Abstract
The measurement of the ileal digestibility of amino acids is a convenient method for measuring bioavailability of amino acids in feedstuffs for pigs (Yin 2008). Because of the presence and metabolic activity of microbes, as well as the absence of amino acid absorption by the large intestine (except in the neonatal period), amino acid digestibility determined at the distal ileum is more accurate than fecal digestibility (Zebrowska 1973; Yin and McCracken 1996). That is why many scientists developed numerous surgical techniques to collect ileal digesta, including the slaughter and cannulation techniques. These latter cannulation techniques include:
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Albin DM, Wubben JE, Gabert VM (1999) Approaches to collecting ileal digesta from swine. University of Illinois Swine, Research Reports, p 21
Badawy AM, Campbell RM, Cuthbertson DP, Fell BF (1957) Changes in the intestinal mucosa of the sheep following death by humane killer. Nature 180:756–757
Badawy AM, Campbell RM, Cuthbertson DP, Fell BF, Mackie WS (1958) Further studies on changing composition of the digesta along the alimentary tract of the sheep. Total and non-protein nitrogen. Br J Nutri 12:367–383
Buraczewski S, Porter JW, Rolls BA, Zebrowska T (1971) The course of digestion of different food proteins in the rat. 2. The effect of feeding carbohydrates with proteins. Br J Nutr 25:299–306
Cori F (1925) The fate of sugar in the animal body. J Biol Chem 66:691–715
Cunninghem HM, Friend W, Nicholson JWG (1962) Note on a re-entrant fiscual for digestion studies with pigs. Can J Anim Sci 42:112–113
Cunninghem HM, Friend DW, Nicholson JWG (1963) Observation of digestion in the pig using a re-entrant intestinal fistula. Can J Anim Sci 43:215–225
Darcy B, Laplace JP, Villiers PA (1980) Collection of digesta flowing into the large intestine after post-ileocolic valve fistulation: preliminary results. Reprod Nutr Dev 20(4B):1197–1202
Darcy-Vrillon B, Laplace JP (1985) Ileal amino acid digestibility measurement in pigs fed high-fibre diet: ileo-rectal anastomosis versus ileo-colic post-valve fistulation. In: Just A, Jsrgensen H, Fernandez LA (eds) Digestive physiology in the pig. Report no. 580. National Institute of Animal Science, Copenhagen, Denmark, pp 184–187
Darcy-Vrilton B, Laplace JP (1990) Digests collection procedure may affect ileal digestibility in pigs led diets based on wheat bran or beet pulp. Anim Feed Sci Technol 27:307–316
De Lange CFM, Sauer WC, Mosenthin R, Souffrant WB (1989) The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs. J Anim Sci 67:746–754
den Hartog LA, van Leeuwen P, Huisman J, Zandstra T, van Heugten E, van Ummeren HJ, van Kleef DJ (1988) Comparison of ileal digestibility data obtained from pigs provided with a different type of cannula. In: Buraczewska L, Byraczewski S, Pastuszewska B, Zebrowka T (eds) Digestive physiology in the pig. Institute of Animal Physiology and Nutrition, Jablonna, Poland, pp 275–282
Dierick N, Vervaeke L, Decuypere J, Hernderickx HK (1983) Invloed van de aard en het niveau van ruwvezel op de schijnbare ileale en faecale droge stof, eiwit en aminozuurverteerbaarheid en op de N-retentie bij varkens. Landbouwtijdschrift 36:1661–1682
Donkoh A, Moughan PJ, Smith WC (1994) Comparison of the slaughter method and simple T-piece cannulation of the terminal ileam for determining ileal amino acid digestibility in meat and bone meal for the growing pig. Anim Feed Sci Technol 49:43–56
Easter RA, Tanksley TD (1973) A technique for re-entrant ileo-caecal cannulation of swine. J Anim Sci 36:1099–1103
Fell BF (1961) Cell shedding in the epithelium of the intestinal mucosa: fact and artifact. J Pathol Bacteriol 81:251–254
Fuller MF (1988) Methods of protein evaluation for non-ruminants. In: Orskov ER (ed) World animal science B4. Feed Science. Elsevier, Amsterdam, pp 81–101
Fuller MF, Cadenhead A (1991) Effect of the amount and composition of the diet on galactosamine flow from the small intestine. In: Verstegen MWA, Huisman J, den Hartog LA (eds) Proceedings of the 5th international symposium on digestive physiology in pigs. 24–26 April 1991. Pudoc, Wageningen, The Netherlands, pp 330–333
Fuller MF, Livingstone RM (1982) Annual report of studies Page 39 in Animal nutrition and allied sciences. Rowett Research Institute, Scotland, 45
Fuller MF, Darcy-Vrillon B, Laplace JP, Picard M, Cadenhead A, Jung J, Brown D, Franklin MF (1994) The measurement of dietary amino acid digestibility in pigs, rats and chickens: a comparison of methodologies. J Anim Feed Sci Technol 48(3–4):305–324
Graham H, Aman P (1986) Circadian variation in composition duodenal and ileal digesta from pigs fitted with T-cannula. Anim Prod 43:133–140
Green S (1988) A note on amino acids digestibility measured in pigs with pro- or post valve ileo-rectal anastomosis, fed soya-bean pea and meat meals. Anim Prod 47:317–320
Green S, Bertrand SL, Duron MJC, Maillard RA (1987) Digestibility of amino acids in maise wheat and barley meal, measured in pig with ileo-rectal anastomosis and isolation of the large intestine. J Sci Food Agric 41:29–43
Hennig U, Noel R, Herrmann U, Wiinsche J, Mehnert E (1986) Ernahrungsphysiologische Untersuchungen an Schweinen mit Ileo-Rektal- Anastomoscn. 1. Mitteilung (Nutritional physiological studies in pigs with ileo-rectal anastomoses. I. Communication). Archiv Fiir Tiererniihrung 36: 585–596
Hennig U, Wünsche J, Souffrant WB, Kreienbring F (1991) Precaecal nutrient digestibility and amino acid absorption in pigs with ileo-rectal anastomosis and i1eo-caecal re-entrant cannulas. In: Verstegen MWA, Huisman I, den Hartog LA (eds.) Digestive physiology in the pig. EAAP Publication No. 54. PUDOC Wageningen, The Netherlands, pp 304–309
Hennig U, Wünsche J, Souffrant WB, Borgmann E, Kreienbring F (1992) Comparison of methods for determining the prececal nutrient digestibility and amino acid absorption of swine with ileorectal anastomoses and ileocecal bridge cannulas. Arch Tierernahr 42(3–4):197–211
Hennig U, Wuensche J, Souffrant WB, Kreienbring F (1993) Comparison of the precaecal nutrient digestibility and amino acid absorption between pigs with ileo-rectal anastomosis and ileo-caecal re-entrant cannulae. Arch Aaim Nutr 42:197–211
Huang RL, Yin YL, Li TJ, Ti XW, Gao JP, Tao LH (2003) Techniques for implanting chronic hepatic portal vein transonic flow meter and hepatic portal vein, ileal mesenterium vein and carotid artery catheters in pigs. Acta Zoonutrimenta Sinica 15(s1):10–20
Huang RL, Yin YL, Wu GY, Zhang YG, Li TJ, Li LL, Li MX, Tang ZR, Zhang J, Wang B, He JH, Nie XZ (2005) Effect of dietary oligochitosan supplementation on ileal digestibility of nutrients and performance in broilers. Poult Sci 84:1383–1388
Jennifer E, Wubben MS, Micheller R, Smiricky MS, David M, Albin MS, Vince M, Gabert (2001) Improved procedure and cannula design for simple-T cannulation at the distal ileum in growing rigs. J Anim Sci 40(6):27–31
Jørgensen H, Jakobsen K, Eggum BO (1992) The influence of different protein, fat and mineral levels on the digestibility of fat and fatty acids measured at the terminal ileum and in feces of growing pigs. Acta Agric Scand Sect A Anim Sci 42:177–184
Just A, Sauer WC, Bech-Andersen S, Jorgensen HH, Eggum BO (1980) The influence of the hind gut microflora on the digestibility of protein and amino acids in growing pigs elucidated by addition of antibiotics to different fractions of barley. Z Tierphysiol Tierernahr U Futtermittelkd 43:83–91
Köhler T (1992) Evaluation of techniques to detect ileal digesta in pigs. PhD thesis of Animal Nutrition Department, Wageningen Agriculture University
Köhler T, Huisman J, den Hartog LA, Mosenthin R (1990) Comparison of different digesta collection methods to determine the apparent digestibility of the nutrients at the terminal ileum in pigs. J Sci Food Agric 53:465–475
Köhler T, Verstegen MWA, Huisman J, van Leeuwen P, Mosenthin R (1991a) Comparison of various techniques for measuring ileal digestibility in pigs. In: Verstegen MWA, Huisman J, den Hartog LA (eds) Digestive physiology in the pig. EAAP Publication No. 54, PUDOC, Wageningen, The Netherlands, pp 296–303
Köhler T, den Hartog A, Huisman J, Mosenthin R, Verstegen MWA (1991b) Digestibility measurements in pig by using post-valve T-caecum cannulation or end-to-side ileo-rectal anastomosis. J Anim Physiol Anim Nutr 6B:278–286
Köhler T, Denhartog LA, Huiman J, Mosenthin R, Verstegen MWA (1991c) Adaptative effect of ileo-rectal anastomosis on digestibility. In: Verstegen MWA, Huisman J, den Hartog LA (eds) Digestive physiology in the pig. EAAP Publication 563 No. 54. PUDOC, Wageningen, The Netherlands, pp 346–365
Köhler T, Mosenthin R, Verstegen MWA (1992a) Effect of ileo-rectal anastomosis and post-valve T-caecum cannulation on growing pigs. 1. Growth performance, N-balance and intestinal adaptation. Brit. J Nutr 68(1):293–303
Köhler T, Mosenthin R, Verstegen MWA (1992b) Effect of ileo-rectal anastomosis and post-valve T-caecum cannulation on growing pigs. 2. Blood variables and minerals balances. Brit J Nutr 68(1):305–315
Laplace JP, Borgida LP (1976) Physiological problems involved with re-entrant cannulas in the pig ileum: review and experimental studies. Ann Zootech 25:361–371
Laplace JP, Darcy-Vrlllon B, Picard M (1985) Evaluation de la disponibilite des acides amines: choix raisonne dune methode. J Rech Porc Fr 17:353–370
Laplace JP, Souffrant WB, Hennig U, Chabeauti E, Fevrier C (1994) Measurement of precaccal dietary protein and plant cell wall digestion in pigs; comparison of four surgical procedures for ileorectal anastomosis. Livest Prod Sci 40:313–328
Leterme P, Thewis A, Beckersa Y, Baudartb E (1990) Apparent and true ileal digestibility of amino acids and nitrogen balance measured in pigs with ileo-rectal anastomosis or T-cannulas, given a diet containing peas. J Sci Food Agric 52:485–497
Leterme P, Pirara L, Thewis A, Francois E (1991) Comparison of the rate of passage of digesta in pigs modified by ileo-rectal anastomosis or fitted with an ileal T-cannula. Digestive physiology in pigs. Proceedings of the 5th international symposium on digestive physiology in pigs, Wageningen (Doorwerth), Netherlands, vol 52, pp 361–364
Livingstone RM, Mcwilliam R (1985) The effect of terminal ileum cannulation on the performance of growing pigs. Br Vet J 141:186–191
Manolas KJ, Farmer HM, Cussen M, Welbourn RB (1983) An experimental model for simultaneous chronic sampling of portal and systemic blood and gastrointestinal lymph via cannulae in conscious swine. Cornell Vet 73:333
Moughan PJ, Smith WC (1987) A note on the effect of cannulation of the terminal ileum of the growing pig on the apparent ileal digestibility of amino acids in ground barley. Anim Prod 44:319–321
Mroz Z, Bakker GCM, Jongbloed AW, Dekker RA, Jongbloed R, van Beers A (1996) Apparent digestibility of nutrients in diets with different energy density, as estimated by direct and marker methods for pigs with or without ileo-cecal cannulas. J Anim Sci 74:403–412
Mroz Z, Jongbloed AW, Kemme PA, Everts H, Vuuren AM, van Hoste R (1991) Preliminary evaluation of a new cannulation technique (steered ileo-caecal valve) for quantitative collection of digesta from the small intestine of pigs. In: Verstegen MWA, Huisman J, den Hartog LA (eds) Digestive phyalology in the pig, EAAP Publication No. 54, PUDOC. Wageningen, The Netherlands, pp 334–339
Picard M, Bertrand S, Genin F, Maillard R (1984) Digestibility of amino acids: interest of the ileo-rectal shunt technique in pig. Journk de la Recherche Portin, en France 16:355–360
Radcliffe JS (1999) The use of steered ileo-cecal valve cannulated pigs to evaluate the effects of adding phytase or b-mannanase to the diet on amino acid, mineral and energy utilization. Doctor thesis. Virginia Polytechnic Institute and State University
Radcliffe JS, Rice JP, Pleasant RS, Apgar GA (2005) Technical note: improved technique for fitting pigs with steered ileocecal valve cannulas. J Anim Sci 83:1563–1567
Redlich J, Souffrant WB, Laplace JP, Hennig U, Berg R, Mouwen JM (1997) Morphometry of the Small Intestine in Pigs with Ileo-Rectal Anastomosis. Can J Vet Res 61:21–27
Rerat A (1972) Protein nutrition and metabolism in the growing pig. Nutr Abst Rev 42:13–39
Rerat A, Vaugelade P, Villiers P (1980) A new method for measuring the absorption of nutrients in pig: critical examination. In: Low AG, Partridge IG (eds) Current concepts of digestion and absorption in pigs. National Institute for Research in Dairying. Reading, England, Hannah Research Institute, Ayr, Scotland, Technical Bulletin 3
Rerat AA, Vaissade P, Vaugelade P (1984) Absorption kinetics of some carbohydrates in conscious pigs. 1. Qualitative aspects. Br J Nutr 51(3):505–515
Sauer WC (1976) Factors influencing amino acid digestibility for cereal grains and their components for growing monogastric animals. Ph.D. Thesis, University of Manitoba, Winnipeg, Manitoba
Sauer WC, de Lange K (1992) Novel methods for determining protein and amino acid digestibilities in feedstuffs. In: Nissen S (ed) Modern methods in protein nutrition and metabolism. Academic, San Diego, CA, pp 87–120
Sauer WC, de Lange K (1993) Novel methods for determining protein and amino acid digestibilities in feedstuffs. In: Nissen S (ed) Modern methods in protein metabolism. Academic, New York, p 87
Sauer WC, Ozimek L (1986) Digestibility of amino acids in swine: results and their practical application. A review. Livest Prod Sci 15:378–388
Sauer WC, Aherne FX, Thacker PA (1979) Comparison of amino acid digestibilities in normal and cannulated pigs. The University of Alberta 58th Annual Feeder’s Day Report, The University of Alberta Faculty of Extension, Edmonton, pp 28–29
Schroeder H, Schulz E, Oslage HJ (1989) Influence of different cannulae-techniques—simple cannula vs re-entrant cannula—on the precaecally measured digestibility of N-compounds. J Anim Physiol Anim Nutr 61(4):169–178
Souffrant WB, Schumann B, Matkowitz R, Gebhardt G (1985) Untersuchungen zur Stickstoff- und Aminos urenresorption im Diinndarm von wachsenden Schwcinen (Studies on the absorption of nitrogen and amino acids in the small intestine of growing pigs). Archiv, fir Tieremu/irung 35:781–789
Tan B, Yin YL, Liu ZQ, Li XG, Xu HJ, Kong XF, Huang RL, Tang WJ, Shinzato I, Smith SB, Wu GY (2009) Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids 37:169–175
Taverner MR, Curic DM, Rayner CJ (1983) A comparison of the extent and site of energy and protein digestion of wheat, lupin and meat and bone meal by pigs. J Sci Food Agric 34:122–128
van Leeuwen P, Huisman J, Verstegen MWA (1988a) A new technique for the collection of ileal chyme. In: Buraczewska L, Buraczewska S, Pastuszewska B, Zebrowska T (eds) Proceedings of the 4th international seminar on digestive physiology in the pig. Polish Academy of Sciences, Jablonna, Poland
Van Leeuwen P, Sauer WC, Huisman J, Van Kleef D, Den Hartog LA (1988b) Ileo-cecal re-entrant cannulation in baby pigs. J Anim Physiol Anim Nutr 59:59–63
Van Leeuwen P, van Kleef DJ, van Kempen GJM, Huisman J, Verstegen MWA (1991) The post valve T-caecum cannulation technique in pigs applicated to determine the digestibility of amino acids in maize, groundnut and sunflower meal. J Anim Physiol Anim Nutr 65:183–193
Walker WR, Morgan GL, Maxwell CV (1986) Ileal cannulation in baby pigs with a simple T-cannula. J Anim Sci 62:407–411
Wubben JE, Smiricky MS, Albin DM, Gabert VM (2001) Improved procedure and cannula design for simple-T cannulation at the distal ileum in growing pigs. Contemp Top Lab Anim Sci 40(6):27–31
Yen JT, Killefer J (1987) A method for chronically quantifying net absorption of nutrients and gut metabolites into hepatic portal vein in conscious swine. J Anim Sci 64(3):923–934
Yin Y (2008) Amino acids nutrition and metabolism in pigs. Scientia Sinica, Beijing, China
Yin YL, McCracken KJ (1996) Methodological aspects of the in vivo measurement of ileal amino acid digestibility in pigs—a review. Asian-Australas J Anim Sci 9:495–502
Yin YL, Huang RL, Zhong HY, Chen CM, Dai H (1991) Influence of different cannulation techniques on the pre-cecal digestibility of protein, amino acids and cell wall constituents from diets, containing different protein meal, in pigs. Anim Feed Sci Technol 35:271–281
Yin YL, Huang RL, Zhong HY (1993a) Comparison of ileo-ctel anastomosis and the conventional method for the measurement of ileal digestibility of protein sources and mixed diets in growing pigs. Anim Feed Sci Technol 42:297–308
Yin YL, Zhong HY, Huang RL, Chen CM, Li TJ, Pai YF (1993b) Nutritive value of feedstuffs and diets for pigs. I. Chemical composition, apparent ileal and fecal digestibility. Anim Feed Sci Technol 44:1–27
Yin YL, McEvoy J, Souffrant WB, Schulze H, McCracken KJ (2000a) Apparent digestibility (ileal and overall) of nutrients as evaluated with PVTC-cannulated or ileo-rectal anastomised pigs fed diets containing two indigestible markers. Livest Prod Sci 62:133–141
Yin YL, McEvoy J, Schulze H, McCracken KJ (2000b) Studies on cannulation method and alternative indigestible markers and the effects of food enzyme supplementation in barley-based diets on ileal and overall apparent digestibility in growing pigs. Anim Sci 70:63–72
Yin YL, Baidoo SK, Schulze H, Simmins PH (2001) Effect of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with β-glucanase and xylanase on the physiological status of gastrointestinal tract and nutrient digestibility of weaned pigs. Livest Prod Sci 71:97–107
Yin YL, Gurung Nar K, Jeaurond EA, Sharpe PH, de Lange CFM (2002) Nutrient digestibility of Canadian-developed sorghum and pearl millet grains fed to growing pigs compared to traditional cereal grains. Can J Anim Sci 82:385–391
Yin YL, Huang RL, Li TJ, Ruan Z, Xie MY, Deng ZY, Hou YQ, Wu GY (2010) Amino acid metabolism in the portal-drained viscera of young pigs: effects of dietary supplementation with chitosan and pea hull. Amino Acids 39:1581–1587
Yin FG, Zhang ZZ, Huang J, Yin YL (2010a) Digestion rate of dietary starch affects systemic circulation of amino acids in weaned pigs. Br J Nutr 103:1404–1412
Zebrowska T (1973) Digestion and absorption of nitrogenous compounds in the large intestine of pigs. Roczn Nauk Roln 95B:85–90
Zebrowska T (1978) Effect of diet and method of collection on amino acid composition of ileal digesta and digestibility of nitrogen and amino acids in pigs. Roczn Nauk Roln 99B:75–83
Zhang YC, Jrgensen H, Fernandez JA, Bach Knudsen KE (2004) Digestibility of carbohydrates in growing pigs: a comparison between the t-cannula and the steered ileo-caecal valve cannula. Arch Anim Nutr 58(3):219–231
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Wien
About this chapter
Cite this chapter
Zhang, Y. et al. (2013). Surgical Techniques Used in Amino Acid Nutrition Research. In: Blachier, F., Wu, G., Yin, Y. (eds) Nutritional and Physiological Functions of Amino Acids in Pigs. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1328-8_16
Download citation
DOI: https://doi.org/10.1007/978-3-7091-1328-8_16
Published:
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-1327-1
Online ISBN: 978-3-7091-1328-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)