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Neural Stem Cell Mapping with High-Resolution Rapid-Scanning X-Ray Fluorescence Imaging

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Cell-Based Therapies in Stroke

Abstract

Magnetic resonance imaging (MRI) of superparamagnetic iron oxide (SPIO) nanoparticle-labeled stem cells is widely used to detect cells in vivo. The sensitivity of MRI is however limited when it comes to single cell imaging, and quantifications of iron per cell is not possible. Here, we review a new use for synchrotron X-ray fluorescence (XRF) imaging, mapping element distribution following experimental stroke models in rodents, and detecting and quantifying iron in SPIO-labeled stem cells after transplantation.

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA

    Angela M. Auriat Ph.D. & Raphael Guzman M.D.

  2. Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, SK, Canada

    Helen Nichol Ph.D.

  3. Department of Neurosurgery, University of Saskatchewan, Saskatoon, SK, Canada

    Michael Kelly M.D., Ph.D.

Authors
  1. Angela M. Auriat Ph.D.
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  2. Helen Nichol Ph.D.
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  3. Michael Kelly M.D., Ph.D.
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  4. Raphael Guzman M.D.
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Corresponding author

Correspondence to Raphael Guzman M.D. .

Editor information

Editors and Affiliations

  1. , Institute of Clinical Medicine, University of Eastern Finland, Kuopio, 70210, Finland

    Jukka Jolkkonen

  2. School of Medicine, Division of MR Research, Johns Hopkins University, N Broadway 733, Baltimore, 21205, Maryland, USA

    Piotr Walczak

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© 2013 Springer-Verlag Wien

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Auriat, A.M., Nichol, H., Kelly, M., Guzman, R. (2013). Neural Stem Cell Mapping with High-Resolution Rapid-Scanning X-Ray Fluorescence Imaging. In: Jolkkonen, J., Walczak, P. (eds) Cell-Based Therapies in Stroke. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1175-8_9

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  • DOI: https://doi.org/10.1007/978-3-7091-1175-8_9

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-1174-1

  • Online ISBN: 978-3-7091-1175-8

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