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Paracoccidioidomycosis: Advance Towards a Molecular Vaccine

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Molecular Vaccines

Abstract

The P10 peptide is a conserved sequence in the gp43 diagnostic antigen of most isolates of Paracoccidioides brasiliensis. P10 is promiscuously presented by three different MHC class II molecules from mouse haplotypes, and also by 90 % of Caucasian HLA-DR antigens. It elicits an IFN-γ-dependent protective anti-fungal response and is a candidate for a peptide vaccine. Immunization with P10 of mice intratracheally challenged with virulent yeasts rendered significant protection measured by colony-forming units in the lungs. A predominant Th-1 immune response was obtained. Different adjuvants enhanced the activity of the peptide and P10-primed dendritic cells were highly effective in clearing fungal infection. Gene therapy with plasmids expressing P10 and IL-12 elicited a protective Th-1 immune response without causing hyperinflammation or fibrosis. In a long-term experiment, vaccination with pP10 and pIL-12 virtually eliminated all fungal elements and preserved a normal lung parenchyma.

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Acknowledgement

The series of studies on the peptide vaccine to P. brasiliensis and gene therapy were sponsored by the Fundação de Amparo à Pesquisa do Estado de São Paulo, São Paulo, Brazil.

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Authors and Affiliations

  1. Cell Biology Division, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, SP, Brazil

    Luiz R. Travassos MD, PhD

  2. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil

    Glauce M. G. Rittner PhD & Carlos P. Taborda PhD

  3. Laboratory of Medical Mycology, Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, SP, Brazil

    Carlos P. Taborda PhD

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  1. Luiz R. Travassos MD, PhD
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  2. Glauce M. G. Rittner PhD
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  3. Carlos P. Taborda PhD
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Correspondence to Luiz R. Travassos MD, PhD .

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  1. Institute for Molecular Vaccines, Heidelberg, Germany

    Matthias Giese

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Travassos, L.R., Rittner, G.M.G., Taborda, C.P. (2013). Paracoccidioidomycosis: Advance Towards a Molecular Vaccine. In: Giese, M. (eds) Molecular Vaccines. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1419-3_14

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