tavarekere N. Nagaraja
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  5. Joseph D. Fenstermacher
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  6. Richard F. Keep
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Corresponding author

Correspondence to Toshihiko Kuroiwa MD .

Editor information

Editors and Affiliations

  1. School of Medicine, Dept. Neurological Surgery, Nihon University, Oyaguchikami-machi 30-1, Tokyo, 173-8610, Japan

    Yoichi Katayama

  2. School of Medicine, Departments of Neurological Surgery and, Nihon University, 30-1 Oyaguchi Kamimachi, Itabashi-ku, Tokyo, 173-8610, Japan

    Takeshi Maeda

  3. , Department of Clinical Laboratory, Namegata District General Hospital, 98-8 Inouefujii, Namegata 98-8, Ibaraki, 311-3516, Japan

    Toshihiko Kuroiwa

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Kuroiwa, T., Xi, G., Hua, Y., Nagaraja, T.N., Fenstermacher, J.D., Keep, R.F. (2013). Brain Edema and Blood–Brain Barrier Opening After Photothrombotic Ischemia in Rat. In: Katayama, Y., Maeda, T., Kuroiwa, T. (eds) Brain Edema XV. Acta Neurochirurgica Supplement, vol 118. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1434-6_2

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  • DOI: https://doi.org/10.1007/978-3-7091-1434-6_2

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-1433-9

  • Online ISBN: 978-3-7091-1434-6

  • eBook Packages: MedicineMedicine (R0)

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