Abstract
The mechanisms of enzyme–modifier interactions are treated as taxons and ranked in a way similar to that used in the biological classification of animals and plants. Two basic characters are used in the systematization of mechanisms: the allosteric linkage between substrate and modifier, if present, and the factor by which the modifier affects the catalytic constant. The states of these two characters can be combined giving rise to 17 unique combinations that are assigned to individual kinetic mechanisms embracing inhibition and nonessential activation. Essential activation is ranked as a separate group. The specific velocity plot, a method based on the same basic characters used in taxonomy, is exploited for the differential diagnosis of mechanisms during data analysis.
Mixed inhibition as defined here encompasses such a broad
range of behaviour that it may sometimes be helpful to
subdivide it further .
Nomenclature Committee of the International Union of
Biochemistry (1982). Symbolism and terminology in enzyme
kinetics. Recommendations 1981. Eur. J. Biochem. 128,
281–291 (p. 287)
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Notes
- 1.
For practical reasons, all keys are grouped in Chap. 10 because of linkages between modification mechanisms described in different chapters.
- 2.
Rewritten from a double-reciprocal equation with symbols used in this book.
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Baici, A. (2015). Taxonomy of Enzyme–Modifier Interactions and the Specific Velocity Plot. In: Kinetics of Enzyme-Modifier Interactions. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1402-5_3
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DOI: https://doi.org/10.1007/978-3-7091-1402-5_3
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